N ‐glycolylneuraminic acid knockout reduces erythrocyte sequestration and thromboxane elaboration in an ex vivo pig‐to‐human xenoperfusion model
ConclusionsThe lack of Neu5Gc expression attenuated erythrocyte loss but did not prevent profound early onset thrombocytopenia or platelet activation, although TXB2 levels were decreased in the presence of Neu5GcKO. (Source: Xenotransplantation)
Source: Xenotransplantation - September 1, 2017 Category: Transplant Surgery Authors: Arielle Cimeno, Wessam Hassanein, Beth M. French, Jessica M. Powell, Lars Burdorf, Olga Goloubeva, Xiangfei Cheng, Dawn M. Parsell, Jagdeece Ramsoondar, Kasinath Kuravi, Todd Vaught, Mehmet C. Uluer, Emily Redding, Natalie O'Neill, Christopher Laird, Alen Tags: ORIGINAL ARTICLE Source Type: research
Encouraging experience using multi ‐transgenic xenografts in a pig‐to‐baboon cardiac xenotransplantation model
ConclusionsThe inclusion of additional human genes in genetically engineered pigs appears to confer superior xenograft outcomes. Introduction of these genes has not been associated with adverse outcomes. This multifactorial approach to genetic engineering furthers the prospect of long‐term cardiac xenograft survival and subsequent clinical application. (Source: Xenotransplantation)
Source: Xenotransplantation - September 1, 2017 Category: Transplant Surgery Authors: Joshua L. Chan, Avneesh K. Singh, Philip C. Corcoran, Marvin L. Thomas, Billeta GT Lewis, David L. Ayares, Todd Vaught, Keith A. Horvath, Muhammad M. Mohiuddin Tags: ORIGINAL ARTICLE Source Type: research
Creation of chimeric animals by blastocyst complementation; Editorial commentary
(Source: Xenotransplantation)
Source: Xenotransplantation - August 7, 2017 Category: Transplant Surgery Authors: L éo Bühler, Henk‐Jan Schuurman Tags: COMMENTARY Source Type: research
Issue Information
(Source: Xenotransplantation)
Source: Xenotransplantation - August 7, 2017 Category: Transplant Surgery Tags: ISSUE INFORMATION Source Type: research
Cover Image, Volume 24, Issue 4
The cover image, by Graziano Oldani et al., is based on Review Article Xenogeneic chimera—Generated by blastocyst complementation—As a potential unlimited source of recipient‐tailored organs, 2017;24:e12327. https://doi.org/10.1111/xen.12327 (Source: Xenotransplantation)
Source: Xenotransplantation - August 7, 2017 Category: Transplant Surgery Authors: Graziano Oldani, Andrea Peloso, St éphanie Lacotte, Raphael Meier, Christian Toso Tags: COVER IMAGE Source Type: research
Xenotransplantation literature update, May/June 2017
(Source: Xenotransplantation)
Source: Xenotransplantation - July 25, 2017 Category: Transplant Surgery Authors: Eric M. Walters, Karl Kerns, Christopher Burlak Tags: LITERATURE UPDATE Source Type: research
A highly reproducible cervical cuff technique for rat ‐to‐mouse heterotopic heart xenotransplantation
Abstract
Xenotransplantation is an effective way to solve the problem of donor shortage in clinical transplantation. However, clinical use of xenotransplantation is currently limited due to immunological challenges such as acute vascular rejection and cell‐mediated rejection. To finally surpass this immunological barrier, more preclinical research is needed into the molecular mechanisms of rejection and the possible effects of new immunosuppressants. Our aim was to create a refined, highly reproducible protocol to establish the most suitable rat‐to‐mouse heterotopic heart transplantation model using the cuff techniqu...
Source: Xenotransplantation - July 1, 2017 Category: Transplant Surgery Authors: Yaguang Li, Baiyi Xie, Maoshu Zhu, Xianguo Li, Feifei Du, Yanping Li, Minghui Li, Qian Yuan, Jianyu Hua, Mengjiao Xue, Zhenzhen Wang, Junjie Xia, Zhongquan Qi Tags: BRIEF COMMUNICATION Source Type: research
Cover Image, Volume 24, Issue 3
Generation of chimeric minipigs by aggregating 4‐ to 8‐cell‐stage blastomeres from somatic cell nuclear transfer with the tracing of enhanced green fluorescent protein. Xenotransplantation. 2017;24:e12300. https://doi.org/10.1111/xen.12300 (Source: Xenotransplantation)
Source: Xenotransplantation - June 20, 2017 Category: Transplant Surgery Authors: Huili Ji, Chuan Long, Chong Feng, Ningning Shi, Yingdi Jiang, Guomin Zeng, Xirui Li, Jingjing Wu, Lin Lu, Shengsheng Lu, Dengke Pan Tags: COVER IMAGE Source Type: research
Xenotransplantation literature update, March/April 2017
(Source: Xenotransplantation)
Source: Xenotransplantation - June 7, 2017 Category: Transplant Surgery Authors: Eric M. Walters, Sheida Etehad Asnaf, Christopher Burlak Tags: LITERATURE UPDATE Source Type: research
Issue Information
(Source: Xenotransplantation)
Source: Xenotransplantation - June 7, 2017 Category: Transplant Surgery Tags: ISSUE INFORMATION Source Type: research
Human regulatory macrophages are potent in suppression of the xenoimmune response via indoleamine ‐2,3‐dioxygenase‐involved mechanism(s)
ConclusionThis study demonstrates that human Mreg are capable of suppressing the xenoimmune response in vitro via IDO‐involved mechanism(s), suggesting their potential role as an effective immunomodulatory tool in xenotransplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - May 1, 2017 Category: Transplant Surgery Authors: Fei Guo, Min Hu, Dandan Huang, Yuanfei Zhao, Benjamin Heng, Gilles Guillemin, Chai K. Lim, Wayne J. Hawthorne, Shounan Yi Tags: ORIGINAL ARTICLE Source Type: research
Porcine C ‐peptide measurement to assess graft function in xenogeneic porcine islet transplantation; editorial commentary
(Source: Xenotransplantation)
Source: Xenotransplantation - May 1, 2017 Category: Transplant Surgery Authors: Henk ‐Jan Schuurman Tags: COMMENTARY Source Type: research
Financial aspects of organ procurement from deceased donors in the USA —Relevance to xenotransplantation
Abstract
When clinical xenotransplantation is introduced, the costs associated with acquisition of a genetically engineered pig organ are as yet unknown. How will these costs compare with those currently associated with the acquisition of deceased human organs? An understanding of the financial aspects of deceased organ and tissue procurement in the USA is therefore worthwhile. We have therefore attempted to review certain economic aspects of non‐profit and for‐profit organizations that provide cadaveric organs and/or tissues for purposes of transplantation into patients with end‐stage organ failure, cellular deficie...
Source: Xenotransplantation - May 1, 2017 Category: Transplant Surgery Authors: Ryan J. Saari, David K. C. Cooper Tags: REVIEW ARTICLE Source Type: research
Xenogeneic chimera —Generated by blastocyst complementation—As a potential unlimited source of recipient‐tailored organs
Abstract
Blastocyst complementation refers to the injection of cells into a blastocyst. The technology allows for the creation of chimeric animals, which have the potential to be used as an unlimited source of organ donors. Pluripotent stem cells could be generated from a patient in need of a transplantation and injected into a large animal blastocyst (potentially of a pig), leading to the creation of organ(s) allowing immunosuppression‐free transplantation. Various chimera combinations have already been generated, but one of the most recent steps leads to the creation of human‐pig chimeras, which could be studied at a...
Source: Xenotransplantation - May 1, 2017 Category: Transplant Surgery Authors: Graziano Oldani, Andrea Peloso, St éphanie Lacotte, Raphael Meier, Christian Toso Tags: REVIEW ARTICLE Source Type: research
Cross ‐validation of commercial enzyme‐linked immunosorbent assay and radioimmunoassay for porcine C‐peptide concentration measurements in non‐human primate serum
ConclusionsPorcine C‐peptide can be reliably measured in NHP serum using the Mercodia ELISA, making this assay interchangeable with the Millipore RIA. Inherent differences in antibody affinity and calibration factors may explain the lower ELISA values as compared to the RIA; however without access to a traceable reference standard, it is not possible to determine which assay is most accurate. Regression modeling resulted in a correction factor appropriate for conversion of ELISA data to RIA‐equivalent data facilitating comparison of assay results longitudinally and between groups. (Source: Xenotransplantation)
Source: Xenotransplantation - May 1, 2017 Category: Transplant Surgery Authors: Sarah C. Gresch, Lucas A. Mutch, Jody L. Janecek, Rebecca L. Hegstad ‐Davies, Melanie L. Graham Tags: ORIGINAL ARTICLE Source Type: research
