Optimization of a nonviral transfection system to evaluate Cox‐2 controlled interleukin‐4 expression for osteoarthritis gene therapy in vitro
ConclusionsWe consider that this approach has significant potential for translation into a useful anti‐inflammation therapy. Molecular tools such as the described therapeutic plasmid pave the way for a local‐controlled, self‐limiting gene therapy. Copyright © 2014 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - December 9, 2014 Category: Genetics & Stem Cells Authors: Annemarie Lang, Johannes Neuhaus, Moritz Pfeiffenberger, Erik Schröder, Igor Ponomarev, Yvonne Weber, Timo Gaber, Michael F. G. Schmidt Tags: Research Article Source Type: research
Development of a receptor‐targeted gene delivery system using CXCR4 ligand‐conjugated cross‐linking peptides
ConclusionsCXCR4‐targeted cross‐linking peptides should be considered as useful tools for nonviral gene delivery into tumor and mesenchymal stem cells. Copyright © 2014 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - December 9, 2014 Category: Genetics & Stem Cells Authors: Anna Egorova, Maria Bogacheva, Anastasia Shubina, Vladislav Baranov, Anton Kiselev Tags: Research Article Source Type: research
Designing a gold nanoparticle‐based nanocarrier for microRNA transfection into the prostate and breast cancer cells
ConclusionsIn conclusion, we show that AuNPs help the effective in vitro delivery of miR‐145 into cancer cells. Copyright © 2014 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - December 9, 2014 Category: Genetics & Stem Cells Authors: Asli Ekin, Omer Faruk Karatas, Mustafa Culha, Mustafa Ozen Tags: Research Article Source Type: research
Targeted inhibition of osteosarcoma tumor growth by bone marrow‐derived mesenchymal stem cells expressing cytosine deaminase/5‐fluorocytosine in tumor‐bearing mice
ConclusionsTaken together, these findings suggest that CD/5‐FC MSCs may be suitable for targeting human osteosarcoma. This article is protected by copyright. All rights reserved. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Authors: Quynh‐Anh NguyenThai, Neelesh Sharma, Do Huynh Luong, Simrinder Singh Sodhi, Jeong‐Hyun Kim, Nameun Kim, Sung‐Jong Oh, Dong‐Kee Jeong Tags: Research Article Source Type: research
Efficiency of transmembrane molecular transfer using electroporation in dependence of medium viscosity
This article is protected by copyright. All rights reserved. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Authors: Sandra Sungailaitė, Paulius Ruzgys, Ingrida Šatkauskienė, Karolina Čepurnienė, Saulius Šatkauskas Tags: Research Article Source Type: research
Fluorescent dye incorporation causes weakened gene association and intracellular aggregate formation in non‐viral carriers
ConclusionsThese results not only provide insight into the assembly of these structures, but also help to identify labeling strategies sufficient to preserve activity while enabling detailed studies of trafficking and disassembly. This article is protected by copyright. All rights reserved. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Authors: Abbygail A. Foster, Nikki L. Ross, Millicent O. Sullivan Tags: Research Article Source Type: research
Genetically engineered mesenchymal stromal cells producing TNFα have tumour suppressing effect on human melanoma xenograft
ConclusionsOur study demonstrates that tumor cells respond to hTNFα‐based treatment mediated by genetically engineered AT‐MSC/hTNFα both in vitro and in vivo. This article is protected by copyright. All rights reserved. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Authors: Silvia Tyciakova, Miroslava Matuskova, Roman Bohovic, Katarina Polakova, Lenka Toro, Svetlana Skolekova, Lucia Kucerova Tags: Research Article Source Type: research
Temporal endogenous gene expression profiles in response to lipid‐mediated transfection
ConclusionsRAP1A, PACSIN3, and HSPA6 may help lipoplex‐treated cells overcome a transcriptional shutdown due to treatment with lipoplexes and provide new targets for investigating molecular mechanisms of transfection or for enhancing transfection through cell priming or engineering of the nonviral gene delivery system. This article is protected by copyright. All rights reserved. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Authors: Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier Tags: Research Article Source Type: research
IDUA gene based therapy using phiC31 system to treat MPSI mice
ConclusionThe phiC31 mediated gene therapy resulted an important improvement in IDUA‐/‐ mice, including locomotion, but we also highlighted obstacles to be overcome to reach a long‐term gene therapy for MPSI. This article is protected by copyright. All rights reserved. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Authors: Roberta Sessa Stilhano, Priscila Keiko Matsumoto Martin, Suely Maymone Melo, Vivian Yochiko Samoto, Giovani Bravin Peres, Yara Maria Correa da Silva Michelacci, Flavia Helena Silva, Vanessa Gonçalves Pereira, Vania D'Almeida, Adriana Taveira Cruz, Miriam Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - November 1, 2014 Category: Genetics & Stem Cells Tags: Issue Information Source Type: research
Conference Calendar
(Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - October 27, 2014 Category: Genetics & Stem Cells Tags: Conference Calendar Source Type: research
Pre‐existing immunity to adeno‐associated virus (AAV)2 limits transgene expression following intracerebral AAV2‐based gene delivery in a 6‐hydroxydopamine model of Parkinson's disease
ConclusionsOur findings indicate that intracerebral AAV2‐based gene therapy is compromised in rats with pre‐existing immunity to AAV2. By contrast, a local neuroinflammatory response, caused by intrastriatal a 6‐OHDA injection, does not affect viral vector‐mediated transgene expression. Our results emphasize the importance of monitoring circulating AAV‐specific neutralizing antibodies in patients undergoing intracerebral gene therapy using AAV vectors. Copyright © 2014 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - October 27, 2014 Category: Genetics & Stem Cells Authors: Shorena Janelidze, Ulrika Nordström, Sebastian Kügler, Patrik Brundin Tags: Research Article Source Type: research
Long‐term therapeutic and reporter gene expression in lentiviral vector treated cystic fibrosis mice
ConclusionsThe present study showed that our airway pre‐treatment and gene delivery technique resulted in sustained functional CFTR expression and improved survival in CF mice. Copyright © 2014 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - October 27, 2014 Category: Genetics & Stem Cells Authors: Patricia Cmielewski, Martin Donnelley, David W Parsons Tags: Research Article Source Type: research
Targeted elimination of activated hepatic stellate cells by an anti‐epidermal growth factor‐receptor single chain fragment variable antibody‐tumor necrosis factor‐related apoptosis‐inducing ligand (scFv425‐sTRAIL)
ConclusionsEvidence is presented for the successful application of the scFv425‐sTRAIL fusion protein in the targeted elimination of activated hepatic stellate cells via EGFR and simultaneous activation of the caspase pathway. scFv425‐sTRAIL may thus represent a new therapeutic compound against liver fibrosis. Copyright © 2014 John Wiley & Sons, Ltd. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - October 27, 2014 Category: Genetics & Stem Cells Authors: Mohammad Arabpour, Klaas Poelstra, Wijnand Helfrich, Edwin Bremer, Hidde J. Haisma Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: The Journal of Gene Medicine)
Source: The Journal of Gene Medicine - October 27, 2014 Category: Genetics & Stem Cells Tags: Issue Information Source Type: research
