SARS ‐CoV‐2 infects an upper airway model derived from induced pluripotent stem cells
We have developed a protocol to generate airway epithelial basal-like cells from induced pluripotent stem cells, which simplifies the manufacture of cellular models of the human upper airways that are capable of supporting SARS-CoV-2 infection/replication and the secretion of cytokines in a manner similar to that of in vivo airways. AbstractAs one of the primary points of entry of xenobiotic substances and infectious agents into the body, the lungs are subject to a range of dysfunctions and diseases that together account for a significant number of patient deaths. In view of this, there is an outstanding need for in vitro ...
Source: Stem Cells - June 22, 2021 Category: Stem Cells Authors: Ivo Djidrovksi,
Maria Georgiou,
Grant L. Hughes,
Edward I. Patterson,
Aitor Casas ‐Sanchez,
Shaun H. Penington,
Giancarlo A. Biagini,
Marina Moya‐Molina,
Jelle Bor,
Martine J. Smit,
Git Chung,
Majlinda Lako,
Lyle Armstrong Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Characterization in mice of the resident mesenchymal niche maintaining AT2 stem cell proliferation in homeostasis and disease
In conclusion, rMC-Sca1PosFgf10Pos cells play important role in supporting AT2 stem cells proliferation and differentiation. This result sheds a new light on the subpopulations of rMCs contributing to the AT2 stem cell niche in homeostasis and in the context of pre-existing metabolic diseases. (Source: Stem Cells)
Source: Stem Cells - June 16, 2021 Category: Stem Cells Authors: Sara Taghizadeh,
Monika Heiner,
Ana Ivonne Vazquez ‐Armendariz,
Jochen Wilhelm,
Susanne Herold,
Chengshui Chen,
Jin San Zhang,
Saverio Bellusci Tags: Tissue ‐Specific Stem Cells Source Type: research
Human placenta mesenchymal stem cell protection in ischemic stroke is Angiotensin converting enzyme ‐2 (ACE‐2) and masR receptor dependent
In this study we demonstrated that hPMSC-derived ACE2 is required to protect against progressive loss of cerebral blood perfusion following ischemic stroke. This effect is also mediated through vascular endothelial Mas receptor but not AT2 receptor. Consequently, the results of our experimental stroke studies with human derived stem cells describes new paracrine function of hPMSC in which ACE2 and Mas receptor are critical, therefore, providing a novel therapeutic strategy for stroke in near future. (Source: Stem Cells)
Source: Stem Cells - June 15, 2021 Category: Stem Cells Authors: Mansoureh Barzegar,
Shantel Vital,
Karen Y. Stokes,
Yuping Wang,
J. Winny Yun,
Luke A. White,
Oleg Chernyshev,
Roger E. Kelley,
Jonathan S. Alexander Tags: Regenerative Medicine Source Type: research
Regulation of hippocampal postnatal and adult neurogenesis by adenosine A2A receptor: Interaction with brain ‐derived neurotrophic factor
We showed that A2A receptor (A2AR) activation enhances hippocampal neurogenesis. This A2AR-mediated neurogenesis was possible through an increase in the self-renewal capacity of type 1 and type 2 cells, and through neuronal committed cell protection, neuronal differentiation, and neuronal branching. A2AR activation was shown to promote brain-derived neurotrophic factor (BDNF) secretion and extracellular BDNF was essential for A2AR actions. Finally, A2AR endogenous activation was also essential for the BDNF-mediated actions in neuronal differentiation but not for self-renewal capacity. AbstractAdenosine A2A receptor (A2AR) ...
Source: Stem Cells - June 10, 2021 Category: Stem Cells Authors: Filipa F. Ribeiro,
Filipa Ferreira,
Rui S. Rodrigues,
Rita Soares,
Diogo M. Pedro,
Marta Duarte ‐Samartinho,
Rita I. Aroeira,
Elisabete Ferreiro,
Jorge Valero,
Susana Solá,
Helena Mira,
Ana M. Sebastião,
Sara Xapelli Tags: Tissue ‐Specific Stem Cells Source Type: research
Mitochondria in neurogenesis: Implications for mitochondrial diseases
During healthy neurogenesis, there is an orchestrated programming of energy metabolism, which transits from glycolysis toward oxidative phosphorylation (OXPHOS). In mitochondrial diseases, neural progenitor cells (NPCs) may fail to acquire an OXPHOS profile, leading to impaired generation and maturation of neurons. NPC metabolism may thus represent a potential target of interventions for mitochondrial neurological diseases. AbstractMitochondria are organelles with recognized key roles in cellular homeostasis, including bioenergetics, redox, calcium signaling, and cell death. Mitochondria are essential for neuronal function...
Source: Stem Cells - June 6, 2021 Category: Stem Cells Authors: Dario Brunetti,
Werner Dykstra,
Stephanie Le,
Annika Zink,
Alessandro Prigione Tags: Tissue ‐Specific Stem Cells Source Type: research
Zbtb46 ‐dependent altered developmental program in embryonic stem cell‐derived blood cell progenitors
In this study, we engineered murine Zbtb46-inducible embryonic stem cell lines and characterize their hematopoietic differentiation capacity. We found here that enforced expression of Zbtb46 strongly suppressed the myeloid blood cell development; moreover, this transcription factor negatively influenced the cell proliferation. On the other hand, ectopic expression of Zbtb46 was coupled with the enhanced production of CD105+ cells and the augmented erythroid blood cell development. AbstractZbtb46 is a recently identified dendritic cell (DC)-specific transcription factor with poorly defined biology. Although Zbtb46 is highly...
Source: Stem Cells - June 1, 2021 Category: Stem Cells Authors: Pal Boto,
Timea Beatrix Gerzsenyi,
Adel Lengyel,
Balint Szunyog,
Istvan Szatmari Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Zbtb46 ‐dependent altered developmental program in embryonic stem cell‐derived blood cell progenitors
In this study, we engineered murine Zbtb46-inducible embryonic stem cell lines and characterize their hematopoietic differentiation capacity. We found here that enforced expression of Zbtb46 strongly suppressed the myeloid blood cell development; moreover, this transcription factor negatively influenced the cell proliferation. On the other hand, ectopic expression of Zbtb46 was coupled with the enhanced production of CD105+ cells and the augmented erythroid blood cell development. AbstractZbtb46 is a recently identified dendritic cell (DC)-specific transcription factor with poorly defined biology. Although Zbtb46 is highly...
Source: Stem Cells - June 1, 2021 Category: Stem Cells Authors: Pal Boto,
Timea Beatrix Gerzsenyi,
Adel Lengyel,
Balint Szunyog,
Istvan Szatmari Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Characterization in mice of the stromal niche maintaining AT2 stem cell proliferation in homeostasis and disease
In conclusion, rMC-Sca1PosFgf10Pos cells play important role in supporting AT2 stem cells proliferation and differentiation. This result sheds a new light on the subpopulations of rMCs contributing to the AT2 stem cell niche in homeostasis and in the context of pre-existing metabolic diseases.© AlphaMed Press 2021Significance StatementThe mesenchyme is still a black box. It contains many subpopulations which remain to be characterized. In particular, the Cd31NegCd45NegEpcamNegSca1Pos (called resident mesenchymal cells (rMC-Sca1Pos)) has been reported to support epithelial stem cell self-renewal. We have used a robust and ...
Source: Stem Cells - May 29, 2021 Category: Stem Cells Authors: Sara Taghizadeh,
Monika Heiner,
Jochen Wilhelm,
Susanne Herold,
Chengshui Chen,
Jin San Zhang,
Saverio Bellusci Tags: Tissue ‐Specific Stem Cells Source Type: research
Regulation of hippocampal postnatal and adult neurogenesis by adenosine A2A receptor: interaction with brain ‐derived neurotrophic factor
We showed that A2AR activation enhances hippocampal neurogenesis. This A2AR-mediated neurogenesis was possible through an increase in the self-renewal capacity of Type 1 and Type 2 cells, and through neuronal committed cell protection, neuronal differentiation and neuronal branching. A2AR activation was shown to promote BDNF secretion and extracellular BDNF was essential for A2AR actions. Finally, A2AR endogenous activation was also essential for the BDNF-mediated actions in neuronal differentiation but not for self-renewal capacity. AbstractAdenosine A2A receptor (A2AR) activation modulates several brain processes, rangin...
Source: Stem Cells - May 28, 2021 Category: Stem Cells Authors: Filipa F. Ribeiro,
Filipa Ferreira,
Rui S. Rodrigues,
Rita Soares,
Diogo M. Pedro,
Marta Duarte ‐Samartinho,
Rita I. Aroeira,
Elisabete Ferreiro,
Jorge Valero,
Susana Solá,
Helena Mira,
Ana M. Sebastião,
Sara Xapelli Tags: Tissue ‐Specific Stem Cells Source Type: research
A preview of select articles
(Source: Stem Cells)
Source: Stem Cells - May 25, 2021 Category: Stem Cells Authors: Stuart P. Atkinson Tags: PREVIEWS Source Type: research
Cover Image
Image depicts the immunomodulatory interactions between mesenchymal stem cells (MSCs) and immune cells. With this powerful immunomodulatory ability, MSCs offer a promising strategy for attenuating COVID-19-related cytokine storm.See Song et al. beginning on page707. (Source: Stem Cells)
Source: Stem Cells - May 25, 2021 Category: Stem Cells Tags: COVER IMAGE Source Type: research
