Bone marrow ‐derived mesenchymal stem cells improve rat islet graft revascularization by upregulating ISL1
This study demonstrated that bone marrow ‐derived mesenchymal stem cells (BMSCs) led to an increase in insulin gene enhancer binding protein‐1 (ISL1) expression in islets and improved the vascular remodeling after islet transplantation in an animal model. Furthermore, the results demonstrate that ISL1 can directly regulate endogenous v ascular endothelial growth factor A (VEGFA) production in islets. These data indicate that ISL1 could be the molecular mediator that led to the effects of BMSCs on islet revascularization. AbstractRevascularization of the islet transplant is a crucial step that defines the success rate o...
Source: Stem Cells - April 3, 2021 Category: Stem Cells Authors: Ying Wang,
Jing ‐Wen Wang,
Yang Li,
Xiao‐Hui Tian,
Xin‐Shun Feng,
Shu‐Cong Zhang,
Pei‐Jun Liu,
Wu‐Jun Xue,
Jin Zheng,
Xiao‐Ming Ding Tags: ORIGINAL RESEARCH Source Type: research
Vascularization of human brain organoids
AbstractHuman brain organoids are three ‐dimensional tissues that are generated in vitro from pluripotent stem cells and recapitulate the early development of the human brain. Brain organoids consist mainly of neural lineage cells, such as neural stem/precursor cells, neurons, astrocytes, and oligodendrocytes. However, all human brain o rganoids lack vasculature, which plays indispensable roles not only in brain homeostasis but also in brain development. In addition to the delivery of oxygen and nutrition, accumulating evidence suggests that the vascular system of the brain regulates neural differentiation, migration, an...
Source: Stem Cells - March 31, 2021 Category: Stem Cells Authors: Takeshi K. Matsui,
Yuichiro Tsuru,
Koichi Hasegawa,
Ken ‐ichiro Kuwako Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research
Organoid Technology: Current Standing and Future Perspectives
Organoid generation and therapeutic potential AbstractOrganoids are powerful systems to facilitate the study of individuals ’ disorders and personalized treatments. Likewise, emerging this technology has improved the chance of translatability of drugs for pre‐clinical therapies and mimicking the complexity of organs, while it proposes numerous approaches for human disease modeling, tissue engineering, drug developmen t, diagnosis, and regenerative medicine. In this review, we outline the past/present organoid technology and summarize its faithful applications, then, we discuss the challenges and limitations encountered...
Source: Stem Cells - March 31, 2021 Category: Stem Cells Authors: Laleh Shariati,
Yasaman Esmaeili,
Shaghayegh Haghjooy Javanmard,
Elham Bidram,
Abbas Amini Tags: Tissue ‐Specific Stem Cells Source Type: research
Macrophages at the nexus of mesenchymal stromal cell potency ‐ the emerging role of chemokine cooperativity
The MSC and macrophage dyad theorem. MSCs polarize macrophages to an IL ‐10 anti‐inflammatory and regenerative functionality via secretome containing heteroplexed chemokines (and other active factors) or efferocytosis. Both macrophage IL‐10 licensing pathways ‐ MSC function dependent and independent ‐ converge to affect clinical outcomes in inflammatory tissue injury syndromes. AbstractPharmacological depletion of macrophagesin vivo with liposomal clodronate renders mice unresponsive to adoptive transfer of mesenchymal stromal cells (MSCs) for affecting outcomes of acute inflammatory pathology. This experimental ...
Source: Stem Cells - March 31, 2021 Category: Stem Cells Authors: Jacques Galipeau Tags: Regenerative Medicine Source Type: research
Organoid Technology: Current Standing and Future Perspectives
Organoid generation and therapeutic potential AbstractOrganoids are powerful systems to facilitate the study of individuals ’ disorders and personalized treatments. Likewise, emerging this technology has improved the chance of translatability of drugs for pre‐clinical therapies and mimicking the complexity of organs, while it proposes numerous approaches for human disease modeling, tissue engineering, drug developmen t, diagnosis, and regenerative medicine. In this review, we outline the past/present organoid technology and summarize its faithful applications, then, we discuss the challenges and limitations encountered...
Source: Stem Cells - March 31, 2021 Category: Stem Cells Authors: Laleh Shariati,
Yasaman Esmaeili,
Shaghayegh Haghjooy Javanmard,
Elham Bidram,
Abbas Amini Tags: Tissue ‐Specific Stem Cells Source Type: research
Engineered human cardiac microtissues: The state ‐of‐the‐(he)art
The recent advances in cardiac microtissue engineering focused on approaches to recapitulate the adult heart and disease phenotype. The microtissue platform depicted may not be representative of platform used in selected publication and is only a generalization. AbstractDue to the integration of recent advances in stem cell biology, materials science, and engineering, the field of cardiac tissue engineering has been rapidly progressing toward developing more accurate functional 3D cardiac microtissues from human cell sources. These engineered tissues enable screening of cardiotoxic drugs, disease modeling (eg, by using cel...
Source: Stem Cells - March 31, 2021 Category: Stem Cells Authors: Kayla Soon,
Omar Mourad,
Sara S. Nunes Vasconcelos Tags: Regenerative Medicine Source Type: research
Label ‐free quality control and identification of human keratinocyte stem cells by deep learning‐based automated cell tracking
A deep learning ‐based automated cell tracking (DeepACT) technology enables the evaluation of keratinocyte culture quality and the identification of keratinocyte stem cells using quantitative cell motion analysis. DeepACT comprises two main modules: identifying human keratinocytes at single‐cell resolution from phase‐contrast images of cultures through deep learning and tracking keratinocyte motion in the colony using a state‐space model. AbstractStem cell ‐based products have clinical and industrial applications. Thus, there is a need to develop quality control methods to standardize stem cell manufacturing. Her...
Source: Stem Cells - March 30, 2021 Category: Stem Cells Authors: Takuya Hirose,
Jun'ichi Kotoku,
Fujio Toki,
Emi K. Nishimura,
Daisuke Nanba Tags: Tissue ‐Specific Stem Cells Source Type: research
Sox2 controls neural stem cell self ‐renewal through a Fos‐centered gene regulatory network
Sox2 is necessary for long ‐term neural stem cell (NSC) self‐renewal and for Socs3, Fos, Jun, and Egr2 gene expression. We previously showed that Socs3 overexpression rescues Sox2‐deleted NSC self‐renewal. Fos, Jun, and Egr2 transduction into Sox2‐deleted NSC rescues self‐renewal, Fos alone being both sufficient a nd necessary. Sox2 transduction upregulates Fos and Socs3 in Sox2‐deleted cells, and Fos upregulates Socs3, defining a Sox2‐dependent regulatory network. AbstractThe Sox2 transcription factor is necessary for the long ‐term self‐renewal of neural stem cells (NSCs). Its mechanism of action is s...
Source: Stem Cells - March 29, 2021 Category: Stem Cells Authors: Miriam Pagin,
Mattias Pernebrink,
Simone Giubbolini,
Cristiana Barone,
Gaia Sambruni,
Yanfen Zhu,
Matteo Chiara,
Sergio Ottolenghi,
Giulio Pavesi,
Chia ‐Lin Wei,
Claudio Cantù,
Silvia K. Nicolis Tags: Tissue ‐Specific Stem Cells Source Type: research
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Source: Stem Cells - March 26, 2021 Category: Stem Cells Authors: Stuart P. Atkinson Tags: PREVIEWS Source Type: research
Cover Image
3D confocal imaging of a neonatal rat cochlea immunostained to detect spiral ganglion neurons (cyan) and hair cells (red). (Image credit: Dr. Michael Perny).See Roccio beginning on page375. (Source: Stem Cells)
Source: Stem Cells - March 26, 2021 Category: Stem Cells Tags: COVER IMAGE Source Type: research
Issue Information
(Source: Stem Cells)
Source: Stem Cells - March 26, 2021 Category: Stem Cells Tags: ISSUE INFORMATION Source Type: research
A preview of selected articles
(Source: Stem Cells)
Source: Stem Cells - March 26, 2021 Category: Stem Cells Authors: Stuart P. Atkinson Tags: PREVIEWS Source Type: research
