Bone marrow ‐derived mesenchymal stem cells improve rat islet graft revascularization by upregulating ISL1

This study demonstrated that bone marrow ‐derived mesenchymal stem cells (BMSCs) led to an increase in insulin gene enhancer binding protein‐1 (ISL1) expression in islets and improved the vascular remodeling after islet transplantation in an animal model. Furthermore, the results demonstrate that ISL1 can directly regulate endogenous v ascular endothelial growth factor A (VEGFA) production in islets. These data indicate that ISL1 could be the molecular mediator that led to the effects of BMSCs on islet revascularization. AbstractRevascularization of the islet transplant is a crucial step that defines the success rate of patient recovery. Bone marrow ‐derived mesenchymal stem cells (BMSCs) have been reported to promote revascularization; however, the underlying cellular mechanism remains unclear. Moreover, our liquid chromatography‐tandem mass spectrometry results showed that BMSCs could promote the expression of insulin gene enhancer bindin g protein‐1 (ISL1) in islets. ISL1 is involved in islets proliferation and plays a potential regulatory role in the revascularization of islets. This study identifies the ISL1 protein as a potential modulator in BMSCs‐mediated revascularization of islet grafts. We demonstrated that the survival rate and insulin secretion of islets were increased in the presence of BMSCs, indicating that BMSCs promote islet revascularization in a coculture system and rat diabetes model. Interestingly, we also observed that the presence of BMSCs ...
Source: Stem Cells - Category: Stem Cells Authors: Tags: ORIGINAL RESEARCH Source Type: research