LncRNA, PLXDC2 ‐OT promoted the osteogenesis potentials of MSCs by inhibiting the deacetylation function of RBM6/SIRT7 complex and OSX specific isoform
In this study, we found thatSIRT7 knockdown increased ALP activity and in vitro mineralization and promoted the expression of the osteogenic differentiation markersDSPP,DMP1,BSP,OCN, and the key transcription factorOSX in MSCs. In addition, SIRT7 could associate with RNA binding motif protein 6 (RBM6) to form a protein complex. Moreover, RBM6 inhibited ALP activity, the expression ofDSPP,DMP1,BSP,OCN, andOSX in MSCs, and the osteogenesis of MSCs in vivo. Then, the SIRT7/RBM6 protein complex was shown to downregulate the level of H3K18Ac in theOSX promoter by recruiting SIRT7 to theOSX promoter and inhibiting the expression...
Source: Stem Cells - March 10, 2021 Category: Stem Cells Authors: Huina Liu,
Lei Hu,
Guoxia Yu,
Haoqing Yang,
Yangyang Cao,
Songlin Wang,
Zhipeng Fan Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research
Electrophysiological characterization of photoreceptor ‐like cells in human inducible pluripotent stem cell‐derived retinal organoids during in vitro maturation
This study provides a comprehensive dataset describing the electrophysiological maturation of photoreceptor‐like cells in human inducible pluripotent stem cell‐derived ROs for precise and sensitive quality control during RO differentiation. AbstractRetinal organoids (ROs) derived from human inducible pluripotent stem cells (hiPSCs) exhibit considerable therapeutic potential. However, current quality control of ROs during in vitro differentiation is largely limited to the detection of molecular markers, often by immunostaining, polymerase chain reaction (PCR) assays and sequencing, often without proper functional assess...
Source: Stem Cells - March 8, 2021 Category: Stem Cells Authors: Lingyun Li,
Huan Zhao,
Haohuan Xie,
Tasneem Akhtar,
Yichuan Yao,
Yuan Cai,
Kai Dong,
Yonghao Gu,
Jin Bao,
Jutao Chen,
Mei Zhang,
Kai Zhong,
Weiping Xu,
Tian Xue Tags: Tissue ‐Specific Stem Cells Source Type: research
Mesenchymal stem cell immunomodulation: In pursuit of controlling COVID ‐19 related cytokine storm
Potential mechanism of MSC action in COVID ‐19 infected patients. SARS‐CoV‐2 enters cells through receptor‐mediated endocytosis via interactions with cell surface protein angiotensin‐converting enzyme II (ACE2) receptor with the assistance of transmembrane protease serine 2 (TMPRSS2) protease, thus triggering a complex immune respo nse involved in T cells, dendritic cells, natural killer cells and macrophages. Engineering MSCs with immunomodulatory molecules enhance the efficacy of homing to damaged tissues or cells and attenuate the cytokine storm, ultimately improving patients' outcome. AbstractThe Coronavirus ...
Source: Stem Cells - March 8, 2021 Category: Stem Cells Authors: Na Song,
Hiroaki Wakimoto,
Filippo Rossignoli,
Deepak Bhere,
Rachele Ciccocioppo,
Kok ‐Siong Chen,
Jasneet Kaur Khalsa,
Ilenia Mastrolia,
Anna Valeria Samarelli,
Massimo Dominici,
Khalid Shah Tags: Translational and Clinical Research Source Type: research
Transplanted pluripotent stem cell ‐derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration
This study assessed the ability of pluripotent stem cell derived cone photoreceptors to engraft in an end ‐stage mouse model of photoreceptor degeneration model and restore vision. Our data demonstrate that pluripotent stem cell derived cone precursors can engraft into the host retina in close apposition to the host bipolar cells. Half of the transplanted mice exhibited visual behaviour and of these, 33% showed binocular light sensitivity. The majority of retinal ganglion cells (RGCs) exhibited contrast‐sensitive light responses and motion sensitivity, however quite a few exhibited unusual responses reflecting remodell...
Source: Stem Cells - March 6, 2021 Category: Stem Cells Authors: Darin Zerti,
Gerrit Hilgen,
Birthe Dorgau,
Joseph Collin,
Marius Ader,
Lyle Armstrong,
Evelyne Sernagor,
Majlinda Lako Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
O ‐GlcNAc transferase is required to maintain satellite cell function
O ‐GlcNAcylation is a widespread nutrient sensitive posttranslational modification that satellite cells (SCs) use to maintain homeostasis and proper SC function. Ablation of SC specific O‐GlcNAc transferase (OGT) impairs adult regenerative myogenesis,in vivo cycling properties, and proliferation, presumably through a host ‐cell factor 1 (HCF1)‐mediated arrest in the cell cycle. AbstractO ‐GlcNAcylation is a posttranslational modification considered to be a nutrient sensor that reports nutrient scarcity or surplus. Although O‐GlcNAcylation exists in a wide range of cells and/or tissues, its functional role in mu...
Source: Stem Cells - March 6, 2021 Category: Stem Cells Authors: Morgan D. Zumbaugh,
Ashley E. Geiger,
Jing Luo,
Zhengxing Shen,
Hao Shi,
David E. Gerrard Tags: Tissue ‐Specific Stem Cells Source Type: research
Direct neuronal differentiation of neural stem cells for spinal cord injury repair
Diagram showing the directional neuronal differentiation of neural stem cells (NSCs). A, Spinal cord injury (SCI) microenvironment is unfavorable for the neuronal differentiation of NSCs. B, The application of functional biomaterials, myelin ‐associated inhibitor antagonists, small molecules, regenerative factors, and seed cells was used to reconstruct the SCI microenvironment and facilitate the neuronal differentiation of NSCs. C, The intrinsic neuronal differentiation abilities of NSCs were enhanced to promote neurogenesis after SCI . AbstractSpinal cord injury (SCI) typically results in long ‐lasting functional defi...
Source: Stem Cells - March 6, 2021 Category: Stem Cells Authors: Weiwei Xue,
Caixia Fan,
Bing Chen,
Yannan Zhao,
Zhifeng Xiao,
Jianwu Dai Tags: Tissue ‐Specific Stem Cells Source Type: research
Phage display targeting identifies EYA1 as a regulator of glioblastoma stem cell maintenance and proliferation
AbstractGlioblastoma (GBM) ranks among the most lethal of human malignancies with GBM stem cells (GSCs) that contribute to tumor growth and therapeutic resistance. Identification and isolation of GSCs continues to be a challenge, as definitive methods to purify these cells for study or targeting are lacking. Here, we leveraged orthogonal in vitro and in vivo phage display biopanning strategies to isolate a single peptide with GSC ‐specific binding properties. In silico analysis of this peptide led to the isolation of EYA1 (Eyes Absent 1), a tyrosine phosphatase and transcriptional coactivator. Validating the phage discov...
Source: Stem Cells - March 5, 2021 Category: Stem Cells Authors: JongMyung Kim,
Chunhua She,
Marine Potez,
Ping Huang,
Qiulian Wu,
Briana C. Prager,
Zhixin Qiu,
Shideng Bao,
Jeremy N. Rich,
James K. C. Liu Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
