LncRNA, PLXDC2 ‐OT promoted the osteogenesis potentials of MSCs by inhibiting the deacetylation function of RBM6/SIRT7 complex and OSX specific isoform

In this study, we found thatSIRT7 knockdown increased ALP activity and in vitro mineralization and promoted the expression of the osteogenic differentiation markersDSPP,DMP1,BSP,OCN, and the key transcription factorOSX in MSCs. In addition, SIRT7 could associate with RNA binding motif protein 6 (RBM6) to form a protein complex. Moreover, RBM6 inhibited ALP activity, the expression ofDSPP,DMP1,BSP,OCN, andOSX in MSCs, and the osteogenesis of MSCs in vivo. Then, the SIRT7/RBM6 protein complex was shown to downregulate the level of H3K18Ac in theOSX promoter by recruiting SIRT7 to theOSX promoter and inhibiting the expression ofOSX isoforms 1 and 2. Furthermore, lncRNAPLXDC2 ‐OT could associate with the SIRT7/RBM6 protein complex to diminish its binding and deacetylation function in theOSX promoter and its inhibitory function onOSX isoforms 1 and 2 and to promote the osteogenic potential of MSCs.
Source: Stem Cells - Category: Stem Cells Authors: Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research