Transplanted pluripotent stem cell ‐derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration

This study assessed the ability of pluripotent stem cell derived cone photoreceptors to engraft in an end ‐stage mouse model of photoreceptor degeneration model and restore vision. Our data demonstrate that pluripotent stem cell derived cone precursors can engraft into the host retina in close apposition to the host bipolar cells. Half of the transplanted mice exhibited visual behaviour and of these, 33% showed binocular light sensitivity. The majority of retinal ganglion cells (RGCs) exhibited contrast‐sensitive light responses and motion sensitivity, however quite a few exhibited unusual responses reflecting remodelling of the neural retina. Together these data suggest that pluripotent stem cell‐derived cone precursors can elicit conventional and unusual light responsiveness at advanced degeneration stages. AbstractRetinal dystrophies often lead to blindness. Developing therapeutic interventions to restore vision is therefore of paramount importance. Here we demonstrate the ability of pluripotent stem cell ‐derived cone precursors to engraft and restore light responses in thePde6brd1 mouse, an end ‐stage photoreceptor degeneration model. Our data show that up to 1.5% of precursors integrate into the host retina, differentiate into cones, and engraft in close apposition to the host bipolar cells. Half of the transplanted mice exhibited visual behavior and of these 33% showed binocular light sensitivity. The majority of retinal ganglion cells exhibited contrast‐se...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research