Characterization in mice of the stromal niche maintaining AT2 stem cell proliferation in homeostasis and disease

In conclusion, rMC-Sca1PosFgf10Pos cells play important role in supporting AT2 stem cells proliferation and differentiation. This result sheds a new light on the subpopulations of rMCs contributing to the AT2 stem cell niche in homeostasis and in the context of pre-existing metabolic diseases.© AlphaMed Press 2021Significance StatementThe mesenchyme is still a black box. It contains many subpopulations which remain to be characterized. In particular, the Cd31NegCd45NegEpcamNegSca1Pos (called resident mesenchymal cells (rMC-Sca1Pos)) has been reported to support epithelial stem cell self-renewal. We have used a robust and reproducible flow cytometry strategy associated with the alveolosphere assay to characterize further this subpopulation. Our results indicate that rMC-Sca1PosFgf10Pos-LIFs support AT2 stem cell self-renewal and differentiation. We have also compared this subpopulation to the previously described rMC-Sca1PosAxin2Pos cells. Our results indicate that they are two distinct populations. In addition, we demonst
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research