Hypoxic Conditioned Medium From Human Adipose-Derived Stem Cells Promotes Mouse Liver Regeneration Through JAK/STAT3 Signaling
In this study, it was found that treatment with the medium from hypoxic-preconditioned adipose-derived stem cells (ASCs) increased the viability of hepatotoxic hepatocytes and enhance liver regeneration in partially hepatectomized mice. In addition, the researchers first revealed that the hepatoprotective effects of hypoxic-conditioned media are mediated by persistent and uninhibited expression of signal transducer and activator of transcription 3 in the liver, which result from a decreased expression of suppressor of cytokine signaling 3. Therefore, the hypoxic preconditioning of ASCs is expected to play a crucial role in...
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Lee, S. C., Jeong, H. J., Lee, S. K., Kim, S.-J. Tags: Tissue Engineering and Regenerative Medicine, Adipose-Derived Stem Cells Source Type: research
Effects of Rho Kinase Inhibitors on Grafts of Dopaminergic Cell Precursors in a Rat Model of Parkinsons Disease
In models of Parkinson’s disease (PD), Rho kinase (ROCK) inhibitors have antiapoptotic and axon-stabilizing effects on damaged neurons, decrease the neuroinflammatory response, and protect against dopaminergic neuron death and axonal retraction. ROCK inhibitors have also shown protective effects against apoptosis induced by handling and dissociation of several types of stem cells. However, the effect of ROCK inhibitors on dopaminergic cell grafts has not been investigated. In the present study, treatment of dopaminergic cell suspension with ROCK inhibitors yielded significant decreases in the number of surviving dopa...
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Rodriguez-Pallares, J., Rodriguez-Perez, A. I., Munoz, A., Parga, J. A., Toledo-Aral, J. J., Labandeira-Garcia, J. L. Tags: Tissue Engineering and Regenerative Medicine, Neural/Progenitor Stem Cells Source Type: research
Effects of Matrix Metalloproteinases on the Performance of Platelet Fibrin Gel Spiked With Cardiac Stem Cells in Heart Repair
In this study, the effects of matrix metalloproteinase inhibition on the performance of platelet gel spiked with cardiac stem cells (cell-gel) for heart regeneration are explored. The results demonstrate that matrix metalloproteinases are required for cell-gel to exert its benefits in cardiac repair. Inhibition of matrix metalloproteinases reduces cell engraftment, host angiogenesis, and recruitment of endogenous cardiovascular cells in rats with heart attack. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Shen, D., Tang, J., Hensley, M. T., Li, T., Caranasos, T. G., Zhang, T., Zhang, J., Cheng, K. Tags: Tissue Engineering and Regenerative Medicine, Cardiac Stem Cells Source Type: research
Combined Therapy With Adipose-Derived Mesenchymal Stem Cells and Ciprofloxacin Against Acute Urogenital Organ Damage in Rat Sepsis Syndrome Induced by Intrapelvic Injection of Cecal Bacteria
In conclusion, ADMSC-assisted ciprofloxacin therapy offered an additional benefit by reducing acute urogenital organ damage in rat.
Significance
Autologous adipose-derived mesenchymal stem cell-assisted ciprofloxacin therapy offered an additional benefit by reducing acute urogenital organ damage in rats. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Sung, P.-H., Chiang, H.-J., Chen, C.-H., Chen, Y.-L., Huang, T.-H., Zhen, Y.-Y., Chang, M.-W., Liu, C.-F., Chung, S.-Y., Chen, Y.-L., Chai, H.-T., Sun, C.-K., Yip, H.-K. Tags: Adipose-Derived Stem Cells, Standards, Policies, Protocols, and Regulations for Cell-Based Therapies Source Type: research
Increased Survival and Function of Mesenchymal Stem Cell Spheroids Entrapped in Instructive Alginate Hydrogels
This study demonstrates the promise of cell-instructive biomaterials to direct survival and function of MSC spheroids for bone tissue engineering applications.
Significance
Mesenchymal stem cell (MSC) spheroids exhibit improved therapeutic potential in vitro compared with dissociated MSCs, yet spheroids are directly injected into tissues, ceding control of cell function to the extracellular matrix and potentially limiting the duration of improvement. Cell delivery using adhesive biomaterials promotes cell retention and function. These studies explored the role of adhesion to the surrounding matrix on spheroid function. Wh...
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Ho, S. S., Murphy, K. C., Binder, B. Y. K., Vissers, C. B., Leach, J. K. Tags: Mesenchymal Stem Cells, Enabling Technologies for Cell-Based Clinical Translation Source Type: research
Reducing Hepatocyte Injury and Necrosis in Response to Paracetamol Using Noncoding RNAs
The liver performs multiple functions within the human body. It is composed of numerous cell types, which play important roles in organ physiology. Our study centers on the major metabolic cell type of the liver, the hepatocyte, and its susceptibility to damage during drug overdose. In these studies, hepatocytes were generated from a renewable and genetically defined resource. In vitro-derived hepatocytes were extensively profiled and exposed to varying levels of paracetamol and plasma isolated from liver-failure patients, with a view to identifying noncoding microRNAs that could reduce drug- or serum-induced hepatotoxicit...
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Szkolnicka, D., Lucendo-Villarin, B., Moore, J. K., Simpson, K. J., Forbes, S. J., Hay, D. C. Tags: Embryo Development, Cell-Based Drug Development, Screening, and Toxicology Source Type: research
Mesenchymal Stromal Cell-Derived Extracellular Vesicles Protect the Fetal Brain After Hypoxia-Ischemia
Preterm neonates are susceptible to perinatal hypoxic-ischemic brain injury, for which no treatment is available. In a preclinical animal model of hypoxic-ischemic brain injury in ovine fetuses, we have demonstrated the neuroprotective potential of systemically administered mesenchymal stromal cells (MSCs). The mechanism of MSC treatment is unclear but suggested to be paracrine, through secretion of extracellular vesicles (EVs). Therefore, we investigated in this study the protective effects of mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) in a preclinical model of preterm hypoxic-ischemic brain injury....
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Ophelders, D. R. M. G., Wolfs, T. G. A. M., Jellema, R. K., Zwanenburg, A., Andriessen, P., Delhaas, T., Ludwig, A.-K., Radtke, S., Peters, V., Janssen, L., Giebel, B., Kramer, B. W. Tags: Mesenchymal Stem Cells, Cell-Based Drug Development, Screening, and Toxicology Source Type: research
Influence of Bone and Muscle Injuries on the Osteogenic Potential of Muscle Progenitors: Contribution of Tissue Environment to Heterotopic Ossification
This study clarified the contribution of a tissue environment created by bone or muscle injury to the formation of HO. The study also found that muscle-derived mesenchymal stem cells, but not myogenic progenitors, are involved in the formation of HO. The findings of this study could be used to strategize the prevention and treatment of HO. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Molligan, J., Mitchell, R., Schon, L., Achilefu, S., Zahoor, T., Cho, Y., Loube, J., Zhang, Z. Tags: Mesenchymal Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
Human Cartilage-Derived Progenitor Cells From Committed Chondrocytes for Efficient Cartilage Repair and Regeneration
This study reports the derivation of a population of cartilage stem/progenitor cells from fully differentiated chondrocytes under specific culture conditions, which have the potential to reassume their chondrocytic phenotype for efficient cartilage regeneration. These findings support the possibility of using in vitro amplified chondrocyte-derived progenitor cells for joint cartilage repair. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Jiang, Y., Cai, Y., Zhang, W., Yin, Z., Hu, C., Tong, T., Lu, P., Zhang, S., Neculai, D., Tuan, R. S., Ouyang, H. W. Tags: Adult Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
Expansion and Hepatic Differentiation of Adult Blood-Derived CD34+ Progenitor Cells and Promotion of Liver Regeneration After Acute Injury
This study identified and characterized a group of blood-derived progenitor cells (BDPCs) from the peripheral blood of an adult mouse. The CD34+ progenitor-dominant BDPCs were rapidly expanded and hepatically differentiated into functional hepatocyte-like cells with our established coculture system. BDPC treatment increased animal survival and produced full regeneration in a severe liver injury mouse model caused by CCl4. BDPCs could have potential for liver cell therapies. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Hu, M., Li, S., Menon, S., Liu, B., Hu, M. S., Longaker, M. T., Lorenz, H. P. Tags: Hematopoietic Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
Using miRNA-mRNA Interaction Analysis to Link Biologically Relevant miRNAs to Stem Cell Identity Testing for Next-Generation Culturing Development
In conclusion, the identified biologically relevant miRNA markers can be used to monitor stem cell stability when implementing variations in culturing procedures.
Significance
Human adult marrow stromal stem cells have shown great potential in addressing unmet health care needs. Quality assurance is imperative to make advancements in large-scale manufacturing procedures. MicroRNAs are master regulators of biological processes and potentially ideal quality markers. MicroRNA and mRNA profiling data of two human adult stem cell types were correlated to biological functions in silico. Doing this provided evidence that differe...
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Crabbe, M. A. E., Gijbels, K., Visser, A., Craeye, D., Walbers, S., Pinxteren, J., Deans, R. J., Annaert, W., Vaes, B. L. T. Tags: Mesenchymal Stem Cells, Tissue-Specific Progenitor and Stem Cells Source Type: research
Strategies for Achieving Measurement Assurance for Cell Therapy Products
This article presents the many strategies that can be used to ensure measurement confidence and discusses them in terms of how they can be applied to characterization of cell therapy products. Application of these strategies to cell measurements will help to establish qualified assays for cell characterization, which may help streamline regulatory approval and enable more efficient development of cell therapy products.
Significance
The regenerative medicine industry has identified the lack of reliable methods for measuring critical cell attributes as possibly the single greatest challenge facing the field. There are many ...
Source: Stem Cells Translational Medicine - May 23, 2016 Category: Stem Cells Authors: Simon, C. G., Lin-Gibson, S., Elliott, J. T., Sarkar, S., Plant, A. L. Tags: Standards, Policies, Protocols, and Regulations for Cell-Based Therapies Meeting Reports Source Type: research
In Reply
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - April 17, 2016 Category: Stem Cells Authors: Srour, N., Thebaud, B. Tags: Letters to the Editor Source Type: research
What Should Be Chronic: The Animal, the Model, or Both?
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - April 17, 2016 Category: Stem Cells Authors: Rubio, G. A., Elliot, S. J., Glassberg, M. K. Tags: Letters to the Editor Source Type: research
Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration
This study also demonstrated that combined transgene-free reprogramming and enzymatic dissociation allows teratoma-free transplantation of iPSC progeny in the mouse model in testing the tumorigenicity of iPSC products. Further safety assessment and improvement in iPSC specification into a mature β cell phenotype would lead to safe islet replacement therapy for diabetes. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - April 17, 2016 Category: Stem Cells Authors: El Khatib, M. M., Ohmine, S., Jacobus, E. J., Tonne, J. M., Morsy, S. G., Holditch, S. J., Schreiber, C. A., Uetsuka, K., Fusaki, N., Wigle, D. A., Terzic, A., Kudva, Y. C., Ikeda, Y. Tags: Tissue Engineering and Regenerative Medicine, Pluripotent Stem Cells Source Type: research
