Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes
In conclusion, our study has shown that osteogenic differentiation of hiPSCs from hGFs was improved by HCG-311. The mechanism might be that the nHA addition stimulates osteogenic marker expression of hiPSCs from hGFs. Our work has provided an innovative autologous cell-based bone tissue engineering approach with soft tissues such as clinically abundant gingiva.
Significance
The present study focused on patient-personalized bone tissue engineering. Human induced pluripotent stem cells (hiPSCs) were established from clinically easily derived human gingival fibroblasts (hGFs) and defined nanohydroxyapatite/chitosan/gelatin (...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Ji, J., Tong, X., Huang, X., Zhang, J., Qin, H., Hu, Q. Tags: Tissue Engineering and Regenerative Medicine, Pluripotent Stem Cells Source Type: research
Intraportal Infusion of Bone Marrow Mononuclear or CD133+ Cells in Patients With Decompensated Cirrhosis: A Double-Blind Randomized Controlled Trial
The present study assessed the effects of intraportal infusions of autologous bone marrow-derived mononuclear cells (MNCs) and/or CD133+ cells on liver function in patients with decompensated cirrhosis. We randomly assigned 27 eligible patients to a placebo, MNCs, and/or CD133+ cells. Cell infusions were performed at baseline and month 3. We considered the absolute changes in the Model for End-Stage Liver Disease (MELD) scores at months 3 and 6 after infusion as the primary outcome. The participants and those who assessed the outcomes were unaware of the treatment intervention assignments. After 6 months, 9 patients were e...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Mohamadnejad, M., Vosough, M., Moossavi, S., Nikfam, S., Mardpour, S., Akhlaghpoor, S., Ashrafi, M., Azimian, V., Jarughi, N., Hosseini, S.-E., Moeininia, F., Bagheri, M., Sharafkhah, M., Aghdami, N., Malekzadeh, R., Baharvand, H. Tags: Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells Source Type: research
Therapeutic Efficacy of Fresh, Autologous Mesenchymal Stem Cells for Severe Refractory Gingivostomatitis in Cats
This study is the first to demonstrate the safety and efficacy of fresh, autologous, adipose-derived stem cell systemic therapy for a naturally occurring, chronic inflammatory disease in cats. The findings demonstrate that this therapy resulted in complete clinical and histological resolution or reduction in clinical disease severity and immune modulation in most cats. This study also identified a potentially useful biomarker that could dictate patient enrollment and shed light on immune modulation mechanism. As a naturally occurring animal model, FCGS also provides a strategic platform for potentially translatable therapy...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Arzi, B., Mills-Ko, E., Verstraete, F. J. M., Kol, A., Walker, N. J., Badgley, M. R., Fazel, N., Murphy, W. J., Vapniarsky, N., Borjesson, D. L. Tags: Enabling Technologies for Cell-Based Clinical Translation, Mesenchymal Stem Cells Source Type: research
Magnetic Resonance Imaging of Iron Oxide-Labeled Human Embryonic Stem Cell-Derived Cardiac Progenitors
Given the limited regenerative capacity of the heart, cellular therapy with stem cell-derived cardiac cells could be a potential treatment for patients with heart disease. However, reliable imaging techniques to longitudinally assess engraftment of the transplanted cells are scant. To address this issue, we used ferumoxytol as a labeling agent of human embryonic stem cell-derived cardiac progenitor cells (hESC-CPCs) to facilitate tracking by magnetic resonance imaging (MRI) in a large animal model. Differentiating hESCs were exposed to ferumoxytol at different time points and varying concentrations. We determined that trea...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Skelton, R. J. P., Khoja, S., Almeida, S., Rapacchi, S., Han, F., Engel, J., Zhao, P., Hu, P., Stanley, E. G., Elefanty, A. G., Kwon, M., Elliott, D. A., Ardehali, R. Tags: Enabling Technologies for Cell-Based Clinical Translation, Embryo Development Source Type: research
Injection of Human Cord Blood Cells With Hyaluronan Improves Postinfarction Cardiac Repair in Pigs
This study using healthy human cord blood mononuclear cells (CB-MNCs) to treat myocardial infarction provides preclinical evidence that combined injection of hyaluronan and human CB-MNCs after myocardial infarction significantly increases cell retention in the peri-infarct area, improves cardiac performance, and prevents cardiac remodeling. Moreover, using healthy cells to replace dysfunctional autologous cells may constitute a better strategy to achieve heart repair and regeneration. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Chang, M.-Y., Huang, T.-T., Chen, C.-H., Cheng, B., Hwang, S.-M., Hsieh, P. C. H. Tags: Enabling Technologies for Cell-Based Clinical Translation, Cord Blood Stem Cells Source Type: research
Propranolol Targets Hemangioma Stem Cells via cAMP and Mitogen-Activated Protein Kinase Regulation
Infantile hemangiomas (IHs) are the most common vascular tumor and arise from a hemangioma stem cell (HemSC). Propranolol has proved efficacious for problematic IHs. Propranolol is a nonselective β-adrenergic receptor (βAR) antagonist that can lower cAMP levels and activate the mitogen-activated protein kinase (MAPK) pathway downstream of βARs. We found that HemSCs express β1AR and β2AR in proliferating IHs and determined the role of these βARs and the downstream pathways in mediating propranolol’s effects. In isolated HemSCs, propranolol suppressed cAMP levels and activated extracellula...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Munabi, N. C. O., England, R. W., Edwards, A. K., Kitajewski, A. A., Tan, Q. K., Weinstein, A., Kung, J. E., Wilcox, M., Kitajewski, J. K., Shawber, C. J., Wu, J. K. Tags: Tissue-Specific Progenitor and Stem Cells, Endodermal Stem/Progenitor Cells Source Type: research
Propranolol and Mesenchymal Stromal Cells Combine to Treat Traumatic Brain Injury
In this study, an animal model of moderate-severe TBI was treated with an acute dose of propranolol followed by a delayed dose of human mesenchymal stem cells (MSCs), resulting in improved short- and long-term measurements. These results have direct translational application. They reinforce the inevitable clinical trial of MSCs to treat TBI by demonstrating, among other benefits, a notable decrease in chronic neuroinflammation. More importantly, these results demonstrate that MSCs and propranolol, which is increasingly being used clinically for TBI, are compatible treatments that improve overall outcome. (Source: Stem Cell...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Kota, D. J., Prabhakara, K. S., van Brummen, A. J., Bedi, S., Xue, H., DiCarlo, B., Cox, C. S., Olson, S. D. Tags: Tissue-Specific Progenitor and Stem Cells, Mesenchymal Stem Cells Source Type: research
Human Periodontal Stem Cells Release Specialized Proresolving Mediators and Carry Immunomodulatory and Prohealing Properties Regulated by Lipoxins
Unresolved inflammation and tissue destruction are underlying mechanisms of periodontitis, which is linked to dysregulated polymorphonuclear neutrophil (PMN) functions. Lipoxin A4 (LXA4) is a specialized proresolving lipid mediator (SPM) that dampens excessive inflammation, promotes resolution, and protects from leukocyte-mediated tissue damage. Human periodontal ligament stem cells (hPDLSCs) represent key players during tissue regeneration and may contribute to resolution of inflammation; thus, they may represent a promising tool in regenerative dentistry. In the present study, we investigated the actions of hPDLSCs on PM...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Cianci, E., Recchiuti, A., Trubiani, O., Diomede, F., Marchisio, M., Miscia, S., Colas, R. A., Dalli, J., Serhan, C. N., Romano, M. Tags: Tissue-Specific Progenitor and Stem Cells, Perivascular Stem Cells Source Type: research
Naive Induced Pluripotent Stem Cells Generated From {beta}-Thalassemia Fibroblasts Allow Efficient Gene Correction With CRISPR/Cas9
Conventional primed human embryonic stem cells and induced pluripotent stem cells (iPSCs) exhibit molecular and biological characteristics distinct from pluripotent stem cells in the naïve state. Although naïve pluripotent stem cells show much higher levels of self-renewal ability and multidifferentiation capacity, it is unknown whether naïve iPSCs can be generated directly from patient somatic cells and will be superior to primed iPSCs. In the present study, we used an established 5i/L/FA system to directly reprogram fibroblasts of a patient with β-thalassemia into transgene-free naïve iPSCs with ...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Yang, Y., Zhang, X., Yi, L., Hou, Z., Chen, J., Kou, X., Zhao, Y., Wang, H., Sun, X.-F., Jiang, C., Wang, Y., Gao, S. Tags: Pluripotent Stem Cells, Endodermal Stem/Progenitor Cells Source Type: research
Challenging Regeneration to Transform Medicine
This article describes a Regenerative Medicine Grand Challenge that the Alliance for Regenerative Medicine recently shared with the White House’s Office of Science and Technology Policy in response to a White House call to action in scientific disciplines suggesting that the development of "universal donor tissues" should be designated as a Regenerative Medicine Grand Challenge. Such a designation would raise national awareness of the potential of regenerative medicine to address the unmet needs of many diseases and would stimulate the scientific partnerships and investments in technology needed to expedite this goal...
Source: Stem Cells Translational Medicine - January 7, 2016 Category: Stem Cells Authors: Tsukamoto, A., Abbot, S. E., Kadyk, L. C., DeWitt, N. D., Schaffer, D. V., Wertheim, J. A., Whittlesey, K. J., Werner, M. J. Tags: Perspectives Source Type: research
Volume 4 Author Index
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Tags: Volume 4 Author Index Source Type: research
Volume 4 Acknowledgments
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Tags: Volume 4 Acknowledgments Source Type: research
Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel
This study shows the therapeutic value of human adipose-derived stem cell spheroids prepared in hyarulonic acid gel. The spheroids have various benefits as an injectable cellular product and show therapeutic potential to the stem cell-depleted conditions such as diabetic chronic skin ulcer. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Mineda, K., Feng, J., Ishimine, H., Takada, H., Doi, K., Kuno, S., Kinoshita, K., Kanayama, K., Kato, H., Mashiko, T., Hashimoto, I., Nakanishi, H., Kurisaki, A., Yoshimura, K. Tags: Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells Source Type: research
Mesenchymal Stromal Cells in Animal Bleomycin Pulmonary Fibrosis Models: A Systematic Review
Idiopathic pulmonary fibrosis is an inexorably progressive lung disease with few available treatments. New therapeutic options are needed. Stem cells have generated much enthusiasm for the treatment of several conditions, including lung diseases. Human trials of mesenchymal stromal cell (MSC) therapy for pulmonary fibrosis are under way. To shed light on the potential usefulness of MSCs for human disease, we aimed to systematically review the preclinical literature to determine if MSCs are beneficial in animal bleomycin pulmonary fibrosis models. The MEDLINE and Embase databases were searched for original studies of stem c...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Srour, N., Thebaud, B. Tags: Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells Source Type: research
Concise Review: The U.S. Food and Drug Administration and Regenerative Medicine
This article provides an overview of the efforts of the U.S. Food and Drug Administration (FDA) to facilitate product development in the field commonly known was regenerative medicine. It provides an introduction to the processes by which FDA works with individual sponsors, interacts with the scientific and industry communities, participates in standards development, and develops formal FDA policy and guidance. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Witten, C. M., McFarland, R. D., Simek, S. L. Tags: Concise Review, Tissue Engineering and Regenerative Medicine Source Type: research
