A Universal and Robust Integrated Platform for the Scalable Production of Human Cardiomyocytes From Pluripotent Stem Cells
In this study, a simplified, scalable, robust, and integrated differentiation platform was developed to generate clinical grade hPSC-CMs as cell aggregates under chemically defined culture conditions. This approach resulted in approximately 100% beating CM spheroids with virtually pure (~90%) functional cardiomyocytes in 10 days from multiple hPSC lines. This universal and robust bioprocessing platform can provide sufficient numbers of hPSC-CMs for companies developing regenerative medicine technologies to rescue, replace, and help repair damaged heart tissues and for pharmaceutical companies developing advanced biologics ...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Fonoudi, H., Ansari, H., Abbasalizadeh, S., Larijani, M. R., Kiani, S., Hashemizadeh, S., Zarchi, A. S., Bosman, A., Blue, G. M., Pahlavan, S., Perry, M., Orr, Y., Mayorchak, Y., Vandenberg, J., Talkhabi, M., Winlaw, D. S., Harvey, R. P., Aghdami, N., Bah Tags: Protocols and Manufacturing for Cell-Based Therapies, Pluripotent Stem Cells Source Type: research
Fluorine-19 Labeling of Stromal Vascular Fraction Cells for Clinical Imaging Applications
Stromal vascular fraction (SVF) cells are used clinically for various therapeutic targets. The location and persistence of engrafted SVF cells are important parameters for determining treatment failure versus success. We used the GID SVF-1 platform and a clinical protocol to harvest and label SVF cells with the fluorinated (19F) agent CS-1000 as part of a first-in-human phase I trial (clinicaltrials.gov identifier NCT02035085) to track SVF cells with magnetic resonance imaging during treatment of radiation-induced fibrosis in breast cancer patients. Flow cytometry revealed that SVF cells consisted of 25.0% ± 15.8% C...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Rose, L. C., Kadayakkara, D. K., Wang, G., Bar-Shir, A., Helfer, B. M., OHanlon, C. F., Kraitchman, D. L., Rodriguez, R. L., Bulte, J. W. M. Tags: Enabling Technologies for Cell-Based Clinical Translation, Adipose-Derived Stem Cells Source Type: research
Concise Review: Understanding the Renal Progenitor Cell Niche In Vivo to Recapitulate Nephrogenesis In Vitro
Chronic kidney disease (CKD), defined as progressive kidney damage and a reduction of the glomerular filtration rate, can progress to end-stage renal failure (CKD5), in which kidney function is completely lost. CKD5 requires dialysis or kidney transplantation, which is limited by the shortage of donor organs. The incidence of CKD5 is increasing annually in the Western world, stimulating an urgent need for new therapies to repair injured kidneys. Many efforts are directed toward regenerative medicine, in particular using stem cells to replace nephrons lost during progression to CKD5. In the present review, we provide an ove...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Mari, C., Winyard, P. Tags: Concise Review, Tissue-Specific Progenitor and Stem Cells, Mesenchymal Stem Cells Source Type: research
Subcutaneous Transplantation of Neural Precursor Cells in Experimental Autoimmune Encephalomyelitis Reduces Chemotactic Signals in the Central Nervous System
This article provides evidence of interference in immune signaling within the central nervous system as a potential mechanism underlying the immunomodulatory properties of transplanted neural precursor cells. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Ravanidis, S., Poulatsidou, K. N., Lagoudaki, R., Touloumi, O., Polyzoidou, E., Lourbopoulos, A., Nousiopoulou, E., Theotokis, P., Kesidou, E., Tsalikakis, D., Karacostas, D., Grigoriou, M., Chlichlia, K., Grigoriadis, N. Tags: Tissue-Specific Progenitor and Stem Cells, Neural/Progenitor Stem Cells Source Type: research
Solution-Phase Crosstalk and Regulatory Interactions Between Multipotent Adult Progenitor Cells and Peripheral Blood Mononuclear Cells
This study documents experiments quantifying solution-phase crosstalk between multipotent adult progenitor cells (MAPCs) and peripheral blood mononuclear cells. The secretome and transcriptional changes quantified suggest mechanisms by which MAPCs are hypothesized to provide both local and systemic immunoregulation of inflammation. The potential impact of these studies includes development of a robust experimental framework to be used for preclinical evaluation of the specific mechanisms by which beneficial effects are obtained after treatment of patients with MAPCs. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Burrows, G. G., vant Hof, W., Reddy, A. P., Wilmarth, P. A., David, L. L., Raber, A., Bogaerts, A., Timmerman, L., Pinxteren, J., Roobrouck, V. D., Deans, R. J., Maziarz, R. T. Tags: Tissue-Specific Progenitor and Stem Cells, Mesenchymal Stem Cells Source Type: research
Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy
This study found that mesenchymal stem cells may crosstalk with cardiomyocytes, which causes a synergistic vascular endothelial growth factor (VEGF) release from both kinds of cells and then inhibits pathological cardiac remodeling following hypertrophic stimulation in cardiomyocytes in vitro and in vivo. Blockage of VEGF release from bone marrow-derived mesenchymal stem cells (BMSCs) abolishes the antihypertrophic actions of BMSCs in vitro and in vivo. On the contrary, VEGF administration attenuates hypertrophic signaling of calcineurin/ nuclear factor of activated T cell cytoplasmic 3 signal pathways. This study provides...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Cai, B., Tan, X., Zhang, Y., Li, X., Wang, X., Zhu, J., Wang, Y., Yang, F., Wang, B., Liu, Y., Xu, C., Pan, Z., Wang, N., Yang, B., Lu, Y. Tags: Tissue-Specific Progenitor and Stem Cells, Mesenchymal Stem Cells Source Type: research
Generation of Functional Human Cardiac Progenitor Cells by High-Efficiency Protein Transduction
The reprogramming of fibroblasts to induced pluripotent stem cells raises the possibility that somatic cells could be directly reprogrammed to cardiac progenitor cells (CPCs). The present study aimed to assess highly efficient protein-based approaches to reduce or eliminate the genetic manipulations to generate CPCs for cardiac regeneration therapy. A combination of QQ-reagent-modified Gata4, Hand2, Mef2c, and Tbx5 and three cytokines rapidly and efficiently reprogrammed human dermal fibroblasts (HDFs) into CPCs. This reprogramming process enriched trimethylated histone H3 lysine 4, monoacetylated histone H3 lysine 9, and ...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Li, X.-H., Li, Q., Jiang, L., Deng, C., Liu, Z., Fu, Y., Zhang, M., Tan, H., Feng, Y., Shan, Z., Wang, J., Yu, X.-Y. Tags: Tissue-Specific Progenitor and Stem Cells, Pluripotent Stem Cells Source Type: research
Chronic High-Fat Feeding Affects the Mesenchymal Cell Population Expanded From Adipose Tissue but Not Cardiac Atria
This study examined the effect of a high-fat diet on mesenchymal cells from cardiac and adipose tissues. It was demonstrated that a high-fat diet did not affect cardiac progenitor cells but increased the number of fibroblasts and hematopoietic cells within the adipose-derived mesenchymal cell population. (Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Perbellini, F., Gomes, R. S. M., Vieira, S., Buchanan, D., Malandraki-Miller, S., Bruyneel, A. A. N., Sousa Fialho, M. d. L., Ball, V., Clarke, K., Faggian, G., Carr, C. A. Tags: Tissue-Specific Progenitor and Stem Cells, Mesenchymal Stem Cells Source Type: research
Bone Morphogenetic Protein-2 Induces Donor-Dependent Osteogenic and Adipogenic Differentiation in Human Adipose Stem Cells
This study examined how bone morphogenetic protein-2 (BMP-2) modulates the SMAD signaling mechanism and the proliferation and differentiation outcome of human adipose stem cells (hASCs) derived from several donors. The results indicate that BMP-2 triggers molecular SMAD signaling mechanisms in hASCs and regulates differentiation processes in human serum-culture conditions. Importantly, BMP-2 has dual activity, inducing osteogenic and adipogenic differentiation, subject to hASC donor line studied. These findings explain contradictory previous results and highlight the importance of further studies to understand how signalin...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Vanhatupa, S., Ojansivu, M., Autio, R., Juntunen, M., Miettinen, S. Tags: Tissue-Specific Progenitor and Stem Cells, Adipose-Derived Stem Cells Source Type: research
Vascular Smooth Muscle Cells From Hypertensive Patient-Derived Induced Pluripotent Stem Cells to Advance Hypertension Pharmacogenomics
Studies in hypertension (HTN) pharmacogenomics seek to identify genetic sources of variable antihypertensive drug response. Genetic association studies have detected single-nucleotide polymorphisms (SNPs) that link to drug responses; however, to understand mechanisms underlying how genetic traits alter drug responses, a biological interface is needed. Patient-derived induced pluripotent stem cells (iPSCs) provide a potential source for studying otherwise inaccessible tissues that may be important to antihypertensive drug response. The present study established multiple iPSC lines from an HTN pharmacogenomics cohort. We dem...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Biel, N. M., Santostefano, K. E., DiVita, B. B., El Rouby, N., Carrasquilla, S. D., Simmons, C., Nakanishi, M., Cooper-DeHoff, R. M., Johnson, J. A., Terada, N. Tags: Pluripotent Stem Cells, Pluripotent Stem Cells Source Type: research
Regenerative Medicine Build-Out
Regenerative technologies strive to boost innate repair processes and restitute normative impact. Deployment of regenerative principles into practice is poised to usher in a new era in health care, driving radical innovation in patient management to address the needs of an aging population challenged by escalating chronic diseases. There is urgency to design, execute, and validate viable paradigms for translating and implementing the science of regenerative medicine into tangible health benefits that provide value to stakeholders. A regenerative medicine model of care would entail scalable production and standardized appli...
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Terzic, A., Pfenning, M. A., Gores, G. J., Harper, C. M. Tags: Perspectives Source Type: research
New Government Accountability Office Report on Regenerative Medicine Provides an Excellent Assessment of the Field
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - December 10, 2015 Category: Stem Cells Authors: Murphy, S. V., Atala, A. Tags: Editorial Source Type: research
The Autism Spectrum Disorders Stem Cell Resource at Children's Hospital of Orange County: Implications for Disease Modeling and Drug Discovery
(Source: Stem Cells Translational Medicine)
Source: Stem Cells Translational Medicine - October 27, 2015 Category: Stem Cells Authors: Brick, D. J., Nethercott, H. E., Montesano, S., Banuelos, M. G., Stover, A. E., Schutte, S. S., O'Dowd, D. K., Hagerman, R. J., Ono, M., Hessl, D. R., Tassone, F., Schwartz, P. H. Tags: Erratum Source Type: research
Concise Review: Stem Cell Microenvironment on a Chip: Current Technologies for Tissue Engineering and Stem Cell Biology
Stem cells have huge potential in many therapeutic areas. With conventional cell culture methods, however, it is difficult to achieve in vivo-like microenvironments in which a number of well-controlled stimuli are provided for growing highly sensitive stem cells. In contrast, microtechnology-based platforms offer advantages of high precision, controllability, scalability, and reproducibility, enabling imitation of the complex physiological context of in vivo. This capability may fill the gap between the present knowledge about stem cells and that required for clinical stem cell-based therapies. We reviewed the various type...
Source: Stem Cells Translational Medicine - October 27, 2015 Category: Stem Cells Authors: Park, D., Lim, J., Park, J. Y., Lee, S.-H. Tags: Concise Review, Tissue Engineering and Regenerative Medicine, Mesenchymal Stem Cells Source Type: research
Regeneration of Full-Thickness Rotator Cuff Tendon Tear After Ultrasound-Guided Injection With Umbilical Cord Blood-Derived Mesenchymal Stem Cells in a Rabbit Model
In this study, we investigated the therapeutic effects of ultrasound-guided human umbilical cord blood (UCB)-derived mesenchymal stem cell (MSC) injection to regenerate a full-thickness subscapularis tendon tear in a rabbit model by evaluating the gross morphology and histology of the injected tendon and motion analysis of the rabbit’s activity. At 4 weeks after ultrasound-guided UCB-derived MSC injection, 7 of the 10 full-thickness subscapularis tendon tears were only partial-thickness tears, and 3 remained full-thickness tendon tears. The tendon tear size and walking capacity at 4 weeks after UCB-derived MSC inject...
Source: Stem Cells Translational Medicine - October 27, 2015 Category: Stem Cells Authors: Park, G.-Y., Kwon, D. R., Lee, S. C. Tags: Tissue Engineering and Regenerative Medicine, Cord Blood Stem Cells Source Type: research
