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Impact of Apo E gene polymorphism on HCV therapy related outcome in a cohort of HCV Egyptian patients
Conclusion: This study supports the hypothesis of the protective impact of Apo E4 allele that favors viral clearance of HCV infection and its recovery after combined therapy, while the Apo E3 allele is considered as a particular risk factor for the chronicity in HCV patients and resistance to therapy. Whereas the Apo E2 allele confers a resistance to HCV infection at a time of exposure. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Down-regulation of circulating microRNA let-7a in Egyptian smokers
In conclusion, plasma let-7a was significantly down-regulated in the smokers, and it might be considered a candidate biomarker to discriminate between smokers and non-smokers. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Effect of vitamin A deficiency on thymosin-β4 and CD4 concentrations
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Salwa Refat El-Zayat, Hiba Sibaii, Nermine N. Mahfouz, Sara F. Sallam, Reham F. Fahmy, Azza Abd El-ShaheedAbstractVitamins are evaluated for their role in immunity. Recently, vitamin A received a particular attention as a critical micronutrient for regulating immune system. Therefore, the present study aimed to search for new about vitamin A. Forty-eight Egyptian adults aged from 18 to 42 years old from both sexes were subjected to clinical examination and nutrition questionnaire and were screened for vitamin...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Differentially expressed genes: OCT-4, SOX2, STAT3, CDH1 and CDH2, in cultured mesenchymal stem cells challenged with serum of women with endometriosis
This study shows that endometriosis serum induced molecular changes in human endometrial MSCs (EnMSCs) that might be related to altered cell behavior which may be a step in differentiation that may be completed invivo by other factors to complete the process of transition. Further researches are needed for optimization to reach differentiation. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

The sensitivity and efficacy method of PIK3CA exon 9 E545A as a high diagnostic accuracy in breast cancer
ConclusionThe results presented here demonstrate that the PIK3CA exon 9 E545A detection method has a good sensitivity and efficacy assay, which proves that the method has a high diagnostic accuracy in breast cancer. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Molecular detection and PCR-RFLP analysis using Pst1 and Alu1 of multidrug resistant Klebsiella pneumoniae causing urinary tract infection in women in the eastern part of Bangladesh
In this study, a rapid combined polymerase chain reaction and restriction fragment length polymorphism analysis was developed to identify K. pneumoniae in women, infected with urinary tract infection in the Sylhet city of Bangladesh. Analysis of 11 isolates from women at the age range of 20–55 from three different hospitals were done firstly by amplification with K. pneumoniae specific ITS primers. All of the 11 collected isolates were amplified in PCR and showed the expected 136 bp products. Then, restriction fragment length polymorphism analysis of 11 isolates were conducted after PCR amplification by 16s rRNA univer...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Identification of Mx gene nucleotide dimorphism (G/A) as genetic marker for antiviral activity in Egyptian chickens
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Mohamed S. Hassanane, Amal A.M. Hassan, Fatma M. Ahmed, Esteftah M. El-Komy, Khaled M. Roushdy, Nagwa A. HassanAbstractEgyptian chickens, representing 2 breeds and 7 strains, were genotyped using the PCR-RFLP and sequencing techniques for detection of a non-synonymous dimorphism (G/A) in exon 14 of chicken Myxovirus resistance (Mx) gene. This dimorphic position is responsible for altering Mx protein’s antiviral activity. Polymerase Chain reactions were performed using Egyptian chickens DNA and specific primer...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Genetic variability of myostatin and prolactin genes in popular goat breeds in Egypt
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Sekena H. Abdel-Aziem, K.F. Mahrous, M.A.M. Abd El-Hafez, M. Abdel MordyAbstractThe genetic polymorphisms of two functional genes named: myostatin (MSTN) and prolactin (PRL) were investigated in three goat breeds (Barki, Damascus and Zaraibi) using Sanger nucleotide sequence and restriction fragment length polymorphism (RFLP) methods, in order to differentiate between these breeds. Nucleotide sequencing of 337 bp MSTN gene detected five SNPs in Barki breed, two SNPs in Damascus breed, while the Zaraibi breed di...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Production and characterisation of exopolysaccharide from Streptomyces carpaticus isolated from marine sediments in Egypt and its effect on breast and colon cell lines
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Manal S. Selim, Shaimaa K. Amer, Sahar S. Mohamed, Marwa M. Mounier, Hala M. RifaatAbstractTwenty streptomycete strains were isolated from marine sediment samples collected from Nabq area, Sharm El-Sheikh, Red Sea Coast, Egypt. Four of them produce exopolysaccharides (EPS) showing marked in vitro antitumor activities. Morphological and cultural characteristics of the most significant strain (No. 3) were shown. Moreover, the sequence of this strain showed similarity with Streptomyces carpaticus. The results reve...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Healthcare-associated (HA) and community-associated (CA) methicillin resistant Staphylococcus aureus (MRSA) in Bangladesh – Source, diagnosis and treatment
This study reported source, diagnosis and treatment of HA-MRSA and CA-MRSA.A total of sixty-five clinical samples (urine, pus, wound swab) were collected from clinical origin of Dhaka city, Bangladesh. All the isolates were tested phenotypically by conventional methods and genotypically by PCR targeting nuc, pvl and mecA genes. Finally sequencing was carried out for pvl gene to know the mutagenic variation or any amino acid changes in pvl gene. Chi square test was employed for statistical analysis. Patients of age group 51–60 years are more susceptible (46.15%) to MRSA, CA-MRSA or HA-MRSA infection. Female are (32.30%)...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

In silico analysis of squalene synthase in Fabaceae family using bioinformatics tools
Publication date: Available online 6 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Zahra Aminfar, Masoud TohidfarAbstractTriterpenoid saponins are a diverse group of bioactive compounds, which are used for possessing of many biomedical and pharmaceutical products. Generally, squalene synthase (SQS) is defined as an emerging and essential branch point enzyme far from the major pathway of isoprenoids biosynthetic and a latent adjusting point, which manages carbon flux into triterpenes biosynthesis and sterols. The present study deals with the detailed characterization of SQS by bioinformatics ap...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Antibacterial activity of soil bacteria isolated from Kochi, India and their molecular identification
Publication date: Available online 14 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Davis Gislin, Dorairaj Sudarsanam, Gnanaprakasam Antony Raj, Kathirvelu BaskarAbstractThe present study, deal about the antibiosis activity of soil bacteria, isolated from 10 different locations of rhizosphere and diverse cultivation at Kochi, Kerala, India. The bacteria were isolated by standard serial dilution plate techniques. Morphological characterization of the isolate was done by Gram’s staining and found that all of them gram positive. Isolated bacteria were tested against 6 human pathogens viz., Esch...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Evaluation of the alleviative role of Chlorella vulgaris and Spirulina platensis extract against ovarian dysfunctions induced by monosodium glutamate in mice
In conclusion, Chlorella vulgaris and Spirulina platensis showed potential alleviative role against the monosodium glutamate ovarian dysfunction. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Improvement of cellulose degradation by cloning of endo-β-1, 3-1, 4 glucanase (bgls) gene from Bacillus subtilis BTN7A strain
Publication date: Available online 28 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Wafaa K. Hegazy, Mohamed S. Abdel-Salam, Azhar A. Hussain, Hoda H. Abo-Ghalia, Safa S. HafezAbstractThe aim of this study is to construct a new recombinant strain able to degrade cellulose efficiently. The endo-β-1, 3-1, 4 glucanase (bgls) gene was cloned from Bacillus subtilis BTN7A strain by using PCR technique. The specific primers of bgls gene were deduced. Optimization of PCR mixture and program were identified. The nucleotide sequence of bgls was placed in the public domain (GenBank accession number KM00...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Screening of plant growth promoting traits in heavy metals resistant bacteria: Prospects in phytoremediation
In this study, we isolated twenty seven (NF1-NF27) chromium resistant bacteria. The bacteria were tested for heavy metals (Cr, Zn, Cu, Ni, Pb and Co) resistance, Cr(VI) reduction and PGPB characters (phosphate solubilization, production of IAA and siderophores). The results showed that the bacterial isolates resist to heavy metals and reduce Cr(VI), with varying capabilities. 37.14% of the isolates have the capacity of solubilizing phosphate, 28.57% are able to produce siderophores and all isolates have the ability to produce IAA. Isolate NF2 that showed high heavy metal resistance and plant growth promotion characteristic...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research