Journal of Genetic Engineering and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Effect of vitamins and cell constructions on the activity of microbial fuel cell battery
Publication date: Available online 3 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Dena Z. Khater, K.M. El-Khatib, Rabeay Y.A. HassanAbstractConstruction of efficient performance of microbial fuel cells (MFCs) requires certain practical considerations. In the single chamber microbial fuel cell, there is no border between the anode and the cathode, thus the diffusion of the dissolved oxygen has a contrary effect on the anodic respiration and this leads to the inhibition of the direct electron transfer from the biofilm to the anodic surface. Here, a fed-batch single chambered microbial fuel cel...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Elevated carotenoids in staple crops: The biosynthesis, challenges and measures for target delivery
Publication date: Available online 6 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Adebanjo Ayobamidele BadejoAbstractPoverty eradication and global food security are among the targets of world leaders, most especially combating the scourge of hidden hunger. Provitamin A carotenoids cannot be synthesized de novo by human and so it must be taken as part of the diet. The deficiency of which is causing almost 6000 sights to be lost daily in most developing countries because of the monotonous starchy diets lacking substantial amount of carotenoid. Conventional breeding as well as genetic engineer...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants
Publication date: Available online 6 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Rania Samy Hanafy, Samia Ageeb AkladiousAbstractThis experiment assessed the biochemical changes in fenugreek plants exposed to gamma radiation. Two pot experiments were carried out during two growing seasons of 2015 and 2016. Seeds were subjected to five doses of gamma irradiation (25, 50, 100, 200 and 400 Gy) and were immediately planted into soil pots in a greenhouse. The experimental analysis was performed in M1 and M2 generations. Significant differences between irradiated and control plants were detecte...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Partial purification and characterization of exoinulinase produced from Bacillus sp.
In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was selected with 25.10 U/mL for further studies. The best inulinase producing colony was identified by partial 16S rRNA gene sequence as Bacillus sp. The crude inulinase was purified by using ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE – sephacel and obtained 1.9 purification fold with total activity 293 U. The purified enzyme was subjected to characterization studies and it was f...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Assessment of Ki-67 as a potential biomarker in patients with breast cancer
In conclusion; our study supports the finding that tissue Ki-67 expression may add prognostic information to that obtained from classical prognostic factors and can provide data of significant value to other important prognostic indicators such as pathological grading, and axillary lymph node involvement. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects
Publication date: Available online 19 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Saikat Gantait, Jitendriya PanigrahiAbstractAdhatoda vasica Nees, belonging to family Acanthaceae, is a well-known medicinal plant. It is endorsed for its pyrroloquinazoline alkaloids and its derivatives, such as vasicine and vasicinone. Germinating A. vasica seeds is a tedious task; on that account, vegetative propagation is the preferred method for its multiplication. For rapid and large-scale multiplication, germplasm conservation as well as secondary metabolites production, in vitro culture of A. vasica wa...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors
Publication date: Available online 21 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Ammar Mohammed Ahmed Ali, Mawahib ElAmin Mohamed El-Nour, Sakina Mohamed YagiAbstractThe present study was aimed at determining total phenolic and flavonoid contents and studying the antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome and callus, 6-gingerol and 6-shogaol and callus treated with elicitors. Petroleum ether (PE) and chloroform: methanol (1:1, v/v) (CM) extracts were prepared by maceration. Highest total phenolic content was obtained from the CM extract (60.34 ± 0.43 mg galli...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Screening of anti-inflammatory phytocompounds from Crateva adansonii leaf extracts and its validation by in silico modeling
Publication date: Available online 23 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Rathinavel Thirumalaisamy, Subramanian Ammashi, Govarthanan MuthusamyAbstractAnti-inflammatory phytocompounds from Crateva adansonii DC leaf extracts were identified by GCMS analysis and its anti-inflammatory potential was evaluated by in silico molecular docking study against inflammatory molecular targets. Three different (Aqueous, Methanol and Petroleum ether) dried leaf extracts of Crateva adansonii were obtained from soxhlet extraction method. Preliminary phytoconstituents analysis of three different leaf...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Screening of potential probiotic lactic acid bacteria and production of amylase and its partial purification
Publication date: Available online 27 March 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Tallapragada Padmavathi, Rayavarapu Bhargavi, Purushothama Rao Priyanka, Naige Ranganath Niranjan, Pogakul Veerabhadrappa PavitraAbstractProbiotics are the healthy living bacteria when administered in adequate amounts confers health benefits in the host. The main objective of present study was to screen the bacteria for potential probiotic characters and enzyme production. The probiotic characters like tolerance to low pH, bile salts, antibiotic sensitivity, hydrophobicity and auto-aggregation properties were ...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Immobilization of thermostable exo-inulinase from mutant thermophilic Aspergillus tamarii-U4 using kaolin clay and its application in inulin hydrolysis
In this study, attempts were made to immobilize purified exo-inulinase from mutant thermophic Aspergillus tamarii-U4 onto Kaolinite clay by covalent bonding cross-linked with glutaraldehyde with an immobilization yield of 66% achieved. The free and immobilized inulinases were then characterized and characterization of the enzymes revealed that temperature and pH optima for the activity of the free and immobilized enzymes were both 65 °C and pH 4.5 respectively. The free inulinase completely lost its activity after incubation at 65 °C for 6 h while the immobilized inulinase retained 16.4% of its activity under the sam...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Micropropagation protocol for Antigonon leptopus an important ornamental and medicinal plant
Publication date: Available online 5 April 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Zenna Fawzia Ghareeb, Lobna S. TahaAbstractThe effect of some factors on in vitro consecutive micropropagation behavior of Antigonon leptopus was examined including those of culture establishment, shootlets multiplication, rooting and acclimatization stages. The highest percent of aseptic cultures and survival of explants (100%) were obtained as a result of using Clorox 10% for 3 min followed by MC 0.1% for 2 min while, using each of them individually (Clorox 20% or MC 0.1%) for 5 min caused the highest p...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
High level expression and purification of recombinant flounder growth hormone in E. coli
Publication date: Available online 9 April 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Tae-Jin Choi, Temesgen Tola GeletuAbstractRecombinant flounder growth hormone was overproduced in E. coli by using codon optimized synthetic gene and optimized expression conditions for high level production. The gene was cloned into PET-28a expression vector and transformed into E. coli BL21 (DE3). Induction at lower temperature, lower IPTG concentrations and richer growth media during expression resulted in increased expression level. The protein expression profile was analyzed by SDS-PAGE, the authenticity w...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study
Publication date: Available online 13 April 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Ahyar Ahmad, Rosana Agus, Muh. Nasrum Massi, Rosdiana Natzir, Radha Madhyastha, Harish Kumar Madhyastha, Masugi MaruyamaAbstractThe appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development will stop the epidemic of TB. Some M. tuberculosis antigens, one of which is MPT83, ha...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Detection of myostatin gene MSTN in some goat breeds (Capra hircus)
Publication date: Available online 26 April 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Y.A. Dowidar, M.A. El-Sayed, Aly M. Elrefy, Hytham E. ShouraAbstractTill now not information about myostatin MSTN gene in Egyptian goat breeds. Here we show more information about MSTN in some Egyptian goat breeds to enrich the database with new sequences for Egyptian goat breeds. Our conducted study focused on detection and identifying the MSTN gene as a candidate gene of the muscles growth trait in three goat breeds (Zaraibi, Baladi and Damascus). We found the similarity between the registered sequences with...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent
Publication date: Available online 27 April 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kiptoo Geoffry, Rajeshwara N. AchurAbstractAmong different sources of lipases, fungal lipases have continued to attract a wide range of applications. Further, halophilic lipases are highly desirable for biodiesel production due to the need to mitigate environmental pollution caused as result of extensive use of fossil fuels. However, currently, the high production cost limits the industrial application of lipases. In order to address this issue, we have attempted to optimize lipase production by Fusarium solan...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
