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Synthesis of silver nanoparticles by Bacillus clausii and computational profiling of nitrate reductase enzyme involved in production
Publication date: Available online 28 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Koel Mukherjee, Rashmi Gupta, Gourav Kumar, Sarita Kumari, Saptaswa Biswas, Padmini PadmanabhanAbstractBiogenic synthesis of silver nanoparticles using microorganisms has found interest recently since last decade because of their prospect to synthesize nanoparticles of various size, shape and morphology which are eco-friendly. Here, an eco-friendly method for production of silver nanoparticles from Bacillus clausii cultured from Enterogermina is explored. Along with the biosynthesis and conformity test, in sili...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Phylogenetic diversity and biotechnological potentials of marine bacteria from continental slope of eastern Arabian Sea
Publication date: Available online 29 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Arakkaveettil Kabeer Farha, Thasneem TR, Aswathy Purushothaman, Jaseetha Abdul Salam, Abdulla Mohamed HathaAbstractMarine environments are substantially untapped source for the isolation of bacteria with the capacity to produce various extracellular hydrolytic enzymes, which have important ecological roles and promising biotechnological applications. Hydrolases constitute a class of enzymes widely distributed in nature from bacteria to higher eukaryotes. Marine microbial communities are highly diverse and have ...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Expression, purification and biological characterisation of recombinant human irisin (12.5 kDa)
Publication date: Available online 2 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kalpana Panati, Venkata Ramireddy Narala, Vydyanath R. Narasimha, Madhavi Derangula, Venkat R.R. Arva Tatireddigari, Suneetha YeguvapalliAbstractFibronectin type III domain containing 5 (FNDC5) is a transmembrane protein. Upon cleavage, it yields a peptide called irisin that is supposedly bind to an unknown receptor and facilitates browning of white adipose tissue (WAT). Increased levels of irisin are associated with increased levels of energy expenditure markers PGC-1α, UCP-1, besides abundance of beige adipoc...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India
Publication date: Available online 3 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): S. Muthukrishnan, T. Senthil Kumar, A. Gangaprasad, F. Maggi, M.V. RaoAbstractCeropegia thwaitesii Hook (Asclepiadaceae), an endemic plant species, due to habitat destruction and over exploitation has a very restricted distribution in the Western Ghats of Tamil Nadu, India. The present wrok aimed to determine the chemical composition, the total phenolic (TPC), flavonoid (TFC) and tannin content (TEC), and to assess the antioxidant properties of various extracts of in vivo plants (IVP) and in vitro regenerated pl...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system
This study was to investigate the variation effect of growth regulators and immersion frequency on production of biomass and flavonoid contained of G. procumbens shoots culture in temporary immersion bioreactor. Stem nodes were used as an explants and induction of shoots were done in solid MS medium supplemented with many kinds of growth regulator. The best treatments were used to produce biomass and flavonoid compounds in temporary immersion bioreactor; there are combination of IAA 2 mg/L and BA 4, 6, 8 mg/L and immersion frequency (5 min each 3 h; 15 min each 12 h). Results showed that the growths of G. procumb...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

In silico structural homology modeling of nif A protein of rhizobial strains in selective legume plants
Publication date: Available online 7 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Sadam D.V. Satyanarayana, M.S.R. Krishna, Pindi Pavan Kumar, Sirisha JeereddyAbstractSymbiosis is a complex genetic regulatory biological evolution which is highly specific pertaining to plant species and microbial strains. Biological nitrogen fixation in legumes is a functional combination of nodulation by nod genes and regulation by nif, fix genes. Three rhizobial strains (Rhizobium leguminosarum, Bradyrhizobium japonicum, and Mesorhizobium ciceri) that we considered for in silico analysis of nif A are proved ...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

Quorum sensing intervened bacterial signaling: Pursuit of its cognizance and repression
Publication date: Available online 7 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kayeen Vadakkan, Abbas Alam Choudhury, Ramya Gunasekaran, Janarthanam Hemapriya, Selvaraj VijayanandAbstractBacteria communicate within a system by means of a density dependent mechanism known as quorum sensing which regulate the metabolic and behavioral activities of a bacterial community. This sort of interaction occurs through a dialect of chemical signals called as autoinducers synthesized by bacteria. Bacterial quorum sensing occurs through various complex pathways depending upon specious diversity. Therefo...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research

In silico structural homology modeling of nif A protein of rhizobial strains in selective legume plants
Publication date: Available online 7 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Sadam D.V. Satyanarayana, M.S.R. Krishna, Pindi Pavan Kumar, Sirisha JeereddyAbstractSymbiosis is a complex genetic regulatory biological evolution which is highly specific pertaining to plant species and microbial strains. Biological nitrogen fixation in legumes is a functional combination of nodulation by nod genes and regulation by nif, fix genes. Three rhizobial strains (Rhizobium leguminosarum, Bradyrhizobium japonicum, and Mesorhizobium ciceri) that we considered for in silico analysis of nif A are proved ...
Source: Journal of Genetic Engineering and Biotechnology - July 8, 2018 Category: Biotechnology Source Type: research

Isolation, characterization, optimization, immobilization and batch fermentation of bioflocculant produced by Bacillus aryabhattai strain PSK1
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Ayat E. Abd El-Salam, Desouky Abd-El-Haleem, Amany S. Youssef, Sahar Zaki, Gadallah Abu-Elreesh, Samy A. El-AssarAbstractAmong others, isolate PSK1 was selected and identified by 16 S rDNA sequencing as Bacillus aryabhattai. Growth optimization of PSK1 and physicochemical parameters affected bioflocculant production was carried out by Plackett-Burman design and resulted in increasing in the activity by 4.5%. Bioflocculant production by entrapped and adsorbed immobilized microbial cells was performed us...
Source: Journal of Genetic Engineering and Biotechnology - July 7, 2018 Category: Biotechnology Source Type: research

Quorum sensing intervened bacterial signaling: Pursuit of its cognizance and repression
Publication date: Available online 7 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kayeen Vadakkan, Abbas Alam Choudhury, Ramya Gunasekaran, Janarthanam Hemapriya, Selvaraj VijayanandAbstractBacteria communicate within a system by means of a density dependent mechanism known as quorum sensing which regulate the metabolic and behavioral activities of a bacterial community. This sort of interaction occurs through a dialect of chemical signals called as autoinducers synthesized by bacteria. Bacterial quorum sensing occurs through various complex pathways depending upon specious diversity. Therefo...
Source: Journal of Genetic Engineering and Biotechnology - July 7, 2018 Category: Biotechnology Source Type: research

Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system
This study was to investigate the variation effect of growth regulators and immersion frequency on production of biomass and flavonoid contained of G. procumbens shoots culture in temporary immersion bioreactor. Stem nodes were used as an explants and induction of shoots were done in solid MS medium supplemented with many kinds of growth regulator. The best treatments were used to produce biomass and flavonoid compounds in temporary immersion bioreactor; there are combination of IAA 2 mg/L and BA 4, 6, 8 mg/L and immersion frequency (5 min each 3 h; 15 min each 12 h). Results showed that the growths of G. procumb...
Source: Journal of Genetic Engineering and Biotechnology - July 6, 2018 Category: Biotechnology Source Type: research

First study of sperm mediated gene transfer in Egyptian river buffalo
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Mohamed S. Hassanane, Aida I. El Makawy, Sahar M. Helalia, Ahmed S. Abdoon, Kamal M.A. Khalil, Tharwat A. Ghanem, Amany M. Tohamy, Xiao-Feng Sun, Wei ShenAbstractThe present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfec...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Mitochondrial DNA genetic variations among four horse populations in Egypt
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Othman E. Othman, Karima F. Mahrous, Heba I. ShafeyAbstractHorses are one of the early domesticated animals in the world that changed societies and civilizations on a continent-wide scale. Due to the rare information about the genetic characterization of different horse populations in Egypt, this study aimed to identify the genetic biodiversity and relationships between four horse populations reared in Egypt. Genomic DNA was extracted and mtDNA region was amplified using polymerase chain reaction (PCR). The...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Purification and characterization of deoxyribonuclease from small intestine of camel Camelus dromedarius
In conclusion, the characterization of DNase 3 indicated that the enzyme is considered as a member of DNase I family. The low Km value of the DNA suggested that the high digestion of DNA of camel forage by small intestine DNase 3. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Th2 related markers in milk allergic inflammatory mice model, versus OVA
In conclusion, this study suggests that the low fat milk-induced inflammation showed hallmarks of allergic airway inflammatory model such as eosinophilic influx in BALF, increased numbers of Th2 cells, augmented expression of IL-13, elevated levels of circulatory IgG1 and IgE, signs of robust pulmonary inflammation, and most importantly it is a cheap and promising model for studying acute allergic airway inflammation and acute asthma. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research