Journal of Genetic Engineering and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells
This study aims to evaluate the pulirpotency and the ability of Muse cells to generate insulin producing cells (IPCs) after in vitro differentiation protocol compared to the non-Muse cells. Muse cells were isolated by FACSAria III cell sorter from adipose-derived MSCs and were evaluated for its pluripotency. Following in vitro differentiation, IPCs derived from Muse and non-Muse cells were evaluated for insulin production. Muse cells comprised 3.2 ± 0.7% of MSCs, approximately 82% of Muse cells were positive for anti stage-specific embryonic antigen-3 (SSEA-3). Pluripotent markers were highly expressed in Muse versus ...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Development and evaluation of latex agglutination test coating with recombinant antigen, LipL32 for serodiagnosis of human leptospirosis
Publication date: Available online 3 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kotchakorn Thongsukkaeng, Rerngwit BoonyomAbstractLeptospirosis is a widespread zoonotic disease caused by Leptospira interrogans. Symptoms of disease range from mild symptoms to serious complications including, jaundice, pulmonary hemorrhage, renal and hepatic failure, which may prove fatal. Clinical presentations of this disease are similar with other febrile illness. Therefore, rapid and appropriated laboratory diagnostic tests are needed to aid clinical case identification. As these reasons, objective of...
Source: Journal of Genetic Engineering and Biotechnology - December 4, 2018 Category: Biotechnology Source Type: research
Callus mediated shoot organogenesis and regeneration of cytologically stable plants of Ledebouria revoluta: An ethnomedicinal plant with promising antimicrobial potency
Publication date: Available online 12 November 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Sk Moquammel Haque, Avijit Chakraborty, Biswajit GhoshAbstractLedebouria revoluta are important ethnomedicinal plant found in India and South Africa. Micropropagation via indirect shoot organogenesis had been established from three types of explant (i.e. scale leaf, leaf lamina and root) of L. revoluta. Scale leaf was found superior as compared to leaf lamina and root explant with respect to their organogenic callus induction potentiality. Murashige and Skoog (1962) [MS] media supplemented with 3.0 mg L...
Source: Journal of Genetic Engineering and Biotechnology - November 12, 2018 Category: Biotechnology Source Type: research
Population structure, morphological and genetic diversity within and among melon (Cucumis melo L.) landraces in Iran
Publication date: Available online 28 August 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Masoud Maleki, Abdolali Shojaeiyan, Sajad Rashidi MonfaredAbstractISSR markers were applied to evaluate the genetic diversity and differentiation of 270 individuals of 27 Iranian C. melo landraces of various varietal groups include vars. inodorous, cantalupensis, reticulatus, ameri, dudaim. Genetic diversity among the studied genotypes obtained by GeneAlex analysis (H = 0.08, I = 0.12, Na = 0.77, PPL = 22.6%). Cluster analysis divided Iranian melon landraces into two main cluster. Non-sweet ge...
Source: Journal of Genetic Engineering and Biotechnology - August 29, 2018 Category: Biotechnology Source Type: research
Feline panleukopenia viral infection in cats: Application of some molecular methods used for its diagnosis
Publication date: Available online 27 August 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Romane A. Awad, Wagdy K.B. Khalil, Ashraf G. AttallahAbstractForty diseased cats and seven healthy control cats from different sex, ages and breeds had examined clinically to confirm presence or absence of clinical symptoms of Feline panleukopenia disease (FP). Several tools including ELISA, gene expression analysis (qRT-PCR), DNA fragmentation test and apoptosis assay were conducted to determine the Feline panleukopenia disease in cat tissues. Clinical symptoms in the form of depression, fever, anorexia, vom...
Source: Journal of Genetic Engineering and Biotechnology - August 28, 2018 Category: Biotechnology Source Type: research
Buffalo species identification and delineation using genetic barcoding markers
In this study, COI barcode sequence in seventy one river buffalo samples were determined, analyzed and deposited in Genbank barcode database and barcode of life database (BOLD) to contribute for construction of public reference library for COI barcode sequence in river buffalo. Moreover COI barcode sequence was used to identify the closely related buffalo groups: river buffalo, swamp buffalo, lowland anoa and African buffalo. Results indicated the success of the COI barcode in the identification of each of the tested groups. Whereas a suggested sequence of other mitochondrial segment representing two successive transfer RN...
Source: Journal of Genetic Engineering and Biotechnology - August 10, 2018 Category: Biotechnology Source Type: research
Cytogenetic effects of silver and gold nanoparticles on Allium cepa roots
In this study, the root cells of Allium cepa were treated with both gold and silver nanoparticles of different concentrations (1 mg/L, 5 mg/L and 10 mg/L) along with control for 72 h. Experimental results revealed that after 72 h of exposure, a significant decrease in mitotic index (MI) from 68% (control) to 52.4% (1 mg/L), 47.3% (5 mg/L) and 41.4% (10 mg/L) for gold nanoparticles and 57.1% (1 mg/L), 53% (5 mg/l), 55.8% (10 mg/L) for silver nanoparticles. Through minute observation of the photograph, it was recorded that some specific chromosomal abnormalities such as stickiness of chromosome, chromos...
Source: Journal of Genetic Engineering and Biotechnology - August 4, 2018 Category: Biotechnology Source Type: research
Valorisation of chicken feathers for xanthan gum production using Xanthomonas campestris MO-03
In this study, xanthan production was investigated using a local isolate of Xanthomonas campestris MO-03 in medium containing various concentrations of chicken feather peptone (CFP) as an enhancer substrate. CFP was produced with a chemical process and its chemical composition was determined. The addition of CFP (1–8 g/l) increased the conversion of sugar to xanthan gum in comparison with the control medium, which did not contain additional supplements. The highest xanthan production (24.45 g/l) was found at the 6 g/l CFP containing control medium in 54 h. This value was 1.73 fold higher than that of control medi...
Source: Journal of Genetic Engineering and Biotechnology - July 25, 2018 Category: Biotechnology Source Type: research
Biolytic extraction of poly(3-hydroxybutyrate) from Bacillus megaterium Ti3 using the lytic enzyme of Streptomyces albus Tia1
Publication date: Available online 21 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Neetu Israni, Surabhi Thapa, Srividya ShivakumarAbstractThe applicability of Streptomyces sp. cell lytic enzymes for devising a simple and competent biological polyhydroxyalkanoate (PHA) recovery approach from Bacillus megaterium cells was investigated. B. megaterium strain Ti3 produced 50% (w/w) PHA using glucose as carbon source. The intracellular PHA was recovered employing a non-PHA accumulating actinomycetes (Tia1) identified as Streptomyces albus, having potent lytic activity against living and heat inact...
Source: Journal of Genetic Engineering and Biotechnology - July 21, 2018 Category: Biotechnology Source Type: research
Purification and characterization of alkaline soda-bleach stable protease from Bacillus sp. APP-07 isolated from Laundromat soil
In conclusion, the remarkable tolerance and stability of the enzyme explored the promising candidature for the several potential applications in the laundry detergents. The sustainability of the enzyme might serve several possible applications in the laundry detergents, leather industries, and other harsh industrial processes. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 20, 2018 Category: Biotechnology Source Type: research
Immobilized lipase from Lactobacillus plantarum in meat degradation and synthesis of flavor esters
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Sita Ramyasree Uppada, Mahesh Akula, Anupam Bhattacharya, Jayati Ray DuttaAbstractMicrobial lipases owing to their broad substrate specificity are widely used in various industrial applications like food processing, organic synthesis, detergent formulation and oil manufacturing. In the current study the immobilized lipase from Lactobacillus plantarum was found novel in degrading meat which can be applied in medical field and also in synthesizing different short chain fatty acid esters like 2,3,4-hydroxybenz...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv. ‘Barhee’
Publication date: Available online 10 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Ahmed Madi Waheed Al-Mayahi, Abdulminam Hussien Ali, Hussein J. ShareefAbstractMass propagation of date palm through indirect somatic embryogenesis or organogenesis has attracted the interest of commercial producers. But, this technique still faces some problems that hindered the production of date palm plantlets in vitro. Tissue browning is one of the serious problems that reduce callus growth and shoot regeneration. So the objective of the present study is to investigate the effect of cold pretreatment on cal...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Th2 related markers in milk allergic inflammatory mice model, versus OVA
In conclusion, this study suggests that the low fat milk-induced inflammation showed hallmarks of allergic airway inflammatory model such as eosinophilic influx in BALF, increased numbers of Th2 cells, augmented expression of IL-13, elevated levels of circulatory IgG1 and IgE, signs of robust pulmonary inflammation, and most importantly it is a cheap and promising model for studying acute allergic airway inflammation and acute asthma. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Effect of natural PAL-enzyme on the quality of egg white and mushroom flour and study its impact on the expression of PKU related genes and phenylalanine reduction in mice fed on
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Hesham A. Eissa, Zeinab Y. Abdallah, Wagdy K.B. Khalil, Wafaa A. Ibrahim, Hoda F. Booles, Mahrousa M. HassananeAbstractPKU patients react to therapy with a low phenylalanine diet, but adherence to this diet is troublesome, subsequently the expansion of alternative ways is demand. Phenylalanine ammonia lyase (PAL) is one of this ways, which converts phenylalanine to harmless metabolites; trans-cinnamic acid and ammonia. In the current study, the extraction of PAL enzyme was used to investigate the efficiency...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
New polymorphism in the 5′ flanking region of IGF-1 gene and its association with wool traits in Egyptian Barki sheep
This study adds evidence of the association between IGF-1 gene polymorphism and CFW of wool in Egyptian Barki sheep. Therefore; it is important to consider IGF-1 gene as a candidate gene marker for wool weight traits and it should be identified before using successful breeding program. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
