Journal of Genetic Engineering and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Assessment of genetic diversity in Salvadora persica L. based on inter simple sequence repeat (ISSR) genetic marker
In this study, ISSR markers were used to establish the level of genetic relationships and polymorphism 50 genotypes of Salvadora persica collected from 6 different regions of Hormozgan province. The ISSR analysis with 9 anchored primers also generated 105 scorable loci, of which 85 were polymorphic (80.95%). Parameters of genetic diversity and its partitioning were calculated. The genetic analysis demonstrated that S. persica maintain relatively high genetic diversity (PIC was 0.63, Na was 1.27 and Ho and He were 0.15 and 0.17 respectively). The coefficient of genetic differentiation among populations based on FST equaled ...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Study on the potential of cold-active lipases from psychrotrophic fungi for detergent formulation
Publication date: Available online 7 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Sanjay Sahay, Deepak ChouhanAbstractLipases from psychrotrophic fungal isolates BPF4 and BPF6 identified as Penicilium canesense and Pseudogymnoascus roseus respectively were characterized for their compatibility towards laundry detergent. BPF4 and BPF6 lipases showed maximum activity at pH 11 and 9 respectively and at 40 °C. The residual activities at 20 °C and 4 °C of BPF4 lipase were 35% and 20% and of BPF6 lipase were 70% and 20 °C respectively. Both the enzymes were stable at 4 °C, 20 °C an...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Isolation and characterization of Bacillus sp. strain BC01 from soil displaying potent antagonistic activity against plant and fish pathogenic fungi and bacteria
Publication date: Available online 7 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Md Javed Foysal, Asura Khanam LisaAbstractFungal and bacterial pathogens infect a diverse range of hosts including various plant and animal species. Fungal and bacterial diseases, especially of plants and aquatic animals, such as fish, lead to significant damage to crops and aquaculture, respectively, worldwide. The present study was conducted to isolate and characterize potent Bacillus strains with significant antagonistic activity against the major plant and fish pathogenic fungi and bacteria. We randomly colle...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Molecular diversity of internal transcribed spacer among the monoconidial isolates of Magnaporthe oryzae isolated from rice in Southern Karnataka, India
Publication date: Available online 19 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): D. Jagadeesh, M.K. Prasanna Kumar, R. Chandrakanth, N.S. DevakiAbstractBlast disease of rice plant is caused by Magnaporthe oryzae (anamorph Pyricularia oryzae). This disease is recognized to be one of the most serious diseases of rice crop around the world. A total of 72 monoconidial isolates of M. oryzae obtained from blast disease samples collected around Southern Karnataka were characterized using internal transcribed spacers of the ribosomal DNA sequences. These were analyzed by comparing with already depos...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Enhancement of nematicidal potential through cloning and expression of chitinase gene from Bacillus subtilis subsp. Subtilis BTN7A strain
Publication date: Available online 23 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Mohamed S. Abdel-Salam, Hoda H. Ameen, Abdallah S.M. Kassab, Ahmed E.A. Mahgoob, Usama S. ElkelanyAbstractA gene encoding chitinase from B. subtilis has been isolated after optimization of PCR conditions. It was cloned with two different prometers, T7 promoter of the pJET1.2/blunt cloning vector and the SP6 promoter of pGEM®-T Easy vector. After transforming E. coli DH5α, two transformants were selected, CHI-NRC-4 from the first vector and T-CHI-NRC-6 from the second vector, and used for further studies. The c...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Purification and characterization of alkaline protease with novel properties from Bacillus cereus strain S8
Publication date: Available online 26 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): B.K.M Lakshmi, D. Muni Kumar, K.P.J HemalathaAbstractProteases are the hydrolytic enzymes which hydrolyzes peptide bond between proteins with paramount applications in pharmaceutical and industrial sector. Therefore production of proteases with efficient characteristics of biotechnological interest from novel strain is significant. Hence, in this study, an alkaline serine protease produced by Bacillus cereus strain S8 (MTCC NO 11901) was purified and characterized. The alkaline protease was purified by ammonium ...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Biodegradation of feather waste by keratinase produced from newly isolated Bacillus licheniformis ALW1
Publication date: Available online 28 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Azza M. Abdel-Fattah, Mamdouh S. El-Gamal, Siham A. Ismail, Mohamed A. Emran, Amal M. HashemAbstractKeratinase are proteolytic enzymes which have gained much attention to convert keratinous wastes that cause huge environmental pollution problems. Ten microbial isolates were screened for their keratinase production. The most potent isolate produce 25.2 U/ml under static condition and was primarily identified by partial 16s rRNA gene sequence as Bacillus licheniformis ALW1. Optimization studies for the fermentat...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Increased level of B cell differentiation factor in systemic lupus erythematosus patients
ConclusionBCDF and IgM are increased in SLE patients and are promissing diagnostic markers for SLE. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
In silico studies on bacterial xylanase enzyme: Structural and functional insight
Publication date: Available online 31 May 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Bhramar Dutta, Aparna Banerjee, Priyanka Chakraborty, Rajib BandopadhyayAbstractXylans are the second most abundant form of hemicelluloses and are the second most abundant polysaccharide in nature after cellulose. To degrade xylan, microbes produce mainly xylanase enzyme. Wide range of microorganisms like fungi, bacteria, yeast, marine algae etc. are capable of producing xylanase. Main source of xylanase is fungi but industrial production of bacterial xylanase is low cost, easy downstream process and high produc...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Detecting protein complexes based on a combination of topological and biological properties in protein-protein interaction network
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Pooja Sharma, D.K. Bhattacharyya, J.K. KalitaAbstractProtein complexes are known to play a major role in controlling cellular activity in a living being. Identifying complexes from raw protein protein interactions (PPIs) is an important area of research. Earlier work has been limited mostly to yeast. Such protein complex identification methods, when applied to large human PPIs often give poor performance. We introduce a novel method called CSC to detect protein complexes. The method is evaluated in terms of pos...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Production and purification of IgY antibodies from chicken egg yolk
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Wala Ahmad Amro, Wael Al-Qaisi, Fawzi Al-RazemAbstractThe isolation of polyclonal antibodies from the serum of immunized mammals has significantly contributed to scientific research and diagnosis. The fact that recent technologies allow the production of antibodies in the yolk of eggs laid by hens, has led to the development of an alternative method for antibody generation that is less stressful to animals. As hens are kept under almost all their natural conditions, antibodies are isolated from the collected eg...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Biosynthesis of silver nanoparticles formation from Caesalpinia pulcherrima stem metabolites and their broad spectrum biological activities
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Pooja Moteriya, Sumitra ChandaAbstractThe present work illustrates eco-friendly, rapid and cost effective method of AgNPs synthesis using C. pulcherrima stem extract. Initially, various physico chemical factors were optimized. Characterization was done by different spectroscopic and microscopic analysis. AgNPs were spherical in shape with an average size of 8 nm. AgNPs showed good synergistic antimicrobial, antibiofilm and antioxidant activity. The cytotoxicity effect against HeLa cancer cell line was dose de...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Green synthesis of zero valent colloidal nanosilver targeting A549 lung cancer cell: In vitro cytotoxicity
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Minakshi Jha, Navinchandra G. ShimpiAbstractAn eco-friendly green approach was proposed to synthesise stable, cytotoxic colloidal silver nanoparticles (AgNPs) using Momordica charantia (M. charantia) fruit extract. Bioinspired green method adopted for fabrication of AgNPs because of easy, fast, low-cost and benign bioprocess. Phytocomponents played the crucial role in capping, stabilisation and inherent cytotoxic potential of colloidal nanosilver. The physiochemical, crystalline, optical and morphological prope...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Study the influence of culture conditions on rennin production by Rhizomucor miehei using solid-state fermentations
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Houthail Alahmad Aljammas, Hassan Al Fathi, Walid AlkhalafAbstractInvestigations were conducted on the production of Rennin enzyme from the fungi Rhizomucor miehei 3420 NRRL using Solid-State fermentation. Wheat bran was used as a substrate. The influence of moisture content, incubation temperature, and the initial pH of fermentation medium were studied. The protein content, milk clotting activity (MCA), specific activity, proteolytic activity (PA), and (MCA/PA) ratio of the extracted enzyme were calculated aft...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
Overexpression of rice thaumatin-like protein (Ostlp) gene in transgenic cassava results in enhanced tolerance to Colletotrichum gloeosporioides f. sp. manihotis
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Patroba Odeny Ojola, Evans N. Nyaboga, Paul N. Njiru, George OrindaAbstractCassava (Manihot esculenta Crantz) is the most important staple food for more than 300 million people in Africa, and anthracnose disease caused by Colletotrichum gloeosporioides f. sp. manihotis is the most destructive fungal disease affecting cassava production in sub-Saharan Africa. The main objective of this study was to improve anthracnose resistance in cassava through genetic engineering. Transgenic cassava plants harbouring rice ...
Source: Journal of Genetic Engineering and Biotechnology - July 11, 2018 Category: Biotechnology Source Type: research
