Journal of Genetic Engineering and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Decolorization of textile reactive dyes by bacterial monoculture and consortium screened from textile dying effluent
In this study, five dye decolorizing bacteria were isolated from dyeing effluent using selective enrichment culture in Bushnell-Haas (BH) medium amended with co-substrate (i.e. glucose, yeast extract) and 100 mg L−1 of each commercially available reactive dyes viz. Novacron Orange FN-R, Novacron Brilliant Blue FN-R, Novacron Super Black G, Bezema Yellow S8-G and Bezema Red S2-B. The isolated bacteria were identified and assigned as Neisseria sp., Vibrio sp., Bacillus sp., Bacillus sp. and Aeromonas sp. based on their phenotypic (cultural, morphological, physiological and biochemical characteristic) observation. The d...
Source: Journal of Genetic Engineering and Biotechnology - February 23, 2018 Category: Biotechnology Source Type: research

Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid
Publication date: Available online 22 February 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Edy Setiti Wida Utami, Sucipto Hariyanto, Yosephine Sri Wulan Manuhara A protocol for genetic transformation mediated by Agrobacterium tumefaciens and production of transgenic Dendrobium lasianthera has been developed for the first time. The 8-week-old protocorm explants were used as target of transformation with Agrobacterium tumefaciens strain LBA4404 carrying plasmid pG35SKNAT1. Several parameters such as infection period, Agrobacterium density, concentration of acetosyringone, and co-cultivation p...
Source: Journal of Genetic Engineering and Biotechnology - February 23, 2018 Category: Biotechnology Source Type: research

Kolaviron and selenium reduce hydrogen peroxide-induced alterations of the inflammatory response
Publication date: Available online 17 February 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Tebekeme Okoko The abilities of kolaviron and selenium (either separately or in combination) to prevent hydrogen peroxide-induced alterations in cell viability and activation were investigated. The cell line U937 was incubated with the antioxidants (i.e. kolaviron or selenium) for 24 h before exposure to hydrogen peroxide and cell viability was assessed via trypan blue dye exclusion assay. The U937 cells were also transformed to the macrophage form, incubated with the antioxidants before exposure to h...
Source: Journal of Genetic Engineering and Biotechnology - February 18, 2018 Category: Biotechnology Source Type: research

Scenedesmus obliquus: Antioxidant and antiviral activity of proteins hydrolyzed by three enzymes
Conclusion S. obliquus protein hydrolysates have a potential as antioxidative neutraceutical ingredients and a potential therapeutic agent against CVB3. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - February 12, 2018 Category: Biotechnology Source Type: research

Interaction of rs316019 variants of SLC22A2 with metformin and other drugs- an in silico analysis
In this study, we used computational approaches to investigate the effect of 270A > S change in SLC22A2 on interaction with metformin and other drugs. Based on the structural models, all substrates bind to the same pocket of SLC22A2. The substrates fit better to the binding site of 270A form of SLC22A2. The binding site has a few core interacting residues, among which SER358 appears to be the most important. It is an in silico prediction that the T2D patients, who are under metformin regimen, should be cautious in taking ranitidine (an over-the-counter sold drug) on a regular basis as it may lead to metformin as...
Source: Journal of Genetic Engineering and Biotechnology - February 12, 2018 Category: Biotechnology Source Type: research

Expression of Leptospira membrane proteins Signal Peptidase (SP) and Leptospira Endostatin like A (Len A) in BL-21(DE3) is toxic to the host cells
Publication date: Available online 2 February 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Padikara K. Satheeshkumar, Prasannan V. Anu, Mohmed I. Junaida, Madathiparambil G. Madanan, Tennison Jebasingh, Ananthakrishnan J. Nair, Gangaprasad A. Nair, Govinda Pillai M. Nair, Perumana R. Sudhakaran Heterologous expression of Integral Membrane Proteins (IMPs) is reported to be toxic to the host system in many studies. Even though there are reports on various concerns like transformation efficiency, growth properties, protein toxicity, inefficient expression and protein degradation in IMP ov...
Source: Journal of Genetic Engineering and Biotechnology - February 12, 2018 Category: Biotechnology Source Type: research

Optimization of quorum quenching mediated bacterial attenuation of Solanum torvum root extract by response surface modelling through Box-Behnken approach
Publication date: Available online 7 February 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Kayeen Vadakkan, Selvaraj Vijayanand, Abbas Alam Choudhury, Ramya Gunasekaran, Janarthanam Hemapriya The present study was intended to optimize the quorum sensing inhibitory action of Solanum torvum root extract against Chromobacterium violaceum. Factors such as bacterial density, frequency of administration and concentration of extract were analysed. Plant samples were collected from Thrissur District, Kerala, India. Response surface modelling of factors by Box-Behnken approach was employed for opti...
Source: Journal of Genetic Engineering and Biotechnology - February 12, 2018 Category: Biotechnology Source Type: research

Down-regulation of circulating microRNA let-7a in Egyptian smokers
In conclusion, plasma let-7a was significantly down-regulated in the smokers, and it might be considered a candidate biomarker to discriminate between smokers and non-smokers. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - February 12, 2018 Category: Biotechnology Source Type: research

Statistical optimization of crude oil bio-degradation by a local marine bacterium isolate Pseudomonas sp. sp48
Publication date: Available online 6 January 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Soha Farag, Nadia A. Soliman, Yasser R. Abdel-Fattah Pseudomonas sp. sp48, a marine bacterium isolated from Bahary area (Alexandria, Egypt), showed a high potency for oil degradation up to 1.5%. Additionally, it showed an ability to consume aromatic hydrocarbons (phenol & naphthalene) and aliphatic (pentadecane) reaching to 79; 73; 62%, respectively. In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the degradation potency. Analysis of Pl...
Source: Journal of Genetic Engineering and Biotechnology - January 7, 2018 Category: Biotechnology Source Type: research

Green synthesis of zero valent colloidal nanosilver targeting A549 lung cancer cell: In vitro cytotoxicity
Publication date: Available online 5 January 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Minakshi Jha, Navinchandra G. Shimpi, S.S. Sonawane An eco-friendly green approach was proposed to synthesise stable, cytotoxic colloidal silver nanoparticles (AgNPs) using Momordica charantia (M. charantia) fruit extract. Bioinspired green method adopted for fabrication of AgNPs because of easy, fast, low-cost and benign bioprocess. Phytocomponents played the crucial role in capping, stabilisation and inherent cytotoxic potential of colloidal nanosilver. The physiochemical, crystalline, optical and mor...
Source: Journal of Genetic Engineering and Biotechnology - January 5, 2018 Category: Biotechnology Source Type: research

Molecular detection and PCR-RFLP analysis using Pst1 and Alu1 of multidrug resistant Klebsiella pneumoniae causing urinary tract infection in women in the eastern part of Bangladesh
In this study, a rapid combined polymerase chain reaction and restriction fragment length polymorphism analysis was developed to identify K. pneumoniae in women, infected with urinary tract infection in the Sylhet city of Bangladesh. Analysis of 11 isolates from women at the age range of 20–55 from three different hospitals were done firstly by amplification with K. pneumoniae specific ITS primers. All of the 11 collected isolates were amplified in PCR and showed the expected 136 bp products. Then, restriction fragment length polymorphism analysis of 11 isolates were conducted after PCR amplification by 16s rRNA univer...
Source: Journal of Genetic Engineering and Biotechnology - January 4, 2018 Category: Biotechnology Source Type: research

Biosynthesis of silver nanoparticles formation from Caesalpinia pulcherrima stem metabolites and their broad spectrum biological activities
Publication date: Available online 4 January 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Pooja Moteriya, Sumitra Chanda The present work illustrates eco-friendly, rapid and cost effective method of AgNPs synthesis using C. pulcherrima stem extract. Initially, various physico chemical factors were optimized. Characterization was done by different spectroscopic and microscopic analysis. AgNPs were spherical in shape with an average size of 8 nm. AgNPs showed good synergistic antimicrobial, antibiofilm and antioxidant activity. The cytotoxicity effect against HeLa cancer cell line was dose de...
Source: Journal of Genetic Engineering and Biotechnology - January 4, 2018 Category: Biotechnology Source Type: research

Augmented cellulase production by Bacillus subtilis strain MU S1 using different statistical experimental designs
Publication date: Available online 4 January 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): C.P. Sreena, Denoj Sebastian The production of cellulase by Bacillus subtilis MU S1, a strain isolated from Eravikulam National Park, was optimized using one-factor-at-a-time (OFAT) and statistical methods. Physical parameters like incubation temperature and agitation speed were optimized using OFAT and found to be 40 °C and 150 rpm, respectively, whereas, medium was optimized by statistical tools. Plackett-Burman design (PBD) was employed to screen the significant variables that highly influence ce...
Source: Journal of Genetic Engineering and Biotechnology - January 4, 2018 Category: Biotechnology Source Type: research

Overexpression of rice thaumatin-like protein (Ostlp) gene in transgenic cassava results in enhanced tolerance to Colletotrichum gloeosporioides f. sp. manihotis
Publication date: Available online 3 January 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Patroba Odeny Ojola, Evans N. Nyaboga, Paul N. Njiru, George Orinda Cassava (Manihot esculenta Crantz) is the most important staple food for more than 300 million people in Africa, and anthracnose disease caused by Colletotrichum gloeosporioides f. sp. manihotis is the most destructive fungal disease affecting cassava production in sub-Saharan Africa. The main objective of this study was to improve anthracnose resistance in cassava through genetic engineering. Transgenic cassava plants harbouring ric...
Source: Journal of Genetic Engineering and Biotechnology - January 3, 2018 Category: Biotechnology Source Type: research

Influence of bioprocess variables on the production of extracellular chitinase under submerged fermentation by Streptomyces pratensis strain KLSL55
Publication date: Available online 3 January 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): A. Shivalee, K. Lingappa, Divatar Mahesh Chitinases are the enzymes which are capable of hydrolyzing chitin to its monomer N-acetyl glucosamine (GlcNac). Present study emphasizes on the impact of critical process variables on the production of chitinase from Streptomyces pratensis strain KLSL55. Initially the isolate was noticed to produce 84.67 IU chitinase in basal production medium. At optimization of bioprocess variables, the physical parameters pH of 8.00, 40 °C of incubation temperature, agit...
Source: Journal of Genetic Engineering and Biotechnology - January 3, 2018 Category: Biotechnology Source Type: research