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A review on advanced methods in plant gene targeting
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Raghavendrarao Sanagala, Anil Kumar Moola, Ranjitha Kumari Bollipo DianaAbstractPlant genetic engineering is one of the most significant tools implemented in the modern molecular crop breeding techniques. The conventional approaches of plant genetic transformation include Agrobacterium tumefaciens, particle bombardment, DNA uptake into protoplast. The transgenic events derived by these methods carry the transgenes that are integrated at random sites in the plant genome. Novel techniques that mediate integra...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Micropropagation of carnation (Dianthus caryophyllus L.) in liquid medium by temporary immersion bioreactor in comparison with solid culture
We report a comparison between the results of experiments aimed at improving carnation micropropagation using new bioreactor according to Temporary Immersion Bioreactor (TIB) and solid culture. By applying different levels of BAP, at the concentration of 3 mg L−1, we observed 14.3 new shoots in TIB, but the number of new shoots on solid medium reached to 5.7 at the same treatment. Our results also showed that with 3 mg L−1 BAP in TIB, the initial fresh weight of plant material increased from 10 g to 450 g after 15 days. It is concluded that TIB showed more than 10 times shoot producti...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Cytotoxic and molecular impacts of allelopathic effects of leaf residues of Eucalyptus globulus on soybean (Glycine max)
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Hala M. Abdelmigid, Maissa M. MorsiAbstractEucalyptus trees litter plays a crucial role in structuring plant populations and regulating crop quality. To help characterize the allelopathic impact of Eucalyptus plantations and understand the interactions between tree litter and understorey plant populations, we performed two different genomic approaches to determine soybean (Glycine max) crop plant response to biotic stress induced by leaf residues of Eucalyptus globulus trees. For assessing cell death, a qua...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Serum apelin levels and metabolic risk markers in obese women
ConclusionThe present study suggests the significant role that might be mediated by apelin for developing abnormal metabolic parameters among Egyptian obese women. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Influence of bioprocess variables on the production of extracellular chitinase under submerged fermentation by Streptomyces pratensis strain KLSL55
Publication date: Available online 3 January 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): A. Shivalee, K. Lingappa, Divatar MaheshAbstractChitinases are the enzymes which are capable of hydrolyzing chitin to its monomer N-acetyl glucosamine (GlcNac). Present study emphasizes on the impact of critical process variables on the production of chitinase from Streptomyces pratensis strain KLSL55. Initially the isolate was noticed to produce 84.67 IU chitinase in basal production medium. At optimization of bioprocess variables, the physical parameters pH of 8.00, 40 °C of incubation temperature, agi...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Statistical optimization of crude oil bio-degradation by a local marine bacterium isolate Pseudomonas sp. sp48
Publication date: Available online 6 January 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Soha Farag, Nadia A. Soliman, Yasser R. Abdel-FattahAbstractPseudomonas sp. sp48, a marine bacterium isolated from Bahary area (Alexandria, Egypt), showed a high potency for oil degradation up to 1.5%. Additionally, it showed an ability to consume aromatic hydrocarbons (phenol & naphthalene) and aliphatic (pentadecane) reaching to 79; 73; 62%, respectively. In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the degradation potency. Analysis of Plackett-...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Interaction of rs316019 variants of SLC22A2 with metformin and other drugs- an in silico analysis
In this study, we used computational approaches to investigate the effect of 270A > S change in SLC22A2 on interaction with metformin and other drugs. Based on the structural models, all substrates bind to the same pocket of SLC22A2. The substrates fit better to the binding site of 270A form of SLC22A2. The binding site has a few core interacting residues, among which SER358 appears to be the most important. It is an in silico prediction that the T2D patients, who are under metformin regimen, should be cautious in taking ranitidine (an over-the-counter sold drug) on a regular basis as it may lead to metformin associate...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Scenedesmus obliquus: Antioxidant and antiviral activity of proteins hydrolyzed by three enzymes
ConclusionS. obliquus protein hydrolysates have a potential as antioxidative neutraceutical ingredients and a potential therapeutic agent against CVB3. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Expression of Leptospira membrane proteins Signal Peptidase (SP) and Leptospira Endostatin like A (Len A) in BL-21(DE3) is toxic to the host cells
Publication date: Available online 2 February 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Padikara K. Satheeshkumar, Prasannan V. Anu, Mohmed I. Junaida, Madathiparambil G. Madanan, Tennison Jebasingh, Ananthakrishnan J. Nair, Gangaprasad A. Nair, Govinda Pillai M. Nair, Perumana R. SudhakaranAbstractHeterologous expression of Integral Membrane Proteins (IMPs) is reported to be toxic to the host system in many studies. Even though there are reports on various concerns like transformation efficiency, growth properties, protein toxicity, inefficient expression and protein degradation in IMP overexp...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Optimization of quorum quenching mediated bacterial attenuation of Solanum torvum root extract by response surface modelling through Box-Behnken approach
Publication date: Available online 7 February 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kayeen Vadakkan, Selvaraj Vijayanand, Abbas Alam Choudhury, Ramya Gunasekaran, Janarthanam HemapriyaAbstractThe present study was intended to optimize the quorum sensing inhibitory action of Solanum torvum root extract against Chromobacterium violaceum. Factors such as bacterial density, frequency of administration and concentration of extract were analysed. Plant samples were collected from Thrissur District, Kerala, India. Response surface modelling of factors by Box-Behnken approach was employed for optim...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Kolaviron and selenium reduce hydrogen peroxide-induced alterations of the inflammatory response
Publication date: Available online 17 February 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Tebekeme OkokoAbstractThe abilities of kolaviron and selenium (either separately or in combination) to prevent hydrogen peroxide-induced alterations in cell viability and activation were investigated. The cell line U937 was incubated with the antioxidants (i.e. kolaviron or selenium) for 24 h before exposure to hydrogen peroxide and cell viability was assessed via trypan blue dye exclusion assay. The U937 cells were also transformed to the macrophage form, incubated with the antioxidants before exposure t...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid
Publication date: Available online 22 February 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Edy Setiti Wida Utami, Sucipto Hariyanto, Yosephine Sri Wulan ManuharaAbstractA protocol for genetic transformation mediated by Agrobacterium tumefaciens and production of transgenic Dendrobium lasianthera has been developed for the first time. The 8-week-old protocorm explants were used as target of transformation with Agrobacterium tumefaciens strain LBA4404 carrying plasmid pG35SKNAT1. Several parameters such as infection period, Agrobacterium density, concentration of acetosyringone, and co-cultivation ...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Decolorization of Textile Reactive Dyes by Bacterial Monoculture and Consortium Screened from Textile Dyeing Effluent
In this study, five dye decolorizing bacteria were isolated from dyeing effluent using selective enrichment culture in Bushnell-Haas (BH) medium amended with co-substrate (i.e. glucose, yeast extract) and 100 mg L−1 of each commercially available reactive dyes viz. Novacron Orange FN-R, Novacron Brilliant Blue FN-R, Novacron Super Black G, Bezema Yellow S8-G and Bezema Red S2-B. The isolated bacteria were identified and assigned as Neisseria sp., Vibrio sp., Bacillus sp., Bacillus sp. and Aeromonas sp. based on their phenotypic (cultural, morphological, physiological and biochemical characteristic) observation. The d...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

In silico thermodynamic stability of mammalian adaptation and virulence determinants in polymerase complex proteins of H9N2 virus
In this study; eight Egyptian LPAI-H9N2 viruses isolated from apparent healthy chickens and quails from 2014 to 2016. Characterization of complete nucleotide sequences, phylogenetic and mutation analysis were carried out. The measurement of thermodynamic stability of the H9N2 polymerase protein in comparison to human H3N2 and H1N1 proteins was carried out using in silico method. Phylogenetic analysis of these viruses revealed a close relationship to viruses isolated from neighboring Middle Eastern countries with an average of 96–99% homology. They are sharing the common ancestor A/quail/Hong Kong/G1/1997 (G1-Like) withou...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Rice straw fermentation by Schizophyllum commune ARC-11 to produce high level of xylanase for its application in pre-bleaching
Publication date: Available online 23 February 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Archana Gautam, Amit Kumar, Amit Kumar Bharti, Dharm DuttAbstractRice straw is valuable resource that has been used as substrate for cost effective production of xylanase under solid-state fermentation by a newly isolated white rot fungi, S. commune ARC-11. Out of eleven carbon sources tested, rice straw was found most effective for the induction of xylanase that produced 4288.3 IU/gds of xylanase by S. commune ARC-11. Maximum xylanase production (6721.9 IU/gds) was observed on 8th day of incubation at ...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research