Journal of Genetic Engineering and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Evaluation of genetic diversity in wild populations of Peganum harmala L., a medicinal plant
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Ranya EL-Bakatoushi, Dalia Gamil Aseel AhmedAbstractPeganum harmala L. is a perennial herbaceous plant and can be a future drug due to its wide medicinal purposes. Despite its economic importance, the molecular genetics of P. harmal have not yet been studied in detail. Genetic diversity of 12 P. harmala genotypes were investigated by using Inter-Simple Sequence Repeats (ISSR), PCR-RFLP of rDNA-ITS, PCR-SSCP of rDNA-ITS and Simple Sequence Repeat (SSR) markers. The level of polymorphism revealed by ITS-SSCP is t...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Assessment of genetic diversity in Colletotrichum falcatum Went accessions based on RAPD and ISSR markers
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Prittesh Patel, B.K. Rajkumar, Preeti Parmar, Rusabh Shah, R. KrishnamurthyAbstractSugarcane is susceptible to red rot disease caused by phytopathogenic fungus Colletotrichum falcatum Went which ultimately affect the economy of farmers as well as sugar based industry. One of the various ways to control this devastating disease is to develop disease resistance sugarcane cultivar and this requires the complete understanding of genetic makeup of pathogen. Although South Gujarat is well known sugarcane cultivating ...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Genetic variation of salt-stressed durum wheat (Triticum turgidum subsp. durum Desf.) genotypes under field conditions and gynogenetic capacity
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Olfa Ayed-Slama, Imen Bouhaouel, Zoubeir Chamekh, Youssef Trifa, Ali Sahli, Nadhira Ben Aissa, Hajer Slim-AmaraAbstractAgriculture has new challenges against the climate change: the preservation of genetic resources and the rapid creation of new varieties better adapted to abiotic stress, specially salinity. In this context, the agronomic performance of 25 durum wheat (Triticum turgidum subsp. durum Desf.) genotypes (nineteen landraces and six improved varieties), cultivated in two semi-arid regions in the cent...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Molecular insights into the genetic and haplotype diversity among four populations of Catla catla from Madhya Pradesh revealed through mtDNA cyto b gene sequences
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): R.K. Garg, Vaishali MishraAbstractIn the present investigation, the genetic structure of four populations of Catla catla, sequences of mitochondrial gene, cytochrome b (cyto b) from four populations were sequenced and analyzed. The sequences of mitochondrial regions revealed high haplotype diversity and low nucleotide diversity. The lowest 249 polymorphic sites and 0.00 parsimony informative sites were detected in populations of Fish Federation Pond (CCFFB) whereas highest 330 polymorphic sites and 56 parsimony...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Mohsen Hesami, Mohammad Hosein Daneshvar, Mohsen Yoosefzadeh-Najafabadi, Milad AlizadehAbstractFicus religiosa is known as a long-lived multipurpose forest tree. The tree plays an important role for religious, medicinal, and ornamental purposes. However, the propagation rate of Ficus religiosa is low in natural habitat so the plant tissue culture techniques are an applicable method for multiplication of this valuable medicinal plants. Thus, the aim of this study is to understand the effect of different auxin/cy...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Utilization of horticultural waste (Apple Pomace) for multiple carbohydrase production from Rhizopus delemar F2 under solid state fermentation
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Shruti Pathania, Nivedita Sharma, Shweta HandaAbstractThe brown rot fungus Rhizopus delemar F2 was shown to produce extracellular thermostable and multiple carbohydrase enzymes. The potential of Rhizopus delemar F2 in utilizing apple pomace under solid state fermentation (SSF) is the purpose of the study. Solid state fermentation (SSF) is a very effective technique opposed to submerged fermentation in various aspects. Enhanced production of multiple carbohydrases 18.20 U g−1 of cellulose, 158.30 U gâˆ...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Optimization of germination, callus induction, and cell suspension culture of African locust beans Parkia biglobosa (Jacq.) Benth
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Mohamed S. Abbas, Hattem M. El-Shabrawi, Amira Sh. Soliman, Mai A. SelimAbstractThe present study was carried out to determine the best pre-sowing treatments that can enhance the germination and seedling growth of Parkia biglobosa (Jacq.) Also, to establish and long-term maintenance of calli and cell suspension cultures . The result of various pre-sowing treatments showed that seeds soaked in concentrated H2SO4 treatment appeared the highest germination percentage, higher value of plant height, number of leaves...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

A common neighbor based technique to detect protein complexes in PPI networks
We present a technique called PROtein COmplex DEtection based on common neighborhood (PROCODE) that considers the inherent organization of protein complexes as well as the regions with heavy interactions in PPI networks to detect protein complexes. Initially, the core of the protein complexes is detected based on the neighborhood of PPI network. Then a merging strategy based on density is used to attach proteins and protein complexes to the core-protein complexes to form biologically meaningful structures. The predicted protein complexes of PROCODE was evaluated and analyzed using four PPI network datasets out of which thr...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Omics based approach for biodiscovery of microbial natural products in antibiotic resistance era
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): N. Chandra Mohana, H.C. Yashavantha Rao, D. Rakshith, P.R. Mithun, B.R. Nuthan, S. SatishAbstractThe need for a new antibiotic pipeline to confront threat imposed by resistant pathogens has become a major global concern for human health. To confront the challenge there is a need for discovery and development of new class of antibiotics. Nature which is considered treasure trove, there is re-emerged interest in exploring untapped microbial to yield novel molecules, due to their wide array of negative effects ass...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Augmented cellulase production by Bacillus subtilis strain MU S1 using different statistical experimental designs
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): C.P. Sreena, Denoj SebastianAbstractThe production of cellulase by Bacillus subtilis MU S1, a strain isolated from Eravikulam National Park, was optimized using one-factor-at-a-time (OFAT) and statistical methods. Physical parameters like incubation temperature and agitation speed were optimized using OFAT and found to be 40 °C and 150 rpm, respectively, whereas, medium was optimized by statistical tools. Plackett-Burman design (PBD) was employed to screen the significant variables that highly influence ce...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Bioprospecting of indigenous resources for the exploration of exopolysaccharide producing lactic acid bacteria
In this study, thirty-nine LAB were isolated from different food samples. The isolates were identified as genus Leuconostoc, Weissella and Streptococcus based on genotypic and phenotypic characteristics. Screening revealed that only eight isolates can produce dextransucrase in high titres. Fermentation conditions of dextran producing LAB was optimized. The results indicated that Weissella confusa exhibited maximum specific activity (1.50 DSU mg−1) in 8 h at 25 °C with pH 7.5. Dextran produced from Weissella proved to be a useful alternative to commercially used dextran produced by Leuconostoc mesenteroides in in...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Production and characterisation of exopolysaccharide from Streptomyces carpaticus isolated from marine sediments in Egypt and its effect on breast and colon cell lines
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Manal S. Selim, Shaimaa K. Amer, Sahar S. Mohamed, Marwa M. Mounier, Hala M. RifaatAbstractTwenty streptomycete strains were isolated from marine sediment samples collected from Nabq area, Sharm El-Sheikh, Red Sea Coast, Egypt. Four of them produce exopolysaccharides (EPS) showing marked in vitro antitumor activities. Morphological and cultural characteristics of the most significant strain (No. 3) were shown. Moreover, the sequence of this strain showed similarity with Streptomyces carpaticus. The results reve...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Partial purification and characterization of serine protease produced through fermentation of organic municipal solid wastes by Serratia marcescens A3 and Pseudomonas putida A2
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): Asif Iqbal, Al Hakim, Md. Saddam Hossain, Mohammad Rejaur Rahman, Kamrul Islam, Md. Faisal Azim, Jahed Ahmed, Md. Assaduzzaman, Md. Mozammel Hoq, Abul Kalam AzadAbstractProteolytic bacteria isolated from municipal solid wastes (MSW) were identified as Serratia marcescens A3 and Pseudomonas putida A2 based on 16S rDNA sequencing. Protease produced through fermentation of organic MSW by these bacteria under some optimized physicochemical parameters was partially purified and characterized. The estimated molecular...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars
Publication date: June 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 1Author(s): F.A. Hellal, H.M. El-Shabrawi, M. Abd El-Hady, I.A. Khatab, S.A.A. El-Sayed, Chedly AbdellyAbstractIn order to investigate the effects of drought stress on germination components of barley cultivars, a laboratory experiment was conducted in a factorial randomized complete design with four replications. The controlled experiment included ten of Egyptian barley cultivars namely; (Giza 123, 124, 125, 126, 127, 129, 130, 134, 135 and 2000) as first factor. The second factor included 4 levels of drought stress induc...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Impact of Apo E gene polymorphism on HCV therapy related outcome in a cohort of HCV Egyptian patients
Conclusion: This study supports the hypothesis of the protective impact of Apo E4 allele that favors viral clearance of HCV infection and its recovery after combined therapy, while the Apo E3 allele is considered as a particular risk factor for the chronicity in HCV patients and resistance to therapy. Whereas the Apo E2 allele confers a resistance to HCV infection at a time of exposure. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research