Screening of plant growth promoting traits in heavy metals resistant bacteria: Prospects in phytoremediation
In this study, we isolated twenty seven (NF1-NF27) chromium resistant bacteria. The bacteria were tested for heavy metals (Cr, Zn, Cu, Ni, Pb and Co) resistance, Cr(VI) reduction and PGPB characters (phosphate solubilization, production of IAA and siderophores). The results showed that the bacterial isolates resist to heavy metals and reduce Cr(VI), with varying capabilities. 37.14% of the isolates have the capacity of solubilizing phosphate, 28.57% are able to produce siderophores and all isolates have the ability to produce IAA. Isolate NF2 that showed high heavy metal resistance and plant growth promotion characteristic...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Evaluation of the alleviative role of Chlorella vulgaris and Spirulina platensis extract against ovarian dysfunctions induced by monosodium glutamate in mice
In conclusion, Chlorella vulgaris and Spirulina platensis showed potential alleviative role against the monosodium glutamate ovarian dysfunction. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Synthesis of silver nanoparticles by Bacillus clausii and computational profiling of nitrate reductase enzyme involved in production
Publication date: Available online 28 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Koel Mukherjee, Rashmi Gupta, Gourav Kumar, Sarita Kumari, Saptaswa Biswas, Padmini PadmanabhanAbstractBiogenic synthesis of silver nanoparticles using microorganisms has found interest recently since last decade because of their prospect to synthesize nanoparticles of various size, shape and morphology which are eco-friendly. Here, an eco-friendly method for production of silver nanoparticles from Bacillus clausii cultured from Enterogermina is explored. Along with the biosynthesis and conformity test, in sili...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Phylogenetic diversity and biotechnological potentials of marine bacteria from continental slope of eastern Arabian Sea
Publication date: Available online 29 June 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Arakkaveettil Kabeer Farha, Thasneem TR, Aswathy Purushothaman, Jaseetha Abdul Salam, Abdulla Mohamed HathaAbstractMarine environments are substantially untapped source for the isolation of bacteria with the capacity to produce various extracellular hydrolytic enzymes, which have important ecological roles and promising biotechnological applications. Hydrolases constitute a class of enzymes widely distributed in nature from bacteria to higher eukaryotes. Marine microbial communities are highly diverse and have ...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Expression, purification and biological characterisation of recombinant human irisin (12.5 kDa)
Publication date: Available online 2 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Kalpana Panati, Venkata Ramireddy Narala, Vydyanath R. Narasimha, Madhavi Derangula, Venkat R.R. Arva Tatireddigari, Suneetha YeguvapalliAbstractFibronectin type III domain containing 5 (FNDC5) is a transmembrane protein. Upon cleavage, it yields a peptide called irisin that is supposedly bind to an unknown receptor and facilitates browning of white adipose tissue (WAT). Increased levels of irisin are associated with increased levels of energy expenditure markers PGC-1α, UCP-1, besides abundance of beige adipoc...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India
Publication date: Available online 3 July 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): S. Muthukrishnan, T. Senthil Kumar, A. Gangaprasad, F. Maggi, M.V. RaoAbstractCeropegia thwaitesii Hook (Asclepiadaceae), an endemic plant species, due to habitat destruction and over exploitation has a very restricted distribution in the Western Ghats of Tamil Nadu, India. The present wrok aimed to determine the chemical composition, the total phenolic (TPC), flavonoid (TFC) and tannin content (TEC), and to assess the antioxidant properties of various extracts of in vivo plants (IVP) and in vitro regenerated pl...
Source: Journal of Genetic Engineering and Biotechnology - July 5, 2018 Category: Biotechnology Source Type: research

Expression, purification and biological characterisation of recombinant human irisin (12.5  kDa)
Publication date: Available online 2 July 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Kalpana Panati, Venkata Ramireddy Narala, Vydyanath R. Narasimha, Madhavi Derangula, Venkat R.R. Arva Tatireddigari, Suneetha Yeguvapalli Fibronectin type III domain containing 5 (FNDC5) is a transmembrane protein. Upon cleavage, it yields a peptide called irisin that is supposedly bind to an unknown receptor and facilitates browning of white adipose tissue (WAT). Increased levels of irisin are associated with increased levels of energy expenditure markers PGC-1α, UCP-1, besides abundance of beige adip...
Source: Journal of Genetic Engineering and Biotechnology - July 4, 2018 Category: Biotechnology Source Type: research

Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India
Publication date: Available online 3 July 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): S. Muthukrishnan, T. Senthil Kumar, A. Gangaprasad, F. Maggi, M.V. Rao Ceropegia thwaitesii Hook (Asclepiadaceae), an endemic plant species, due to habitat destruction and over exploitation has a very restricted distribution in the Western Ghats of Tamil Nadu, India. The present wrok aimed to determine the chemical composition, the total phenolic (TPC), flavonoid (TFC) and tannin content (TEC), and to assess the antioxidant properties of various extracts of in vivo plants (IVP) and in vitro regenerated p...
Source: Journal of Genetic Engineering and Biotechnology - July 4, 2018 Category: Biotechnology Source Type: research

Phylogenetic diversity and biotechnological potentials of marine bacteria from continental slope of eastern Arabian Sea
Publication date: Available online 29 June 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Arakkaveettil Kabeer Farha, Thasneem TR, Aswathy Purushothaman, Jaseetha Abdul Salam, Abdulla Mohamed Hatha Marine environments are substantially untapped source for the isolation of bacteria with the capacity to produce various extracellular hydrolytic enzymes, which have important ecological roles and promising biotechnological applications. Hydrolases constitute a class of enzymes widely distributed in nature from bacteria to higher eukaryotes. Marine microbial communities are highly diverse and have...
Source: Journal of Genetic Engineering and Biotechnology - June 30, 2018 Category: Biotechnology Source Type: research

Improvement of cellulose degradation by cloning of endo- β-1, 3-1, 4 glucanase (bgls) gene from Bacillus subtilis BTN7A strain
Publication date: Available online 28 June 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Wafaa K. Hegazy, Mohamed S. Abdel-Salam, Azhar A. Hussain, Hoda H. Abo-Ghalia, Safa S. Hafez The aim of this study is to construct a new recombinant strain able to degrade cellulose efficiently. The endo-β-1, 3-1, 4 glucanase (bgls) gene was cloned from Bacillus subtilis BTN7A strain by using PCR technique. The specific primers of bgls gene were deduced. Optimization of PCR mixture and program were identified. The nucleotide sequence of bgls was placed in the public domain (GenBank accession number KM0...
Source: Journal of Genetic Engineering and Biotechnology - June 29, 2018 Category: Biotechnology Source Type: research

Evaluation of the alleviative role of Chlorella vulgaris and Spirulina platensis extract against ovarian dysfunctions induced by monosodium glutamate in mice
In conclusion, Chlorella vulgaris and Spirulina platensis showed potential alleviative role against the monosodium glutamate ovarian dysfunction. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - June 29, 2018 Category: Biotechnology Source Type: research

Screening of plant growth promoting traits in heavy metals resistant bacteria: Prospects in phytoremediation
In this study, we isolated twenty seven (NF1-NF27) chromium resistant bacteria. The bacteria were tested for heavy metals (Cr, Zn, Cu, Ni, Pb and Co) resistance, Cr(VI) reduction and PGPB characters (phosphate solubilization, production of IAA and siderophores). The results showed that the bacterial isolates resist to heavy metals and reduce Cr(VI), with varying capabilities. 37.14% of the isolates have the capacity of solubilizing phosphate, 28.57% are able to produce siderophores and all isolates have the ability to produce IAA. Isolate NF2 that showed high heavy metal resistance and plant growth promotion characteristic...
Source: Journal of Genetic Engineering and Biotechnology - June 29, 2018 Category: Biotechnology Source Type: research

Synthesis of silver nanoparticles by Bacillus clausii and computational profiling of nitrate reductase enzyme involved in production
Publication date: Available online 28 June 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Koel Mukherjee, Rashmi Gupta, Gourav Kumar, Sarita Kumari, Saptaswa Biswas, Padmini Padmanabhan Biogenic synthesis of silver nanoparticles using microorganisms has found interest recently since last decade because of their prospect to synthesize nanoparticles of various size, shape and morphology which are eco-friendly. Here, an eco-friendly method for production of silver nanoparticles from Bacillus clausii cultured from Enterogermina is explored. Along with the biosynthesis and conformity test, in si...
Source: Journal of Genetic Engineering and Biotechnology - June 29, 2018 Category: Biotechnology Source Type: research

Antibacterial activity of soil bacteria isolated from Kochi, India and their molecular identification
Publication date: Available online 14 June 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Davis Gislin, Dorairaj Sudarsanam, Gnanaprakasam Antony Raj, Kathirvelu Baskar The present study, deal about the antibiosis activity of soil bacteria, isolated from 10 different locations of rhizosphere and diverse cultivation at Kochi, Kerala, India. The bacteria were isolated by standard serial dilution plate techniques. Morphological characterization of the isolate was done by Gram’s staining and found that all of them gram positive. Isolated bacteria were tested against 6 human pathogens viz., Esch...
Source: Journal of Genetic Engineering and Biotechnology - June 14, 2018 Category: Biotechnology Source Type: research

In silico analysis of squalene synthase in Fabaceae family using bioinformatics tools
Publication date: Available online 6 June 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Zahra Aminfar, Masoud Tohidfar Triterpenoid saponins are a diverse group of bioactive compounds, which are used for possessing of many biomedical and pharmaceutical products. Generally, squalene synthase (SQS) is defined as an emerging and essential branch point enzyme far from the major pathway of isoprenoids biosynthetic and a latent adjusting point, which manages carbon flux into triterpenes biosynthesis and sterols. The present study deals with the detailed characterization of SQS by bioinformatics appr...
Source: Journal of Genetic Engineering and Biotechnology - June 6, 2018 Category: Biotechnology Source Type: research