Journal of Genetic Engineering and Biotechnology 
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Healthcare-associated (HA) and community-associated (CA) methicillin resistant Staphylococcus aureus (MRSA) in Bangladesh – Source, diagnosis and treatment
This study reported source, diagnosis and treatment of HA-MRSA and CA-MRSA. A total of sixty-five clinical samples (urine, pus, wound swab) were collected from clinical origin of Dhaka city, Bangladesh. All the isolates were tested phenotypically by conventional methods and genotypically by PCR targeting nuc, pvl and mecA genes. Finally sequencing was carried out for pvl gene to know the mutagenic variation or any amino acid changes in pvl gene. Chi square test was employed for statistical analysis. Patients of age group 51–60 years are more susceptible (46.15%) to MRSA, CA-MRSA or HA-MRSA infection. Female are (32.30%...
Source: Journal of Genetic Engineering and Biotechnology - June 5, 2018 Category: Biotechnology Source Type: research
In silico studies on bacterial xylanase enzyme: Structural and functional insight
Publication date: Available online 31 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Bhramar Dutta, Aparna Banerjee, Priyanka Chakraborty, Rajib Bandopadhyay Xylans are the second most abundant form of hemicelluloses and are the second most abundant polysaccharide in nature after cellulose. To degrade xylan, microbes produce mainly xylanase enzyme. Wide range of microorganisms like fungi, bacteria, yeast, marine algae etc. are capable of producing xylanase. Main source of xylanase is fungi but industrial production of bacterial xylanase is low cost, easy downstream process and high produc...
Source: Journal of Genetic Engineering and Biotechnology - May 31, 2018 Category: Biotechnology Source Type: research
Increased level of B cell differentiation factor in systemic lupus erythematosus patients
Conclusion BCDF and IgM are increased in SLE patients and are promissing diagnostic markers for SLE. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - May 30, 2018 Category: Biotechnology Source Type: research
Biodegradation of feather waste by keratinase produced from newly isolated Bacillus licheniformis ALW1
Publication date: Available online 28 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Azza M. Abdel-Fattah, Mamdouh S. El-Gamal, Siham A. Ismail, Mohamed A. Emran, Amal M. Hashem Keratinase are proteolytic enzymes which have gained much attention to convert keratinous wastes that cause huge environmental pollution problems. Ten microbial isolates were screened for their keratinase production. The most potent isolate produce 25.2 U/ml under static condition and was primarily identified by partial 16s rRNA gene sequence as Bacillus licheniformis ALW1. Optimization studies for the fermenta...
Source: Journal of Genetic Engineering and Biotechnology - May 29, 2018 Category: Biotechnology Source Type: research
Purification and characterization of alkaline protease with novel properties from Bacillus cereus strain S8
Publication date: Available online 26 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): B.K.M Lakshmi, D. Muni Kumar, K.P.J Hemalatha Proteases are the hydrolytic enzymes which hydrolyzes peptide bond between proteins with paramount applications in pharmaceutical and industrial sector. Therefore production of proteases with efficient characteristics of biotechnological interest from novel strain is significant. Hence, in this study, an alkaline serine protease produced by Bacillus cereus strain S8 (MTCC NO 11901) was purified and characterized. The alkaline protease was purified by ammonium s...
Source: Journal of Genetic Engineering and Biotechnology - May 26, 2018 Category: Biotechnology Source Type: research
Enhancement of nematicidal potential through cloning and expression of chitinase gene from Bacillus subtilis subsp. Subtilis BTN7A strain
Publication date: Available online 23 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Mohamed S. Abdel-Salam, Hoda H. Ameen, Abdallah S.M. Kassab, Ahmed E.A. Mahgoob, Usama S. Elkelany A gene encoding chitinase from B. subtilis has been isolated after optimization of PCR conditions. It was cloned with two different prometers, T7 promoter of the pJET1.2/blunt cloning vector and the SP6 promoter of pGEM®-T Easy vector. After transforming E. coli DH5α, two transformants were selected, CHI-NRC-4 from the first vector and T-CHI-NRC-6 from the second vector, and used for further studies. The ...
Source: Journal of Genetic Engineering and Biotechnology - May 24, 2018 Category: Biotechnology Source Type: research
Detection of myostatin gene MSTN in some goat breeds (Capra hircus)
Publication date: Available online 26 April 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Y.A. Dowidar, M.A. El-Sayed, Aly M. Elrefy, Hytham E. Shoura Till now not information about myostatin MSTN gene in Egyptian goat breeds. Here we show more information about MSTN in some Egyptian goat breeds to enrich the database with new sequences for Egyptian goat breeds. Our conducted study focused on detection and identifying the MSTN gene as a candidate gene of the muscles growth trait in three goat breeds (Zaraibi, Baladi and Damascus). We found the similarity between the registered sequences with...
Source: Journal of Genetic Engineering and Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research
Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent
Publication date: Available online 27 April 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Kiptoo Geoffry, Rajeshwara N. Achur Among different sources of lipases, fungal lipases have continued to attract a wide range of applications. Further, halophilic lipases are highly desirable for biodiesel production due to the need to mitigate environmental pollution caused as result of extensive use of fossil fuels. However, currently, the high production cost limits the industrial application of lipases. In order to address this issue, we have attempted to optimize lipase production by Fusarium solani ...
Source: Journal of Genetic Engineering and Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research
Assessment of genetic diversity in Salvadora persica L. based on inter simple sequence repeat (ISSR) genetic marker
In this study, ISSR markers were used to establish the level of genetic relationships and polymorphism 50 genotypes of Salvadora persica collected from 6 different regions of Hormozgan province. The ISSR analysis with 9 anchored primers also generated 105 scorable loci, of which 85 were polymorphic (80.95%). Parameters of genetic diversity and its partitioning were calculated. The genetic analysis demonstrated that S. persica maintain relatively high genetic diversity (PIC was 0.63, Na was 1.27 and Ho and He were 0.15 and 0.17 respectively). The coefficient of genetic differentiation among populations based on FST equaled ...
Source: Journal of Genetic Engineering and Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research
Isolation and characterization of Bacillus sp. strain BC01 from soil displaying potent antagonistic activity against plant and fish pathogenic fungi and bacteria
Publication date: Available online 7 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Md Javed Foysal, Asura Khanam Lisa Fungal and bacterial pathogens infect a diverse range of hosts including various plant and animal species. Fungal and bacterial diseases, especially of plants and aquatic animals, such as fish, lead to significant damage to crops and aquaculture, respectively, worldwide. The present study was conducted to isolate and characterize potent Bacillus strains with significant antagonistic activity against the major plant and fish pathogenic fungi and bacteria. We randomly collect...
Source: Journal of Genetic Engineering and Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research
Study on the potential of cold-active lipases from psychrotrophic fungi for detergent formulation
Publication date: Available online 7 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Sanjay Sahay, Deepak Chouhan Lipases from psychrotrophic fungal isolates BPF4 and BPF6 identified as Penicilium canesense and Pseudogymnoascus roseus respectively were characterized for their compatibility towards laundry detergent. BPF4 and BPF6 lipases showed maximum activity at pH 11 and 9 respectively and at 40 °C. The residual activities at 20 °C and 4 °C of BPF4 lipase were 35% and 20% and of BPF6 lipase were 70% and 20 °C respectively. Both the enzymes were stable at 4 °C, 20 °C and ...
Source: Journal of Genetic Engineering and Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research
Molecular diversity of internal transcribed spacer among the monoconidial isolates of Magnaporthe oryzae isolated from rice in Southern Karnataka, India
Publication date: Available online 19 May 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): D. Jagadeesh, M.K. Prasanna Kumar, R. Chandrakanth, N.S. Devaki Blast disease of rice plant is caused by Magnaporthe oryzae (anamorph Pyricularia oryzae). This disease is recognized to be one of the most serious diseases of rice crop around the world. A total of 72 monoconidial isolates of M. oryzae obtained from blast disease samples collected around Southern Karnataka were characterized using internal transcribed spacers of the ribosomal DNA sequences. These were analyzed by comparing with already depos...
Source: Journal of Genetic Engineering and Biotechnology - May 22, 2018 Category: Biotechnology Source Type: research
Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study
Publication date: Available online 13 April 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Ahyar Ahmad, Rosana Agus, Muh. Nasrum Massi, Rosdiana Natzir, Radha Madhyastha, Harish Kumar Madhyastha, Masugi Maruyama The appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development will stop the epidemic of TB. Some M. tuberculosis antigens, one of which is MPT83,...
Source: Journal of Genetic Engineering and Biotechnology - April 14, 2018 Category: Biotechnology Source Type: research
High level expression and purification of recombinant flounder growth hormone in E. coli
Publication date: Available online 9 April 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Tae-Jin Choi, Temesgen Tola Geletu Recombinant flounder growth hormone was overproduced in E. coli by using codon optimized synthetic gene and optimized expression conditions for high level production. The gene was cloned into PET-28a expression vector and transformed into E. coli BL21 (DE3). Induction at lower temperature, lower IPTG concentrations and richer growth media during expression resulted in increased expression level. The protein expression profile was analyzed by SDS-PAGE, the authenticity was...
Source: Journal of Genetic Engineering and Biotechnology - April 10, 2018 Category: Biotechnology Source Type: research
Micropropagation protocol for Antigonon leptopus an important ornamental and medicinal plant
Publication date: Available online 5 April 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Zenna Fawzia Ghareeb, Lobna S. Taha The effect of some factors on in vitro consecutive micropropagation behavior of Antigonon leptopus was examined including those of culture establishment, shootlets multiplication, rooting and acclimatization stages. The highest percent of aseptic cultures and survival of explants (100%) were obtained as a result of using Clorox 10% for 3 min followed by MC 0.1% for 2 min while, using each of them individually (Clorox 20% or MC 0.1%) for 5 min caused the highest per...
Source: Journal of Genetic Engineering and Biotechnology - April 6, 2018 Category: Biotechnology Source Type: research
