Journal of Genetic Engineering and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Immobilization of thermostable exo-inulinase from mutant thermophilic Aspergillus tamarii-U4 using kaolin clay and its application in inulin hydrolysis
In this study, attempts were made to immobilize purified exo-inulinase from mutant thermophic Aspergillus tamarii-U4 onto Kaolinite clay by covalent bonding cross-linked with glutaraldehyde with an immobilization yield of 66% achieved. The free and immobilized inulinases were then characterized and characterization of the enzymes revealed that temperature and pH optima for the activity of the free and immobilized enzymes were both 65 °C and pH 4.5 respectively. The free inulinase completely lost its activity after incubation at 65 °C for 6 h while the immobilized inulinase retained 16.4% of its activity under the sam...
Source: Journal of Genetic Engineering and Biotechnology - April 5, 2018 Category: Biotechnology Source Type: research
Screening of potential probiotic lactic acid bacteria and production of amylase and its partial purification
Publication date: Available online 27 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Tallapragada Padmavathi, Rayavarapu Bhargavi, Purushothama Rao Priyanka, Naige Ranganath Niranjan, Pogakul Veerabhadrappa Pavitra Probiotics are the healthy living bacteria when administered in adequate amounts confers health benefits in the host. The main objective of present study was to screen the bacteria for potential probiotic characters and enzyme production. The probiotic characters like tolerance to low pH, bile salts, antibiotic sensitivity, hydrophobicity and auto-aggregation properties were...
Source: Journal of Genetic Engineering and Biotechnology - March 28, 2018 Category: Biotechnology Source Type: research
Screening of anti-inflammatory phytocompounds from Crateva adansonii leaf extracts and its validation by in silico modeling
Publication date: Available online 23 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Rathinavel Thirumalaisamy, Subramanian Ammashi, Govarthanan Muthusamy Anti-inflammatory phytocompounds from Crateva adansonii DC leaf extracts were identified by GCMS analysis and its anti-inflammatory potential was evaluated by in silico molecular docking study against inflammatory molecular targets. Three different (Aqueous, Methanol and Petroleum ether) dried leaf extracts of Crateva adansonii were obtained from soxhlet extraction method. Preliminary phytoconstituents analysis of three different leaf ...
Source: Journal of Genetic Engineering and Biotechnology - March 24, 2018 Category: Biotechnology Source Type: research
Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors
Publication date: Available online 21 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Ammar Mohammed Ahmed Ali, Mawahib ElAmin Mohamed El-Nour, Sakina Mohamed Yagi The present study was aimed at determining total phenolic and flavonoid contents and studying the antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome and callus, 6-gingerol and 6-shogaol and callus treated with elicitors. Petroleum ether (PE) and chloroform: methanol (1:1, v/v) (CM) extracts were prepared by maceration. Highest total phenolic content was obtained from the CM extract (60.34 ± 0.43 mg gallic...
Source: Journal of Genetic Engineering and Biotechnology - March 22, 2018 Category: Biotechnology Source Type: research
In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects
Publication date: Available online 19 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Saikat Gantait, Jitendriya Panigrahi Adhatoda vasica Nees, belonging to family Acanthaceae, is a well-known medicinal plant. It is endorsed for its pyrroloquinazoline alkaloids and its derivatives, such as vasicine and vasicinone. Germinating A. vasica seeds is a tedious task; on that account, vegetative propagation is the preferred method for its multiplication. For rapid and large-scale multiplication, germplasm conservation as well as secondary metabolites production, in vitro culture of A. vasica was ...
Source: Journal of Genetic Engineering and Biotechnology - March 20, 2018 Category: Biotechnology Source Type: research
Assessment of Ki-67 as a potential biomarker in patients with breast cancer
In conclusion; our study supports the finding that tissue Ki-67 expression may add prognostic information to that obtained from classical prognostic factors and can provide data of significant value to other important prognostic indicators such as pathological grading, and axillary lymph node involvement. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - March 11, 2018 Category: Biotechnology Source Type: research
Partial purification and characterization of exoinulinase produced from Bacillus sp.
In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was selected with 25.10 U/mL for further studies. The best inulinase producing colony was identified by partial 16S rRNA gene sequence as Bacillus sp. The crude inulinase was purified by using ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE – sephacel and obtained 1.9 purification fold with total activity 293 U. The purified enzyme was subjected to characterization studies and it was f...
Source: Journal of Genetic Engineering and Biotechnology - March 9, 2018 Category: Biotechnology Source Type: research
Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants
Publication date: Available online 6 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Rania Samy Hanafy, Samia Ageeb Akladious This experiment assessed the biochemical changes in fenugreek plants exposed to gamma radiation. Two pot experiments were carried out during two growing seasons of 2015 and 2016. Seeds were subjected to five doses of gamma irradiation (25, 50, 100, 200 and 400 Gy) and were immediately planted into soil pots in a greenhouse. The experimental analysis was performed in M1 and M2 generations. Significant differences between irradiated and control plants were detected ...
Source: Journal of Genetic Engineering and Biotechnology - March 7, 2018 Category: Biotechnology Source Type: research
Elevated carotenoids in staple crops: The biosynthesis, challenges and measures for target delivery
Publication date: Available online 6 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Adebanjo Ayobamidele Badejo Poverty eradication and global food security are among the targets of world leaders, most especially combating the scourge of hidden hunger. Provitamin A carotenoids cannot be synthesized de novo by human and so it must be taken as part of the diet. The deficiency of which is causing almost 6000 sights to be lost daily in most developing countries because of the monotonous starchy diets lacking substantial amount of carotenoid. Conventional breeding as well as genetic engineering...
Source: Journal of Genetic Engineering and Biotechnology - March 7, 2018 Category: Biotechnology Source Type: research
Effect of vitamins and cell constructions on the activity of microbial fuel cell battery
Publication date: Available online 3 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Dena Z. Khater, K.M. El-Khatib, Rabeay Y.A. Hassan Construction of efficient performance of microbial fuel cells (MFCs) requires certain practical considerations. In the single chamber microbial fuel cell, there is no border between the anode and the cathode, thus the diffusion of the dissolved oxygen has a contrary effect on the anodic respiration and this leads to the inhibition of the direct electron transfer from the biofilm to the anodic surface. Here, a fed-batch single chambered microbial fuel cell...
Source: Journal of Genetic Engineering and Biotechnology - March 4, 2018 Category: Biotechnology Source Type: research
Omics based approach for biodiscovery of microbial natural products in antibiotic resistance era
Publication date: Available online 2 March 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): N. Chandra Mohana, H.C. Yashavantha Rao, D. Rakshith, P.R. Mithun, B.R. Nuthan, S. Satish The need for a new antibiotic pipeline to confront threat imposed by resistant pathogens has become a major global concern for human health. To confront the challenge there is a need for discovery and development of new class of antibiotics. Nature which is considered treasure trove, there is re-emerged interest in exploring untapped microbial to yield novel molecules, due to their wide array of negative effects a...
Source: Journal of Genetic Engineering and Biotechnology - March 3, 2018 Category: Biotechnology Source Type: research
Five BoLA-DRB3 genotypes detected in Egyptian buffalo infected with Foot and Mouth disease virus serotype O
In conclusion, our findings suggested that the detection of different BoLA-DRB3 genotypes may be has a promising role for raising the resistance of Egyptian buffalo against FMDV especially serotype O which is prevalent in Egypt with preferring genotype AA. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - March 1, 2018 Category: Biotechnology Source Type: research
In silico thermodynamic stability of mammalian adaptation and virulence determinants in polymerase complex proteins of H9N2 virus
In this study; eight Egyptian LPAI-H9N2 viruses isolated from apparent healthy chickens and quails from 2014 to 2016. Characterization of complete nucleotide sequences, phylogenetic and mutation analysis were carried out. The measurement of thermodynamic stability of the H9N2 polymerase protein in comparison to human H3N2 and H1N1 proteins was carried out using in silico method. Phylogenetic analysis of these viruses revealed a close relationship to viruses isolated from neighboring Middle Eastern countries with an average of 96–99% homology. They are sharing the common ancestor A/quail/Hong Kong/G1/1997 (G1-Like) withou...
Source: Journal of Genetic Engineering and Biotechnology - February 24, 2018 Category: Biotechnology Source Type: research
Rice straw fermentation by Schizophyllum commune ARC-11 to produce high level of xylanase for its application in pre-bleaching
Publication date: Available online 23 February 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): Archana Gautam, Amit Kumar, Amit Kumar Bharti, Dharm Dutt Rice straw is valuable resource that has been used as substrate for cost effective production of xylanase under solid-state fermentation by a newly isolated white rot fungi, S. commune ARC-11. Out of eleven carbon sources tested, rice straw was found most effective for the induction of xylanase that produced 4288.3 IU/gds of xylanase by S. commune ARC-11. Maximum xylanase production (6721.9 IU/gds) was observed on 8th day of incubation at ...
Source: Journal of Genetic Engineering and Biotechnology - February 24, 2018 Category: Biotechnology Source Type: research
In vitro culture, transformation and genetic fidelity of Milk Thistle
Publication date: Available online 23 February 2018 Source:Journal of Genetic Engineering and Biotechnology Author(s): M.R. Rady, M.M. Saker, M.A. Matter This review article presents a consolidated explanation and provides a comprehensive description of various studies, carried out on in vitro culture and hairy root cultures of S. marianum which can be consider an alternative source of flavonolignans. To overcome the constrains of conventional propagation of silybum plant, tissue culture and advanced biotechnology proved to be an influential tool that can complement conventional breeding and accelerate silybum develop...
Source: Journal of Genetic Engineering and Biotechnology - February 24, 2018 Category: Biotechnology Source Type: research
