Transformation of Candida guilliermondii wild‐type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin‐resistant marker
Abstract
We designed an efficient transformation system for C. guilliermondii wild‐type strains. We demonstrated that the Staphylococcus aureus MRSA 252 ble coding sequence placed under the control of the yeast phosphoglycerate kinase gene transcription‐regulating regions confers phleomycin resistance to transformed C. guilliermondii cells. To illustrate the potential of this drug‐resistant cassette, we carried out the disruption of the C. guilliermondii ADE2 gene. This new dominant selectable marker represents a powerful tool to study the function of various genes in this yeast of clinical and biotechnological inter...
Source: FEMS Yeast Research - February 1, 2013 Category: Research Authors: Emilien Foureau, Vincent Courdavault, Andrew J. Simkin, Andriy A. Sibirny, Joël Crèche, Nathalie Giglioli‐Guivarc'h, Marc Clastre, Nicolas Papon Tags: Short Communication Source Type: research
The contribution of the non‐homologous region of Prs1 to the maintenance of cell wall integrity and cell viability
Abstract
The gene products of the five‐membered PRS gene family in S. cerevisiae have been shown to exist as three minimal functional entities, Prs1/Prs3, Prs2/Prs5 and Prs4/Prs5, each capable of supporting cell viability. The Prs1/Prs3 heterodimer can be regarded as the most important since its loss causes temperature sensitivity. It has been shown that the GFP signal generated by an integrated GFP‐Prs1 construct is lost in the absence of Prs3. In addition to interacting with Prs3, Prs1 also interacts with Slt2, the MAPK of the cell wall integrity (CWI) pathway. Lack of the non‐homologous region (NHR1‐1) located c...
Source: FEMS Yeast Research - January 31, 2013 Category: Research Authors: Eziuche A. Ugbogu, Sonja Wippler, Matthew Euston, Evelyn N. Kouwenhoven, Arjan PM Brouwer, Lilian M Schweizer, Michael Schweizer Tags: Research Paper Source Type: research
Molecular Characterization of Saccharomyces cerevisiae α‐pheromone by Mass Spectrometry based Peptidomics
Abstract
Using modern peptide analytical MS technology (‘Peptidomics’), it is possible to analyze yeast α‐pheromone both qualitatively and semi‐quantitatively directly from conditioned cell culture media. MS/MS analysis shows both forms of α‐pheromone (MFα and MFα’) detectable and identifiable straight from WT supernatants. In addition to the mature intact α‐pheromones, also post‐translationally modified α‐pheromone peptides and fragments thereof are found to be present in the culture medium. This molecular analytical technique is complementary to the recently described quantitation method by Rogers...
Source: FEMS Yeast Research - January 31, 2013 Category: Research Authors: Eda Bener Aksam, Martijn W.H. Pinkse, Peter D.E.M. Verhaert Tags: Short Communication Source Type: research
Transcriptional profiling of Brazilian Saccharomyces cerevisiae strains selected for semi‐continuous fermentation of sugarcane must
This study presents a genome wide analysis of the CAT‐1 and PE‐2 transcriptomes during a small‐scale fermentation process that mimicked the industrial conditions. The common and unique transcriptional responses of the two strains to the Brazilian fermentation process were identified. Environmental stress response genes were up regulated post fermenter feeding, demonstrating the impact of the prior acid wash and high glucose environment. Cell wall and oxidative stress tolerance were subsequently demonstrated to be enhanced for the industrial strains. Conversely, numerous genes involved in protein synthesis were down r...
Source: FEMS Yeast Research - January 30, 2013 Category: Research Authors: Neil A. Brown, Patrícia Alves Castro, Bárbara Castro Pimentel Figueiredo, Marcela Savoldi, Marcos Silveira Buckeridge, Mário Lúcio Lopes, Silene Cristina Lima Paullilo, Eduardo P. Borges, Henrique V. Amorim, Maria Helena S. Goldman, Diego Bonatto, Ira Tags: Research Paper Source Type: research
From the Chief Editor
(Source: FEMS Yeast Research)
Source: FEMS Yeast Research - January 14, 2013 Category: Research Authors: Jens Nielsen Tags: Editorial Source Type: research
Unravelling genomic diversity of Zygosaccharomyces rouxii complex with a link to its life cycle
Abstract
Zygosaccharomyces rouxii and the related species Zygosaccharomyces sapae (hereafter referred to as Z. rouxii complex) are protoploid hemiascomycete yeasts relevant in the elaboration and spoilage of foodstuff. Divergence of Z. rouxii complex before whole genome duplication, leading to the genus Saccharomyces, makes these yeasts very attractive for genome evolution study. Relatively little is known, however, about the diversity in this branch at the genetic and physiological levels. In this work, we investigated Z. rouxii complex, encompassing strains that in other works have been studied separately and comparin...
Source: FEMS Yeast Research - January 14, 2013 Category: Research Authors: Lisa Solieri, Tikam Chand Dakal, Maria Antonietta Croce, Paolo Giudici Tags: Research Article Source Type: research
Yeast response to LA virus indicates coadapted global gene expression during mycoviral infection
Abstract
Viruses that infect fungi have a ubiquitous distribution and play an important role in structuring fungal communities. Most of these viruses have an unusual life history in that they are propagated exclusively via asexual reproduction or fission of fungal cells. This asexual mode of transmission intimately ties viral reproductive success to that of its fungal host and should select for viruses that have minimal deleterious impact on the fitness of their hosts. Accordingly, viral infections of fungi frequently do not measurably impact fungal growth, and in some instances, increase the fitness of the fungal host. He...
Source: FEMS Yeast Research - January 2, 2013 Category: Research Authors: Robert C. McBride, Nathalie Boucher, Derek S. Park, Paul E. Turner, Jeffrey P. Townsend Tags: Research Article Source Type: research
End‐of‐life cell cycle arrest contributes to stochasticity of yeast replicative aging
Abstract
There is growing evidence that stochastic events play an important role in determining individual longevity. Studies in model organisms have demonstrated that genetically identical populations maintained under apparently equivalent environmental conditions display individual variation in lifespan that can be modeled by the Gompertz‐Makeham law of mortality. Here we report that within genetically identical haploid and diploid wild type populations, shorter‐lived cells tend to arrest in a budded state, while cells that arrest in an unbudded state are significantly longer‐lived. This relationship is particularl...
Source: FEMS Yeast Research - January 1, 2013 Category: Research Authors: Joe R. Delaney, Annie Chou, Brady Olsen, Daniel Carr, Christopher Murakami, Umema Ahmed, Sylvia Sim, Elroy H. An, Anthony S. Castanza, Marissa Fletcher, Sean Higgins, Mollie Holmberg, Jessica Hui, Monika Jelic, Ki‐Soo Jeong, Jin R. Kim, Shannon Klum, Er Tags: Research Paper Source Type: research
The zinc finger protein Gsf1 regulates Gsf2‐dependent flocculation in fission yeast
Abstract
Fission yeast flocculates non‐sexually by induction of the flocculin encoded by gsf2+ which is controlled by the positive regulator Mbx2. Here we report a novel gene designated gsf1+ found to be a negative regulator of non‐sexual flocculation. We identified gsf1+ as a multicopy suppressor of a sam2 mutation, which caused growth sensitivity to Ca2+ and also found a nonsense mutation in gsf1+ in a previously isolated gsf1 mutant. The gsf1+ gene encodes a 547 aa protein containing a Zn(2)‐Cys(6) binuclear cluster type Zinc finger motif. The Gsf1 protein localized in the nucleus, consistent with a role as a tran...
Source: FEMS Yeast Research - January 1, 2013 Category: Research Authors: Tomohiko Matsuzawa, Youko Kageyama, Kazuyoshi Ooishi, Makoto Kawamukai, Kaoru Takegawa Tags: Research Paper Source Type: research
