A system for multilocus chromosomal integration and transformation‐free selection marker rescue
Abstract
Yeast integrating plasmids (YIPs) are a versatile tool for stable integration in Saccharomyces cerevisiae. However, current YIP systems necessitate time‐ and labor‐intensive methods for cloning and selection marker rescue. Here, we describe the design, construction, and validation of a new YIP system capable of accelerating the stable integration of multiple expression constructs into different loci in the yeast S. cerevisiae. These ‘directed pop‐out’ plasmids enable a simple, two‐step integration protocol that results in a scarless integration alongside a complete rescue of the selection marker. Thes...
Source: FEMS Yeast Research - October 10, 2014 Category: Research Authors: Michael S. Siddiqui, Atri Choksi, Christina D. Smolke Tags: Research Article Source Type: research
A system for multilocus chromosomal integration and transformation ‐free selection marker rescue
Abstract
Yeast integrating plasmids (YIPs) are a versatile tool for stable integration in Saccharomyces cerevisiae. However, current YIP systems necessitate time‐ and labor‐intensive methods for cloning and selection marker rescue. Here, we describe the design, construction, and validation of a new YIP system capable of accelerating the stable integration of multiple expression constructs into different loci in the yeast S. cerevisiae. These ‘directed pop‐out’ plasmids enable a simple, two‐step integration protocol that results in a scarless integration alongside a complete rescue of the selection marker. Thes...
Source: FEMS Yeast Research - October 9, 2014 Category: Research Authors: Michael S. Siddiqui, Atri Choksi, Christina D. Smolke Tags: Research Article Source Type: research
Functional characterization of dosage‐dependent lethal mutation of ubiquitin in Saccharomyces cerevisiae
Abstract
Ubiquitin is a eukaryotic protein with 96% sequence conservation from yeast to human. Ubiquitin plays a central role in protein homeostasis and regulation of protein function. We have reported on the generation of variants of ubiquitin by in vitro evolution in Saccharomyces cerevisiae to advance our understanding of the role of the invariant amino acid residues of ubiquitin in relation to its function. One of the mutants generated, namely UbEP42, was a dosage‐dependent lethal form of the ubiquitin gene, causing lethality to UBI4‐deficient cells but not to ubiquitin wild‐type cells. In the present study we in...
Source: FEMS Yeast Research - October 2, 2014 Category: Research Authors: Ankita Doshi, Pradeep Mishra, Mrinal Sharma, C. Ratna Prabha Tags: Research Article Source Type: research
Physiology of the fuel ethanol strain Saccharomyces cerevisiae PE ‐2 at low pH indicates a context‐dependent performance relevant for industrial applications
FEMS Yeast Research,Volume 14, Issue 8, Page 1196-1205, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Source Type: research
Role of asymmetric cell division in lifespan control in Saccharomyces cerevisiae
FEMS Yeast Research,Volume 14, Issue 8, Page 1133-1146, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Source Type: research
Detection and characterization of plasminogen receptors on clinical isolates of Trichosporon asahii
FEMS Yeast Research,Volume 14, Issue 8, Page 1186-1195, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Source Type: research
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FEMS Yeast Research,Volume 14, Issue 8, Page 1196-1205, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Source Type: research
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FEMS Yeast Research,Volume 14, Issue 8, Page 1133-1146, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Source Type: research
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FEMS Yeast Research,Volume 14, Issue 8, Page 1186-1195, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Source Type: research
Detection and characterization of plasminogen receptors on clinical isolates of Trichosporon asahii
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Authors: Reiko Ikeda, Tomoe Ichikawa, Yusuke Miyazaki, Nanaho Shimizu, Tomomi Ryoke, Kunihiko Haru, Takashi Sugita, Masako Takashima Tags: Research Paper Source Type: research
Role of asymmetric cell division in lifespan control in Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 26, 2014 Category: Research Authors: Ryo Higuchi‐Sanabria, Wolfgang M A Pernice, Jason D Vevea, Dana M. Alessi Wolken, Istvan R Boldogh, Liza A Pon Tags: MiniReview Source Type: research
The yeast oligopeptide transporter Opt2 is localized to peroxisomes and affects glutathione redox homeostasis
Abstract
Glutathione, the most abundant small‐molecule thiol in eukaryotic cells, is synthesized de novo solely in the cytosol and must subsequently be transported to other cellular compartments. The mechanisms of glutathione transport into and out of organelles remain largely unclear. We show that budding yeast Opt2, a close homolog of the plasma membrane glutathione transporter Opt1, localizes to peroxisomes. We demonstrate that deletion of OPT2 leads to major defects in maintaining peroxisomal, mitochondrial, and cytosolic glutathione redox homeostasis. Furthermore, ∆opt2 strains display synthetic lethality with del...
Source: FEMS Yeast Research - September 22, 2014 Category: Research Authors: Yael Elbaz‐Alon, Bruce Morgan, Anne Clancy, Theresa N.E. Amoako, Einat Zalckvar, Tobias P. Dick, Blanche Schwappach, Maya Schuldiner Tags: Research Article Source Type: research
Protection against cisplatin in calorie ‐restricted Saccharomyces cerevisiae is mediated by the nutrient‐sensor proteins Ras2, Tor1, or Sch9 through its target Glutathione
FEMS Yeast Research,Volume 14, Issue 8, Page 1147-1159, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 19, 2014 Category: Research Source Type: research
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FEMS Yeast Research,Volume 14, Issue 8, Page 1147-1159, December 2014. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 19, 2014 Category: Research Source Type: research
Application of synthetic biology for production of chemicals in yeast s. Cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - September 19, 2014 Category: Research Authors: Mingji Li, Irina Borodina Tags: MiniReview Source Type: research
