Applications of computational modeling in metabolic engineering of yeast
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - August 1, 2014 Category: Research Authors: Eduard J Kerkhoven, Petri‐Jaan Lahtvee, Jens Nielsen Tags: MiniReview Source Type: research
Genomic insights into the evolution of industrial yeast species Brettanomyces bruxellensis
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - August 1, 2014 Category: Research Authors: Christopher D. Curtin, Isak S. Pretorius Tags: MiniReview Source Type: research
Self‐excising integrative yeast plasmid vectors containing an intronated recombinase gene
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - August 1, 2014 Category: Research Authors: Michael Agaphonov, Alexander Alexandrov Tags: Research Paper Source Type: research
Yeast synthetic biology for the production of recombinant therapeutic proteins
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - August 1, 2014 Category: Research Authors: Hyunah Kim, Su Jin Yoo, Hyun Ah Kang Tags: MiniReview Source Type: research
A novel role of the ferric reductase Cfl1 in cell wall integrity, mitochondrial function and invasion to host cells in Candida albicans
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - August 1, 2014 Category: Research Authors: Qilin Yu, Yijie Dong, Ning Xu, Kefan Qian, Yulu Chen, Biao Zhang, Laijun Xing, Mingchun Li Tags: Research Paper Source Type: research
Cbc2p, Upf3p and eIF4G are components of the Degradation of mRNA in the Nucleus in Saccharomyces cerevisiae
In this study, we further identify three novel components of DRN (Cbc2p, Upf3p and Tif4631p) by employing a genetic screen and by considering proteins/factors that interact with Cbc1p. Participation of these components in DRN was confirmed by demonstrating that null alleles of these genes resulted in stabilization of the rapid decay of global mRNAs in the export defective nup116‐Δ strain and of representative special mRNAs. Depletion of Tif4632p, an isoform of Tif4631p, also exhibited a partial impairment of DRN function and is therefore also considered to play a functional role in DRN. These findings clearly establish ...
Source: FEMS Yeast Research - July 28, 2014 Category: Research Authors: Satarupa Das, Upasana Saha, Biswadip Das Tags: Research Article Source Type: research
Yeast synthetic biology for high‐value metabolites
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - July 22, 2014 Category: Research Authors: Zhubo Dai, Yi Liu, Juan Guo, Luqi Huang, Xueli zhang Tags: MiniReview Source Type: research
Multilocus sequence typing (MLST) and M13 PCR fingerprinting revealed heterogeneity amongst Cryptococcus species obtained from Italian veterinary isolates
Abstract
Cryptococcosis represents a fungal disease acquired from the environment with animals serving as host sentinels for human exposure. The aim of this study was to investigate the genetic characteristics of Cryptococcus isolates from veterinary sources (cats, dogs and birds) to understand their epidemiology and the genetic variability of the casual isolates. Mating‐type PCR in connection with MLST analysis using the ISHAM consensus MLST scheme for the C. neoformans/C. gattii species complex was used to genotype 17 C. neoformans isolates. In the absence of an MLST typing scheme Cryptococcus adeliensis, C. albidu...
Source: FEMS Yeast Research - July 21, 2014 Category: Research Authors: Patrizia Danesi, Carolina Firacative, Massimo Cogliati, Domenico Otranto, Gioia Capelli, Wieland Meyer Tags: Research Article Source Type: research
Recent applications of synthetic biology tools for yeast metabolic engineering
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - July 16, 2014 Category: Research Authors: Michael K.Jensen, Jay D. Keasling Tags: MiniReview Source Type: research
Cbc2p, Upf3p and eIF4G are components of the DRN (degradation of mRNA in the nucleus) in Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - July 7, 2014 Category: Research Authors: Satarupa Das, Upasana Saha, Biswadip Das Tags: Research Paper Source Type: research
Mechanosensitive channels Msy1 and Msy2 are required for maintaining organelle integrity upon hypoosmotic shock in fission yeast
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - July 7, 2014 Category: Research Authors: Yoshitaka Nakayama, Aiko Hirata, Hidetoshi Iida Tags: Short Communication Source Type: research
A newly identified fatty alcohol oxidase gene is mainly responsible for the oxidation of long‐chain ω‐hydroxy fatty acids in Yarrowia lipolytica
Abstract
Nine potential (fatty) alcohol dehydrogenase genes and one alcohol oxidase gene were identified in Yarrowia lipolytica by comparative sequence analysis. All relevant genes were deleted in Y. lipolytica H222ΔP which is lacking β‐oxidation. Resulting transformants were tested for their ability to accumulate ω‐hydroxy fatty acids and dicarboxylic acids in the culture medium. The deletion of eight alcohol dehydrogenase genes (FADH, ADH1‐7), which may be involved in ω‐oxidation, led only to a slightly increased accumulation of ω‐hydroxy fatty acids, whereas the deletion of the fatty alcohol oxidase gene...
Source: FEMS Yeast Research - July 2, 2014 Category: Research Authors: Michael Gatter, André Förster, Kati Bär, Miriam Winter, Christina Otto, Patrick Petzsch, Michaela Ježková, Katrin Bahr, Melanie Pfeiffer, Falk Matthäus, Gerold Barth Tags: Research Article Source Type: research
Multi‐Locus Sequence typing (MLST) and M13 PCR‐fingerprinting revealed heterogeneity amongst Cryptococcus species obtained from Italian veterinary isolates.
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - July 1, 2014 Category: Research Authors: P Danesi, C Firacative, M Cogliati, D Otranto, G Capelli, W Meyer Tags: Research Paper Source Type: research
l‐Dihydroxyphenylalanine induces melanin production by members of the genus Trichosporon
In this study, six strains, including two clinical isolates, from four different species (Trichosporon asahii, T. asteroides, T. inkin, and T. mucoides) were grown in culture media with or without l‐dihydroxyphenylalanine (l‐DOPA). Each strain produced a brownish pigment compatible with melanin when cultured in presence of l‐DOPA, suggesting that these species are able to produce eumelanin. l‐tyrosine was not able to elicit any type of pigment production on cultures. As eumelanin is produced by several fungi during parasitism, this pigment may contribute to Trichosporon virulence.
This work shows for the first ...
Source: FEMS Yeast Research - June 30, 2014 Category: Research Authors: Maria Helena Galdino Figueiredo‐Carvalho, Fábio B. Santos, Joshua D. Nosanchuk, Rosely M. Zancope‐Oliveira, Rodrigo Almeida‐Paes Tags: Short Communication Source Type: research
Major contribution of the Ehrlich pathway for 2‐phenylethanol/rose flavor production in Ashbya gossypii
Abstract
Aroma alcohols of fermented food and beverages are derived from fungal amino acids catabolism via the Ehrlich pathway. This linear pathway consists of three enzymatic reactions to form fusel alcohols. Regulation of some of the enzymes occurs on the transcriptional level via Aro80. The riboflavin overproducer Ashbya gossypii produces strong fruity flavours in contrast to its much less aromatic relative Eremothecium cymbalariae. Genome comparisons indicated that A. gossypii harbors genes for aromatic amino acid catabolism (ARO8a, ARO8b, ARO10, and ARO80) while E. cymbalariae only encodes ARO8a and thus lacks major...
Source: FEMS Yeast Research - June 27, 2014 Category: Research Authors: Davide Ravasio, Jürgen Wendland, Andrea Walther Tags: Research Article Source Type: research
