Cbc2p, Upf3p and eIF4G are components of the Degradation of mRNA in the Nucleus in Saccharomyces cerevisiae

In this study, we further identify three novel components of DRN (Cbc2p, Upf3p and Tif4631p) by employing a genetic screen and by considering proteins/factors that interact with Cbc1p. Participation of these components in DRN was confirmed by demonstrating that null alleles of these genes resulted in stabilization of the rapid decay of global mRNAs in the export defective nup116‐Δ strain and of representative special mRNAs. Depletion of Tif4632p, an isoform of Tif4631p, also exhibited a partial impairment of DRN function and is therefore also considered to play a functional role in DRN. These findings clearly establish that CBC2, UPF3, and TIF4631/32 gene products participate in DRN function.

Identification of novel components of DRN (Degradation of mRNA in the Nucleus), a nuclear mRNA quality control pathway in Saccharomyces cerevisiae.

Source: FEMS Yeast Research - July 28, 2014 Category: Research Authors: Satarupa Das, Upasana Saha, Biswadip Das Tags: Research Article Source Type: research