Issue Information
(Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 1, 2014 Category: Research Tags: Issue Information Source Type: research
Glucose‐ and nitrogen sensing and regulatory mechanisms in Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - April 17, 2014 Category: Research Authors: Steven V. Rødkær, Nils J. Færgeman Tags: MiniReview Source Type: research
The fraction of cells that resume growth after acetic acid addition is a strain‐dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae
Abstract
High acetic acid tolerance of Saccharomyces cerevisiae is a relevant phenotype in industrial biotechnology when using lignocellulosic hydrolysates as feedstock. A screening of 38 S. cerevisiae strains for tolerance to acetic acid revealed considerable differences, particularly with regard to the duration of the latency phase. To understand how this phenotype is quantitatively manifested, four strains exhibiting significant differences were studied in more detail. Our data show that the duration of the latency phase is primarily determined by the fraction of cells within the population that resume growth. Only thi...
Source: FEMS Yeast Research - April 11, 2014 Category: Research Authors: Steve Swinnen, Miguel Fernández‐Niño, Daniel González‐Ramos, Antonius J. A. Maris, Elke Nevoigt Tags: Research Article Source Type: research
Adenine auxotrophy – be aware: some effects of adenine auxotrophy in Saccharomyces cerevisiae strain W303‐1A
Abstract
Adenine auxotrophy is a commonly used genetic marker in haploid yeast strains. Strain W303‐1A, which carries the ade2‐1 mutation, is widely used in physiological and genetic research. Yeast extract‐based rich medium contains a low level of adenine, so that adenine is often depleted before glucose. This could affect the cell physiology of adenine auxotrophs grown in rich medium. The aim of our study was to assess the effects of adenine auxotrophy on cell morphology and stress physiology. Our results show that adenine depletion halts cell division, but that culture optical density continues to increase due to ...
Source: FEMS Yeast Research - April 11, 2014 Category: Research Authors: Agnese Kokina, Juris Kibilds, Janis Liepins Tags: Research Article Source Type: research
FK520 interacts with the discrete intrahelical amino acids of multidrug transporter Cdr1 protein and acts as antagonist to selectively chemosensitize azole‐resistant clinical isolates of Candida albicans
This study establishes the molecular basis of interaction of FK520 with Cdr1 protein, which is one of the major ABC multidrug transporters of C. albicans. For this, we have exploited an in‐house library of Cdr1 protein consisting of 252 mutant variants where the entire primary structure of the two transmembrane domains comprising of 12 transmembrane helices was subjected to alanine scanning. With these mutant variants of Cdr1 protein, we could identify the critical amino acids of the transporter protein, which if replaced with alanine, not only abrogated FK520‐dependent competitive inhibition of drug efflux but simult...
Source: FEMS Yeast Research - March 26, 2014 Category: Research Authors: Shweta Nim, Manpreet K. Rawal, Rajendra Prasad Tags: Research Article Source Type: research
Biodiversity of non‐Saccharomyces yeasts in distilleries of the La Mancha region (Spain)
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 21, 2014 Category: Research Authors: Juan Úbeda, María Maldonado Gil, Rosana Chiva, José M. Guillamón, Ana Briones Tags: Research Paper Source Type: research
Issue Information
(Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 17, 2014 Category: Research Tags: Issue Information Source Type: research
Quantitative phenotypic analysis of multistress response in Zygosaccharomyces rouxii complex
Abstract
Zygosaccharomyces rouxii complex comprises three yeasts clusters sourced from sugar‐ and salt‐rich environments: haploid Zygosaccharomyces rouxii, diploid Zygosaccharomyces sapae and allodiploid/aneuploid strains of uncertain taxonomic affiliations. These yeasts have been characterized with respect to gene copy number variation, karyotype variability and change in ploidy, but functional diversity in stress responses has not been explored yet. Here, we quantitatively analysed the stress response variation in seven strains of the Z. rouxii complex by modelling growth variables via model and model‐free fitting...
Source: FEMS Yeast Research - March 13, 2014 Category: Research Authors: Lisa Solieri, Tikam C. Dakal, Silvio Bicciato Tags: Research Article Source Type: research
Three Agt1 transporters from brewer's yeasts exhibit different temperature dependencies for maltose transport over the range of brewery temperatures (0–20 °C)
Abstract
Zero‐trans rates of maltose transport by brewer's yeasts exert strong control over fermentation rates and are strongly temperature‐dependent over the temperature range (20–0 °C) of brewery fermentations. Three α‐glucoside transporters, ScAgt1(A60) (a Saccharomyces cerevisiae version of Agt1 from an ale strain), ScAgt1‐A548V (a variant of ScAgt1(A60) with a single amino acid change in a transmembrane domain), and SbAgt1 (a Saccharomyces (eu)bayanus version from a lager strain), were compared. When expressed in the same laboratory yeast, grown at 24 °C and assayed at 0, 10, and 20 °C, SbAgt1 had the...
Source: FEMS Yeast Research - March 11, 2014 Category: Research Authors: Virve Vidgren, Kaarina Viljanen, Laura Mattinen, Jari Rautio, John Londesborough Tags: Research Article Source Type: research
Regulation of Saccharomyces cerevisiae MEF1 by Hda1p affects salt resistance of bdf1Δ mutant
In this study, we reported that the salt sensitivity, mitochondrial dysfunction, and nuclear instability of bdf1Δ mutant were suppressed by HDA1 deletion or MEF1 overexpression. Hda1p overexpression inhibited the relieving effects of low‐copy overexpression of MEF1. Further analysis showed that Bdf1p regulated HDA1 transcription positively by binding to its promoter at −201 to +6 bp, whereas Hda1p modulated MEF1 expression negatively by binding to its promoter at −201 to +6 bp. These results suggested that Bdf1p likely regulated MEF1 expression negatively by regulating HDA1 positively. Mitochondrial proteomics ana...
Source: FEMS Yeast Research - March 11, 2014 Category: Research Authors: Lei Chen, Mingpeng Wang, Jin Hou, Liangyu Liu, Jiafang Fu, Yu Shen, Zhaojie Zhang, Xiaoming Bao Tags: Research Article Source Type: research
The Influence Of Wickerhamomyces Anomalus Killer Yeast On The Fermentation And Chemical Composition Of Apple Wines
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 1, 2014 Category: Research Authors: Pawel Satora, Tomasz Tarko, Pawel Sroka, Urszula Blaszczyk Tags: Research Paper Source Type: research
Marine Yeast Isolation and Industrial Application
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 1, 2014 Category: Research Authors: Abdelrahman Saleh Zaky, Gregory A. Tucker, Zakaria Yehia Daw, Chenyu Du Tags: MiniReview Source Type: research
Microarray studies on lager brewer's yeasts reveal cell status in the process of autolysis
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 1, 2014 Category: Research Authors: Weina Xu, Jinjing Wang, Qi Li Tags: Research Paper Source Type: research
Adaptation of Candida albicans to growth on sorbose via monosomy of chromosome 5 accompanied by duplication of another chromosome carrying a gene responsible for sorbose utilization
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 1, 2014 Category: Research Authors: Anatoliy Kravets, Feng Yang, Gabor Bethlendy, Fred Sherman, Elena Rustchenko Tags: Research Paper Source Type: research
Adenine auxotrophy – be aware. Some effects of adenine auxotrophy in Saccharomyces cerevisiae strain W303‐1A
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - March 1, 2014 Category: Research Authors: Agnese Kokina, Juris Kibilds, Janis Liepins Tags: Research Paper Source Type: research
