Primers for fourteen protein‐coding genes and the deep phylogeny of the true yeasts
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - June 20, 2013 Category: Research Authors: Vassiliki Koufopanou, Jonathan Swire, Susan Lomas, Austin Burt Tags: Research Paper Source Type: research
Pyruvate decarboxylase activity is regulated by the Ser/Thr protein phosphatase Sit4p in the yeast Saccharomyces cerevisiae
Abstract
Deletion of SIT4 phosphatase decreased the pyruvate decarboxylase activity, which is essential for directing the glucose flux to ethanol production. Concomitantly, a reduction in the fermentative capacity was observed. As pyruvate decarboxylase expression was not altered, its post‐translational phosphorylation was studied. Immunoblot analyses using anti‐phosphoserine antibodies against the affinity‐purified Pdc1p showed that Pdc1p is a phosphoenzyme. Dephosphorylation of Pdc1p by alkaline phosphatase inhibited activity by 50%. Moreover, phosphorylation of Pdc1p was dependent on the growth phase, being hyperp...
Source: FEMS Yeast Research - June 18, 2013 Category: Research Authors: Leandro José Assis, Russolina Benedeta Zingali, Claudio Akio Masuda, Silas Pessini Rodrigues, Monica Montero‐Lomelí Tags: Research Article Source Type: research
Functional improvement of Saccharomyces cerevisiae to reduce volatile acidity in wine
In this study, we investigated the production of VA by a deletion mutant of the fermentation stress response gene AAF1 in the budding yeast Saccharomyces cerevisiae. Fermentations were carried out in commercial Chardonnay grape must to mimic industrial wine‐making conditions. We demonstrated that a wine yeast strain deleted for AAF1 reduced acetic acid levels in wine by up to 39.2% without increasing the acetaldehyde levels, revealing a potential for industrial application. Deletion of the cytosolic aldehyde dehydrogenase gene ALD6 also reduced acetic acid levels dramatically, but increased the acetaldehyde levels by 41....
Source: FEMS Yeast Research - June 13, 2013 Category: Research Authors: Zongli Luo, Christopher J. Walkey, Lufiani L. Madilao, Vivien Measday, Hennie J.J. Vuuren Tags: Research Article Source Type: research
Pichia anomala 29X: A resistant strain for lignocellulosic biomass hydrolysate fermentation
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - June 1, 2013 Category: Research Authors: Ying Zha, Abeer H. Hossain, Felix Tobola, Norbert Sedee, Mieke Havekes, Peter J. Punt Tags: Research Paper Source Type: research
Phylogenetic and phenotypic characterisation of the 3‐ketoacyl‐CoA thiolase gene family from the opportunistic human pathogenic fungus Candida albicans
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - June 1, 2013 Category: Research Authors: Christian Otzen, Sebastian Müller, Ilse D. Jacobsen, Matthias Brock Tags: Research Paper Source Type: research
Histone biotinylation in Candida albicans
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 29, 2013 Category: Research Authors: Sahar Hasim, Swetha Tati, Nandakumar Madayiputhiya, Renu Nandakumar, Kenneth W. Nickerson Tags: Research Paper Source Type: research
Revised manuscript FEMSYR‐13‐01‐0002 without highlighted corrections Selection of Botrytis cinerea and Saccharomyces cerevisiae strains for the improvement and valorization of Italian passito style wines
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 24, 2013 Category: Research Authors: Michela Azzolini, Emanuele Tosi, Stefano Faccio, Marilinda Lorenzini, Sandra Torriani, Giacomo Zapparoli Tags: Research Paper Source Type: research
Characterization of fungal RTG2 genes in retrograde signaling of Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 24, 2013 Category: Research Authors: Ercan Selçuk Ünlü, Lakshmi Narayanan, Donna M. Gordon Tags: Research Paper Source Type: research
Functional analysis and transcriptional regulation of two orthologs of ARO10, encoding broad‐substrate‐specificity 2‐oxo‐acid decarboxylases, in the brewing yeast Saccharomyces pastorianus CBS1483
This study focuses on ARO10, a 2 oxo‐acid decarboxylase involved in production of higher alcohols. In S.pastorianus CBS1483, four ARO10 copies were identified, three resembled S.cerevisiae ARO10 and one S. eubayanus ARO10. Substrate specificities of lager strain (Lg)ScAro10 and LgSeubAro10 were compared by individually expressing them in a pdc1Δ‐pdc5Δ‐pdc6Δ‐aro10Δ‐thi3Δ S. cerevisiae strain. Both isoenzymes catalysed decarboxylation of the 2‐oxo‐acids derived from branched‐chain, sulphur‐containing amino‐acids isoleucine, and preferably phenylpyruvate. Expression of both alleles was induced by phen...
Source: FEMS Yeast Research - May 21, 2013 Category: Research Authors: Irina Bolat, Gabriele Romagnoli, Feibai Zhu, Jack T. Pronk, Jean‐Marc Daran Tags: Research Paper Source Type: research
Pyruvate decarboxylase activity is regulated by the Ser/Thr protein phosphatase SIT4 in the yeast Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 21, 2013 Category: Research Authors: Leandro José Assis, Russolina Benedeta Zingali, Claudio Akio Masuda, Silas Pessini Rodrigues, Monica Montero Lomeli Tags: Research Paper Source Type: research
Functional improvement of S. cerevisiae to reduce volatile acidity in wine
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 21, 2013 Category: Research Authors: Zongli Luo, Christopher J. Walkey, Lufiani L. Madilao, Vivien Measday, Hennie J. J. Vuuren Tags: Research Paper Source Type: research
Characterization of an M28 metalloprotease family member residing in the yeast vacuole
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 20, 2013 Category: Research Authors: Karen A. Hecht, Victoria A. Wytiaz, Tslil Ast, Maya Schuldiner, Jeffrey L. Brodsky Tags: Research Paper Source Type: research
Adaptation of Saccharomyces cerevisiae to Toxic Manganese Concentration Triggers Changes in Inorganic Polyphosphates
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - May 11, 2013 Category: Research Authors: Nadezhda Andreeva, Lubov Ryazanova, Vladimir Dmitriev, Tatiana Kulakovskaya, Igor Kulaev Tags: Research Paper Source Type: research
Synergistic effect of doxycycline and fluconazole against Candida albicans biofilms and the impact of calcium channel blockers
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - April 14, 2013 Category: Research Authors: Yuan Gao, Caiqing Zhang, Chunyan Lu, Ping Liu, Yan Li, Hui Li, Shujuan Sun Tags: Research Paper Source Type: research
Comparative metabolic footprinting of a large number of commercial wine yeast strains in Chardonnay fermentations
Abstract
Wine has been made for thousands of years. In modern times, as the importance of yeast as an ingredient in winemaking became better appreciated, companies worldwide have collected and marketed specific yeast strains for enhancing positive and minimizing negative attributes in wine. It is generally believed that each yeast strain contributes uniquely to fermentation performance and wine style because of its genetic background; however, the impact of metabolic diversity among wine yeasts on aroma compound production has not been extensively studied. We characterized the metabolic footprints of 69 different commercia...
Source: FEMS Yeast Research - April 12, 2013 Category: Research Authors: Chandra L. Richter, Barbara Dunn, Gavin Sherlock, Tom Pugh Tags: Research Article Source Type: research
