Not your ordinary yeast: non‐Saccharomyces yeasts in wine production uncovered
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 24, 2013 Category: Research Authors: Neil P. Jolly, Cristian Varela, Isak S. Pretorius Tags: MiniReview Source Type: research
Approaches to study yeast cell aging and death
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 24, 2013 Category: Research Authors: Mario G. Mirisola, Ralf J. Braun, Dina Petranovic Tags: MiniReview Source Type: research
Negative control glucose dependent mediated by the PreS2 region on the translation efficiency of the reporter Sh‐bleomycin gene in Saccharomycess cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 23, 2013 Category: Research Authors: Nadia Hadiji‐Abbes, Istabrak Borchani‐Chabchoub, Ali Gargouri, Raja Mokdad‐Gargouri Tags: Research Paper Source Type: research
EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 23, 2013 Category: Research Authors: N. B. Jensen, T. Strucko, K. R. Kildegaard, F. David, J. Maury, U. H. Mortensen, J. Förster, J. B. Nielsen, I. Borodina Tags: Research Paper Source Type: research
Actin – a biosensor that determines cell fate in yeasts
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 23, 2013 Category: Research Authors: Daniel G.J. Smethurst, Ian W. Dawes, Campbell W. Gourlay Tags: MiniReview Source Type: research
Improved sake metabolic profile during fermentation due to increased mitochondrial pyruvate dissimilation
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 23, 2013 Category: Research Authors: Gennaro Agrimi, Maria C. Mena, Kazuki Izumi, Isabella Pisano, Lucrezia Germinario, Hisashi Fukuzaki, Luigi Palmieri, Lars M. Blank, Hiroshi Kitagaki Tags: Research Paper Source Type: research
Molecular mechanisms linking the evolutionary conserved TORC1–Sch9 nutrient signalling branch to lifespan regulation in Saccharomyces cerevisiae
Abstract
The knowledge on the molecular aspects regulating ageing in eukaryotic organisms has benefitted greatly from studies using the budding yeast Saccharomyces cerevisiae. Indeed, many aspects involved in the control of lifespan appear to be well conserved among species. Of these, the lifespan‐extending effects of calorie restriction (CR) and downregulation of nutrient signalling through the target of rapamycin (TOR) pathway are prime examples. Here, we present an overview on the molecular mechanisms by which these interventions mediate lifespan extension in yeast. Several models have been proposed in the literature,...
Source: FEMS Yeast Research - October 11, 2013 Category: Research Authors: Erwin Swinnen, Ruben Ghillebert, Tobias Wilms, Joris Winderickx Tags: MiniReview Source Type: research
GAL regulon of Saccharomyces cerevisiae performs optimally to maximize growth on galactose
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 11, 2013 Category: Research Authors: Pushkar Malakar, K.V. Venkatesh Tags: Research Paper Source Type: research
Incidence of symbiotic dsRNA ‘killer’ viruses in wild and domesticated yeast
Abstract
Viruses are found in almost all organisms and physical habitats. One interesting example is the yeast viral ‘killer system’. The virus provides the host with a toxin directed against strains that do not carry it, while the yeast cell enables its propagation. Although yeast viruses are believed to be common, they have been actually described only for a limited number of yeast isolates. We surveyed 136 Saccharomyces cerevisiae and S. paradoxus strains of known origin and phylogenetic relatedness. Of these, 14 (c. 10%) were infected by killer viruses of one of the three types: K1, K2 or K28. As many as 34 strain...
Source: FEMS Yeast Research - October 7, 2013 Category: Research Authors: Magdalena D. Pieczynska, J. Arjan G. M. Visser, Ryszard Korona Tags: Short Communication Source Type: research
Reactive oxygen species production induced by ethanol in Saccharomyces cerevisiae increases because of a dysfunctional mitochondrial iron–sulfur cluster assembly system
Abstract
Ethanol accumulation during fermentation contributes to the toxic effects in Saccharomyces cerevisiae, impairing its viability and fermentative capabilities. The iron–sulfur (Fe–S) cluster biogenesis is encoded by the ISC genes. Reactive oxygen species (ROS) generation is associated with iron release from Fe–S‐containing enzymes. We evaluated ethanol toxicity, ROS generation, antioxidant response and mitochondrial integrity in S. cerevisiae ISC mutants. These mutants showed an impaired tolerance to ethanol. ROS generation increased substantially when ethanol accumulated at toxic concentrations under the f...
Source: FEMS Yeast Research - October 7, 2013 Category: Research Authors: Rocio V. Pérez‐Gallardo, Luis S. Briones, Alma L. Díaz‐Pérez, Sergio Gutiérrez, José S. Rodríguez‐Zavala, Jesús Campos‐García Tags: Research Article Source Type: research
Alcohols are inhibitors of Saccharomyces cerevisiae multidrug‐resistance pumps Pdr5p and Snq2p
Abstract
The effect of alcohols on cell membrane proteins has originally been assumed to be mediated by their primary action on membrane lipid matrix. Many studies carried out later on both animal and yeast cells have revealed that ethanol and other alcohols inhibit the functions of various membrane channels, receptors and solute transport proteins, and a direct interaction of alcohols with these membrane proteins has been proposed. Using our fluorescence diS‐C3(3) diagnostic assay for multidrug‐resistance pump inhibitors in a set of isogenic yeast Pdr5p and Snq2p mutants, we found that n‐alcohols (from ethanol to he...
Source: FEMS Yeast Research - October 7, 2013 Category: Research Authors: Dana Gášková, Jaromír Plášek, Jakub Zahumenský, Ivana Benešová, Ľuboslava Buriánková, Karel Sigler Tags: Research Article Source Type: research
One‐step assembly and targeted integration of multigene constructs assisted by the I‐SceI meganuclease in Saccharomyces cerevisiae
This study paves the way for the fast, efficient, and stable integration of large DNA constructs in S. cerevisiae chromosomes. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 7, 2013 Category: Research Authors: Niels G.A. Kuijpers, Soultana Chroumpi, Tim Vos, Daniel Solis‐Escalante, Lizanne Bosman, Jack T. Pronk, Jean‐Marc Daran, Pascale Daran‐Lapujade Tags: Research Article Source Type: research
The MX4blaster cassette: repeated and clean Saccharomyces cerevisiae genome modification using the genome‐wide deletion collection
Abstract
The kanMX4 resistance marker is widely used for Saccharomyces cerevisiae gene deletion and has been used to create a genome‐wide deletion mutant collection. Transfer of PCR‐amplified marker loci from collection mutants is a very efficient way of introducing mutations into other S. cerevisiae strains of interest. An important limitation of this strategy is that the kanMX4 marker is not easily removed, impairing the construction of multiple gene deletion strains. The MX4blaster is a novel MX4‐compatible cassette that facilitates clean or scarless kanMX4 removal. The MX4blaster cassette efficiently replaces th...
Source: FEMS Yeast Research - October 7, 2013 Category: Research Authors: Ângela Carvalho, Filipa Pereira, Björn Johansson Tags: Research Article Source Type: research
Squalene epoxidase as a target for manipulation of squalene levels in the yeast Saccharomyces cerevisiae
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 7, 2013 Category: Research Authors: Martina GaraiováZ, Veronika Zambojová, Zuzana Šimová, Peter Griač, Ivan Hapala Tags: Research Paper Source Type: research
Yeast replicative aging: A paradigm for defining conserved longevity interventions
This article is protected by copyright. All rights reserved. (Source: FEMS Yeast Research)
Source: FEMS Yeast Research - October 3, 2013 Category: Research Authors: Brian M. Wasko, Matt Kaeberlein Tags: MiniReview Source Type: research
