Transformation of Candida guilliermondii wild‐type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin‐resistant marker

Abstract We designed an efficient transformation system for C. guilliermondii wild‐type strains. We demonstrated that the Staphylococcus aureus MRSA 252 ble coding sequence placed under the control of the yeast phosphoglycerate kinase gene transcription‐regulating regions confers phleomycin resistance to transformed C. guilliermondii cells. To illustrate the potential of this drug‐resistant cassette, we carried out the disruption of the C. guilliermondii ADE2 gene. This new dominant selectable marker represents a powerful tool to study the function of various genes in this yeast of clinical and biotechnological interest. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
Source: FEMS Yeast Research - Category: Research Authors: Tags: Short Communication Source Type: research