Transcriptional profiling of Brazilian Saccharomyces cerevisiae strains selected for semi‐continuous fermentation of sugarcane must

This study presents a genome wide analysis of the CAT‐1 and PE‐2 transcriptomes during a small‐scale fermentation process that mimicked the industrial conditions. The common and unique transcriptional responses of the two strains to the Brazilian fermentation process were identified. Environmental stress response genes were up regulated post fermenter feeding, demonstrating the impact of the prior acid wash and high glucose environment. Cell wall and oxidative stress tolerance were subsequently demonstrated to be enhanced for the industrial strains. Conversely, numerous genes involved in protein synthesis were down regulated at the end of fermentation revealing the later impact of ethanol induced stress. Subsequently, the industrial strains demonstrated a greater tolerance of ethanol and the disruption of endoplasmic reticulum homeostasis. This increased ethanol tolerance was finally correlated with an increased unfolded protein response and increased HAC1 splicing. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
Source: FEMS Yeast Research - Category: Research Authors: Tags: Research Paper Source Type: research