Development, Growth and Differentiation This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Adenosine Triphosphate stimulates differentiation and mineralization in human osteoblast‐like Saos‐2 cells
In conclusion, we show the osteogenic effect of ATP on both early and late stage activities like differentiation and mineralization, for the first time in human osteoblastic cells. In osteoblasts ATP can regulate several activities like cell proliferation, cell death, cell differentiation and matrix mineralization. ATP can increase alkaline phosphatase (ALP) activity in a dose dependent manner as well as it can reduce cell proliferation in human osteoblast‐like Saos‐2 cells. ATP can affect mineralization in a biphasic manner inducing an increase of Calcium deposits at lower concentrations and a reduction of mineral de...
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Authors: Alessandro Cutarelli, Mario Marini, Virginia Tancredi, Giovanna D'Arcangelo, Michela Murdocca, Claudio Frank, Umberto Tarantino Tags: Original Article Source Type: research

ADAM12‐deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects
In this study, we used a simple model vertebrate, the zebrafish, to investigate the functions of ADAM12 during development. Zebrafish adam12 is conserved with those of mammals in the synteny and the amino‐acid sequence. We examined adam12 expression in zebrafish embryos by whole mount in situ hybridization and the promoter activity of the adam12 upstream sequence. We found that adam12 is strongly expressed in the cardiovascular system, erythroid progenitors, brain, and jaw cartilage during zebrafish development, and adam12‐knockout zebrafish exhibited reduced body size in the juvenile stage without apparent morphologi...
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Authors: Yudai Tokumasu, Atsuo Iida, Zi Wang, Satoshi Ansai, Masato Kinoshita, Atsuko Sehara‐Fujisawa Tags: Original Article Source Type: research

Regulation of the neural niche by the soluble molecule Akhirin
Though the adult central nervous system has been considered a comparatively static tissue with little turnover, it is well established today that new neural cells are generated throughout life. Neural stem/progenitor cells (NS/PCs) can self‐renew and generate all types of neural cells. The proliferation of NS/PCs, and differentiation and fate determination of PCs are regulated by extrinsic factors such as growth factors, neurotrophins, and morphogens. Although several extrinsic factors that influence neurogenesis have already been reported, little is known about the role of soluble molecules in neural niche regulation. I...
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Authors: Uzzal Kumar Acharjee, Athary Abdulhaleem Felemban, Asrafuzzaman M. Riyadh, Kunimasa Ohta Tags: Review Article Source Type: research

Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice
Some organisms, such as zebrafish, urodele amphibians, and newborn mice, have a capacity for heart regeneration following injury. However, adult mammals fail to regenerate their hearts. To know why newborn mice can regenerate their hearts, we focused on epigenetic factors, which are involved in cell differentiation in many tissues. Baf60c (BRG1/BRM‐associated factor 60c), a component of ATP‐dependent chromatin‐remodeling complexes, has an essential role for cardiomyocyte differentiation at the early heart development. To address the function of Baf60c in postnatal heart homeostasis and regeneration, we examined the d...
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Authors: Ryo Nakamura, Kazuko Koshiba‐Takeuchi, Megumi Tsuchiya, Mizuyo Kojima, Asuka Miyazawa, Kohei Ito, Hidesato Ogawa, Jun K. Takeuchi Tags: Original Article Source Type: research

Modeling the autistic cell: iPSCs recapitulate developmental principles of syndromic and nonsyndromic ASD
The opportunity to model autism spectrum disorders (ASD) through generation of patient‐derived induced pluripotent stem cells (iPSCs) is currently an emerging topic. Wide‐scale research of altered brain circuits in syndromic ASD, including Rett Syndrome, Fragile X Syndrome, Angelman's Syndrome and sporadic Schizophrenia, was made possible through animal models. However, possibly due to species differences, and to the possible contribution of epigenetics in the pathophysiology of these diseases, animal models fail to recapitulate many aspects of ASD. With the advent of iPSCs technology, 3D cultures of patient‐derived ...
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Authors: Lihi Ben‐Reuven, Orly Reiner Tags: Review Article Source Type: research

Mature neurons modulate neurogenesis through chemical signals acting on neural stem cells
The discovery of neural stem cells has revealed a much higher structural and functional plasticity in the adult nervous system than previously anticipated. Progenitor cells are able to give rise to new neurons and glial cells when needed, thanks to their surveillance of the environment from the germinal niches. Multiple different factors define neural stem cell niches, including cellular and non‐cellular components. Innervation of neurogenic centers is crucial, as it allows the functional connection between stem cell behavior and surrounding neuronal activity. Although the association between organismal behavior and neur...
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Authors: Ricardo Pardal, José López Barneo Tags: Review Article Source Type: research

Issue Information
Cover Photograph: An sea urchin embryo of Hemicentrotus pulcherris injected with synthetic mRNA for Micro1 (a paired‐like class homeobox gene) and stained with anti P4 antibody. Almost all cells expressed skeletogenic cell‐specific antigen P4. See Yamazaki & Minokawa (pp. 315–326). Article link here (Source: Development, Growth and Differentiation)
Source: Development, Growth and Differentiation - March 31, 2016 Category: Research Tags: Issue Information Source Type: research

Maturation promoting factor destabilization facilitates postovulatory aging‐mediated abortive spontaneous egg activation in rat
The present study was designed to investigate whether destabilization of maturation promoting factor (MPF) leads to postovulatory aging‐mediated abortive spontaneous egg activation (SEA). If so, we wished to determine whether changes in Wee‐1 as well as Emi2 levels are associated with MPF destabilization during postovulatory aging‐mediated abortive SEA in rats eggs aged in vivo. For this purpose, sexually immature female rats were given a single injection (20 IU IM) of pregnant mare serum gonadotropin for 48 h followed by single injection of human chorionic gonadotropin (20 IU). Ovulated eggs were collected after ...
Source: Development, Growth and Differentiation - March 17, 2016 Category: Research Authors: Shilpa Prasad, Biplob Koch, Shail K. Chaube Tags: Original Article Source Type: research

Blastocysts derivation from somatic cell fusion with premature oocytes (prematuration somatic cell fusion)
This study was undertaken to investigate the development of immature oocytes after their fusion with male somatic cells expressing red fluorescence protein (RFP). RFP‐expressing cells were fused with immature oocytes, matured in vitro and then parthenogenetically activated. Somatic nuclei showed spindle formation, 1st polar body extrusion after in vitro maturation and protruded the 2nd polar body after parthenogenetic activation. RFP was expressed in the resultant embryos; two‐cell stage and blastocysts. Chromosomal analysis showed aneuploidy in 81.82% of the resulting blastocysts while 18.18% of the resulting blasto...
Source: Development, Growth and Differentiation - February 9, 2016 Category: Research Authors: Islam M. Saadeldin, Candrani Khoirinaya, Su Jin Kim, Joon Ho Moon, Essam Almadaly, Byeong Chun Lee Tags: Original Article Source Type: research

Heterogeneity of chromatoid bodies in adult pluripotent stem cells of planarian Dugesia japonica
The robust regenerative ability of planarians is known to be dependent on adult pluripotent stem cells called neoblasts. One of the morphological features of neoblasts is cytoplasmic ribonucleoprotein granules (chromatoid bodies: CBs), which resemble germ granules present in germline cells in other animals. Previously, we showed by immuno‐electron microscopic analysis that DjCBC‐1, a planarian Me31B/Dhh1/DDX6 homologue, which is a component of ribonucleoprotein granules, was localized in CBs in the planarian Dugesia japonica. Also, recently it was reported using another planarian species that Y12 antibody recognizing s...
Source: Development, Growth and Differentiation - February 9, 2016 Category: Research Authors: Makoto Kashima, Nobuyoshi Kumagai, Kiyokazu Agata, Norito Shibata Tags: Original Article Source Type: research

Embryos aggregation improves development and imprinting gene expression in mouse parthenogenesis
In conclusion, four embryos aggregation improves parthenogenetic development, and compensates imprinting genes expression in PgESCs. It implied that a4PgESCs could serve as a better scientific model applied in translational medicine and imprinting gene study. In mouse parthenogenesis, four embryos aggregation could be improve the implantation development and compensate imprinting gene expression. (Source: Development, Growth and Differentiation)
Source: Development, Growth and Differentiation - February 1, 2016 Category: Research Authors: Guang‐Yu Bai, Si‐Hang Song, Zhen‐Dong Wang, Zhi‐Yan Shan, Rui‐Zhen Sun, Chun‐Jia Liu, Yan‐Shuang Wu, Tong Li, Lei Lei Tags: Original Article Source Type: research

Simple blood‐feeding method for live imaging of gut tube remodeling in regenerating planarians
Live cell imaging is a powerful technique to study cellular dynamics in vivo during animal development and regeneration. However, few live imaging methods have been reported for studying planarian regeneration. Here, we developed a simple method for steady visualization of gut tube remodeling during regeneration of a living freshwater planarian, Dugesia japonica. When planarians were fed blood several times, gut branches were well‐visualized in living intact animals under normal bright‐field illumination. Interestingly, tail fragments derived from these colored planarians enabled successive observation of the processe...
Source: Development, Growth and Differentiation - February 1, 2016 Category: Research Authors: Kazutaka Hosoda, Mizuki Morimoto, Minako Motoishi, Osamu Nishimura, Kiyokazu Agata, Yoshihiko Umesono Tags: Original Article Source Type: research

Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase
The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho‐specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase act...
Source: Development, Growth and Differentiation - February 1, 2016 Category: Research Authors: Stephen A. Stricker, Bradley Beckstrom, Cristina Mendoza, Emma Stanislawski, Tewodros Wodajo Tags: Original Article Source Type: research

Issue Information
(Source: Development, Growth and Differentiation)
Source: Development, Growth and Differentiation - February 1, 2016 Category: Research Tags: Issue Information Source Type: research

Efficient harvesting methods for early‐stage snake and turtle embryos
Reptile development is an intriguing research target for understating the unique morphogenesis of reptiles as well as the evolution of vertebrates. However, there are numerous difficulties associated with studying development in reptiles. The number of available reptile eggs is usually quite limited. In addition, the reptile embryo is tightly adhered to the eggshell, making it a challenge to isolate reptile embryos intact. Furthermore, there have been few reports describing efficient procedures for isolating intact embryos especially prior to pharyngula stage. Thus, the aim of this review is to present efficient procedures...
Source: Development, Growth and Differentiation - January 31, 2016 Category: Research Authors: Yoshiyuki Matsubara, Atsushi Kuroiwa, Takayuki Suzuki Tags: Review Article Source Type: research