Progress on Detection of Metals Ions by Functional Nucleic Acids Biosensor
Publication date: July 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 7Author(s): Zai-Hui DU, Xiang-Yang LI, Jing-Jing TIAN, Yang-Zi Zhang, Hong-Tao TIAN, Wen-Tao XUAbstractFunctional nucleic acids are natural or artificial nucleic acid sequences with specific functions and special structures. A part of metal ions are essential trace elements of human health, but excessive metal ions will be harmful to human health. The functional nucleic acids are widely used for detection of metal ions because of its advantages such as easy modification, low price, high stability and strong specificity. This paper ...
Source: Chinese Journal of Analytical Chemistry - July 6, 2018 Category: Chemistry Source Type: research

Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications
Publication date: July 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 7Author(s): Chang-Fang SHAO, Yao ZHAO, Kui WU, Fei-Fei JIA, Qun LUO, Zhe LIU, Fu-Yi WANGAbstractSecondary ion mass spectrometry (SIMS) as a powerful surface analysis technique has been widely applied in semiconductor industry and geology research. Recently, with the development of instrumental technology, SIMS is attracting more and more attention in life sciences. SIMS can provide surface MS spectra, 2D/3D chemical images and depth profiling of substances simultaneously. The minimal lateral resolution of 2D SIMS imaging is 80–10...
Source: Chinese Journal of Analytical Chemistry - July 6, 2018 Category: Chemistry Source Type: research

Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
Publication date: July 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 7Author(s): Xin-Yu HE, Bing WANG, Yang-Yang ZHOU, Xiao-Jun BIAN, Juan YANAbstractAs a low molecular weight protein with the ability of binding metal ions and high inducibility, metallothionein (MT) is often regarded as an important biomarker for assessment of heavy metal pollution in water environment. In the light of that the traditional process of enrichment and identification is time-consuming and complicated, we prepared a core-shell nanoparticle, gold-coated iron oxide nanoparticles (Fe3O4@Au NPs) herein. It possessed the adva...
Source: Chinese Journal of Analytical Chemistry - July 6, 2018 Category: Chemistry Source Type: research

Solid-phase Extraction for Analysis of Short-Chain Chlorinated Paraffins in Water Samples
Publication date: July 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 7Author(s): Ying-Jun WANG, Ya-Wei WANG, Gui-Bin JIANGAbstractA method for analysis of short-chain chlorinated paraffins (SCCPs) in water based on solid-phase extraction (SPE) was established using electron capture negative chemical ionization mass spectrometry (GC-ENCI-MS). The SPE parameters, including the sorbent, eluent and elution volume were optimized. The Agilent Bond Elut-C18 was precleaned with 3 mL of hexane and 3 mL of methanol, and subsequently conditioned with 3 mL H2O prior to use. After the water samples were enriched...
Source: Chinese Journal of Analytical Chemistry - July 6, 2018 Category: Chemistry Source Type: research

Quantitative Detection of PremicroRNA-21 Based on Chimeric Molecular Beacon
Publication date: July 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 7Author(s): Hai-Long XIE, Tian-Tian WU, Zhen-Hua XIE, Jia-Long FAN, Chun-Yi TONGAbstractAs an important tumor marker, the expression level of premicroRNA-21 (pre-miR-21) can provide important information for early diagnosis, drug treatment and prognosis of tumor. Thus, developing pre-miR-21 detection is of great importance for diagnosis of disease. Herein, a direct, simple and rapid method for quantitative detection of pre-miR-21 was developed. This detecting system contained the tool of molecular beacon which could hybridize speci...
Source: Chinese Journal of Analytical Chemistry - July 6, 2018 Category: Chemistry Source Type: research

Ethanol Gas Sensor Based on γ-Fe2O3 Nanoparticles Working at Room Temperature with High Sensitivity
This study indicated that the γ-Fe2O3 phase could be a type of promising room-temperature gas sensing material for ethanol gas detection.Graphical AbstractIn the present work, γ-Fe2O3 nanoparticles were obtained by an annealing treatment. The gas-sensing experiments showed that the γ-Fe2O3 nanoparticles displayed excellent high-performance sensing properties for the detection of ethanol gas at the optimum operating temperature (200 °C) and room temperature. This study indicates that γ-Fe2O3 nanoparticles could be a type of promising gas sensing material for ethanol gas detection. (Source: Chinese Journal of Analytical Chemistry)
Source: Chinese Journal of Analytical Chemistry - July 6, 2018 Category: Chemistry Source Type: research

Development of Microchip Electrophoresis and Its Applications in Ion Detection
Publication date: May 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 5Author(s): Ming-Peng YANG, Zhe HUANG, Yang XIE, Hui YOUAbstractMicrochip electrophoresis is emerging as a highly promising method due to its portability and fast analysis with a minimum number of analytes. In this review, recent developments in microchip electrophoresis were described with discussions on the structures, materials, processing technologies, surface modification, experimental methods, and applications in ion detection. The existing issues and prospects of future studies were discussed as well.Graphical abstractMicroch...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Separation and Identification of Microplastics in Digestive System of Bivalves
Publication date: May 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 5Author(s): Jin-Feng DING, Jing-Xi LI, Cheng-Jun SUN, Chang-Fei HE, Feng-Hua JIANG, Feng-Lei GAO, Li ZHENGAbstractA pretreatment method was established for separating microplastics from digestive system of bivalve sample. Qualitative and quantitative analysis of microplastics was carried out by micro-Fourier transformed infrared (μ-FT-IR) spectroscope and Stereo microscope. The method was applied to analyze the microplastics in the digestive system in Chlamys farreri and Mytilus galloprovincialis. The results showed that the digest...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Preparation and in Vitro Anti-Laryngeal Cancer Evaluation of Protopanaxadiol-Loaded Hollow Gold Nanoparticles
Publication date: May 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 5Author(s): He-Jie LI, Zhe LIU, Xiao-Ran DENG, Jun LIN, Ping-An MA, Bo TENGAbstractProtopanaxadiol (PPD) has inhibitory effects on a variety of tumor cells, but the poor water solubility and low availability limit its clinical application. By using polyethylene glycol (PEG)-modified hollow gold nanoparticles (HAuNs-PEG) as the transport carriers, we prepared here the PPD-loaded HAuNs-PEG (HAuNs-PEG-PPD). And then we conducted a number of experiments to investigate the in vitro anti-laryngeal cancer effect (Hep-2 cell as a model) of ...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Ultra-small CuS Nanoparticles as Peroxidase Mimetics for Sensitive and Colorimetric Detection of Uric Acid in Human Serum
Publication date: May 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 5Author(s): Xue WANG, Cheng-Ling TANG, Jia-Jun LIU, Hong-Zhi ZHANG, Jian WANGAbstractAs the main final products of the purine metabolism in human body, uric acid (UA) usually presents in serum and urine. Thus, the level of UA in biology is closely related to human health. In this work, the ultra-small CuS nanoparticles (NPs) were demonstrated to possess intrinsic peroxidase-like activity towards 3,3',5,5'-tetramethylbenzidine (TMB) substrate in the presence of H2O2, which yielded the blue oxidized TMB (oxTMB) with strong absorption ...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Stimuli-Triggered Strand Displacement-Based Multifunctional Gene Detection Platform Controlled By A Multi-Input DNA Logic Gate
Publication date: May 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 5Author(s): Shuang WU, Pai PENG, Hui-Hui WANG, Tao LIAbstractHighly sensitive detection of various cancer related genes is of great significance in a number of biomedical applications. Here we describe a logic-controlled multifunctional platform that is capable of detecting two kinds of gene sequences with a 2-aminopurine (2-AP) as a quencher-free fluorescent probe, the fluorescence of which dramatically increases when it loops out the DNA helices. This detection platform is assembled from the split ATP aptamer, G-quadruplex, and th...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Progresses of Single Molecular Fluorescence Resonance Energy Transfer in Studying Biomacromolecule Dynamic Process
Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Le-Le SUN, Ying-Ying SU, Yan-Jing GAO, Wei Li, Hui LYU, Bin LI, Di LIAbstractBiomacromolecules participate in various kinds of vital processes. Observing and analyzing their structural dynamic and the dynamic processes of intermolecular interaction at molecular level are important for understanding the action mechanism. Since its advent, single molecular fluorescence resonance energy transfer (SM-FRET) has demonstrated its great potential in studying conformational change and interaction process of biomacromolecules, an...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Applications of Nanopore Sensing in Detection of Toxic Molecules
Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Shuo ZHOU, Peng TANG, Yun-Jiao WANG, Liang WANG, De-Qiang WANGAbstractAs a novel analytical method, nanopore sensing is widely applied in many fields such as nucleic acids sequencing, protein/peptides analysis, detection of metal ions and biomacromolecules including virus, bacteria, etc. With the growing public concerns on dietary safety and public security, there has been a greater demand on the detection of toxic molecules. With high sensitivity and selectivity, nanopore sensing is considered as a more powerful assay,...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Real-time Event Recognition and Analysis System for Nanopore Study
Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Hui-Feng WANG, Fei HUANG, Zhen GU, Zheng-Li HU, Yi-Lun YING, Bing-Yong YAN, Yi-Tao LONGAbstractTo achieve fast and accurate analysis of weak current signal of nanopore-based single molecule detection, an online data process based on adaptive threshold algorithm with data buffering technique and finite impulse response filtering was designed. A software system based on the data process was developed for online recognition and analysis of nanopore events during nanopore experiment. To testify the performance of the algori...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Research Methods for Structural Analysis of Lactic Acid Bacteria Induced Exopolysaccharides
In this report, we summarized the current progress of key methodologies involved in the structural analysis of LAB-generated EPSs, including their isolation, purification, primary structure and advances in structural research. A comprehensive discussion regarding the application of chemical analysis, instrument analysis and computer aided technology in the structure analysis of LAB-generated EPSs was provided. Further, the future development of the research on the structure of LAB-generated EPSs was also presented.Graphical abstractThe structural analysis of exopolysaccharides produced by lactobacillus is the basis for exp...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research