Real-time Event Recognition and Analysis System for Nanopore Study

Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Hui-Feng WANG, Fei HUANG, Zhen GU, Zheng-Li HU, Yi-Lun YING, Bing-Yong YAN, Yi-Tao LONGAbstractTo achieve fast and accurate analysis of weak current signal of nanopore-based single molecule detection, an online data process based on adaptive threshold algorithm with data buffering technique and finite impulse response filtering was designed. A software system based on the data process was developed for online recognition and analysis of nanopore events during nanopore experiment. To testify the performance of the algorithm and software system, ideal signals with different noise level (20–100 pA) were generated at bandwidth ranging from 3 kHz to 100 kHz. The result showed that this software system was stable at different bandwidths and sampling rates and could be used in analyzing the signals at high noise. The proposed software system was further applied to aerolysin nanopore experiment for detection of poly(dA)4 molecules. The results showed that the data process system could be applied in real nanopore recording experiment with high accuracy and speed.Graphical abstractTo achieve fast and accurate processing of nanopore experimental data, we designed a real-time adaptive threshold data processing algorithm with data buffering technique and finite impulse response filtering. The system has been designed based on the proposed data processing algorithm, which could achiev...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research