Chinese Journal of Analytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Quantitative Detection of Gene Methylated Level of Stool Samples Based on Invader Assay Coupled with Real-time Polymerase Chain Reaction and Its Application in Non-invasive Screening of Colorectal Cancer
Publication date: October 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 10Author(s): Lin-Lin LIU, Xie-Min QI, Bing-Jie ZOU, Qin-Xin SONG, Guo-Hua ZHOUAbstractMethylation of bone morphogenetic protein 3 (BMP3) in stool DNA is an effective biomarker for non-invasive screening of colorectal cancer. However, a highly sensitive and specific detection method is required. Here, a quantification method for BMP3 methylation was developed by combining real-time polymerase chain reaction (PCR) with invader assay using Beta-actin (ACTB) as a reference. Amplification efficiencies of BMP3 and ACTB were close to 1...
Source: Chinese Journal of Analytical Chemistry - October 17, 2018 Category: Chemistry Source Type: research

Screening of Clenbuterol Hydrochloride Aptamers Based on Capillary Electrophoresis
In this study, we performed the aptamer selection towards a small molecule target of clenbuterol hydrochloride (Clen) by CE-SELEX. In brief, Clen were first incubated with an 80 nt ssDNA library, and CZE-UV approach was used to separate complex and random ssDNA. The complex was then collected into a vial followed by PCR amplification. Through three round selections, the third library was selected to clone and ten sequences were finally obtained. The dissociation constant (Kd) of three potential candidates (Apt 4, Apt 7 and Apt 12) were determined by CE-LIF, and showed high affinities of 9.315 × 10−7 M, 1.040 × 10−6 M...
Source: Chinese Journal of Analytical Chemistry - October 17, 2018 Category: Chemistry Source Type: research

Iolite Based Bulk Normalization as 100% (m/m) Quantification Strategy for Reduction of Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry Transient Signal
In this study, the application of Iolite package was investigated for LA-ICP-MS elemental quantification, and a calculation code for the bulk normalization as 100% (m/m) strategy was compiled. We found that the spline interpolation approach was better than that of linear one for the correction of time-dependent instrument drift. BCR-2G as the quality material was used to assess the proposed code, and the results revealed that the code was practical and reliable. The analytical accuracy was influenced by the used calibration materials. TiO2, MgO, K2O and rare earth elements in BCR-2G were slightly off (5%–10%) when NIST S...
Source: Chinese Journal of Analytical Chemistry - October 17, 2018 Category: Chemistry Source Type: research

Rapid Determination of Heroin in Adulterated Commercial Beverage in Drug Transportation Cases
Publication date: October 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 10Author(s): Run-Fang XIE, Zhi-Qiang LU, Yu-Fang CHAI, Xi-Hao WANG, Hui-Fangjie LI, Jin-Yun GUAbstractTo avoid supervision when crossing borders or at the airport customs, smugglers increasingly use body packing to transport drugs. Now, more secretive transportation methods used for smaller quantities have brought difficulties and challenges to forensic toxicologists. To identify heroin in adulterated beverage, a quantification technique using gas chromatography-mass spectrometry (GC-MS) for beverage samples was developed, and h...
Source: Chinese Journal of Analytical Chemistry - October 17, 2018 Category: Chemistry Source Type: research

Influence of Temperature on Surface Morphology and Photoelectric Performance of CuAlO2 Thin Films
Publication date: October 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 10Author(s): Chen CHEN, Rui-Xin SHI, Chuan-Tao ZHENG, Yi-Ding WANGAbstractCuAlO2 thin films were prepared on sapphire substrate by sol-gel method. The influences of annealing temperature, heating rates and annealing time on composition, morphology and photoelectric performance of film were investigated. The results of X-ray power diffraction, scanning electron microscope, transmissivity, and hall parameter tests showed that the CuAlO2 phase was relatively pure when the annealing temperature was above 950 °C. The higher the temp...
Source: Chinese Journal of Analytical Chemistry - October 17, 2018 Category: Chemistry Source Type: research

Advances in Detection Methods of β-Amyloid Protein
Publication date: September 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 9Author(s): Meng JIANG, Xiao-Ying WANG, Xiao-Bing WANGAbstractAlzheimer's disease (AD) is a neurodegenerative disease with unknown etiology. β-amyloid protein (Aβ) is one of the specific biomarkers of AD, and many clinical studies suggest that abnormal levels of Aβ in blood, cerebrospinal fluid and brain tissue are closely related to the progression of AD. The analysis and evaluation of Aβ are important for early detection, tracking, prevention, and treatment of AD. In this paper, the present situations of the commonly use...
Source: Chinese Journal of Analytical Chemistry - September 5, 2018 Category: Chemistry Source Type: research

Research Advances in Ion Channel-based Electrochemical Sensing Techniques
Publication date: September 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 9Author(s): Xiao-Jing JIANG, Rong-Ning LIANG, Wei QINAbstractDuring the past few years, the electrochemical sensing techniques based on ion channels have attracted considerable attention. Nowadays, these techniques have been widely used in DNA sequencing, measurement of molecular interactions, and detection of inorganic ions and biological species. Hence, in this review, the research progresses of the ion channel-based electrochemical techniques including amperometry, conductometry and potentiometry in chemical and biological ...
Source: Chinese Journal of Analytical Chemistry - September 5, 2018 Category: Chemistry Source Type: research

Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells
Publication date: September 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 9Author(s): Yan-Hong SUN, Jia WEI, Zhen-Xin WANG, Xian-Ying MENGAbstractHerein, two kinds of ssDNA-GNPs (termed as PolyG1-GNPs and PolyG2-GNPs, respectively) were synthesized by modification of two different G-rich single-strand oligonucleotides (PolyG1 and PolyG2) onto 13-nm citrate-protected gold nanoparticles (GNPs) surface through formation of the sulfur-gold covalent bond, respectively. The as-prepared PolyG1-GNPs and PolyG2-GNPs had reasonable colloidal stability in complex dispersing matrixes. The effect of ssDNA sequen...
Source: Chinese Journal of Analytical Chemistry - September 5, 2018 Category: Chemistry Source Type: research

Study of Monolithic Integrated Micro Gas Chromatography Chip
Publication date: September 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 9Author(s): Bo-Wen TIAN, Fei FENG, Bin ZHAO, Fan LUO, Xue-Lei YANG, Hai-Mei ZHOU, Xin-Xin LIAbstractA monolithic micro gas chromatography (μGC) chip which integrated the micro separation column (μSC) and the micro thermal conductivity detector (μTCD) based on MEMS (Micro-electro-mechanical systems) technique was fabricated. Compared to the state of the art, the μSC with high depth-to-width ratio channels that was coated with mesoporous silica nanoparticles as stationary phase could effectively improve the column capacity a...
Source: Chinese Journal of Analytical Chemistry - September 5, 2018 Category: Chemistry Source Type: research

Preparation of Cyclodextrin Type Stationary Phase Based on Graphene Oxide and Its Application in Enantioseparation and Hydrophilic Interaction Chromatography
Publication date: September 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 9Author(s): Qiang LI, Yuan-Yuan LI, Nan ZHU, Zhu-Xian GAO, Tian-Jun LI, Tong ZHOU, Yu-Long MAAbstractGraphene oxide (GO) was covalently coupled to the surface of amino silica gel by amide bond. β-cyclodextrin (β-CD) was further chemically bonded with GO to prepare a novel chiral stationary phase. The resulting material was characterized by Fourier transform-infrared (FT-IR) spectra, scanning electron microscopy (SEM), transmission electron microscopy (TEM), elemental analysis and thermogravimetric analysis (TGA). The separat...
Source: Chinese Journal of Analytical Chemistry - September 5, 2018 Category: Chemistry Source Type: research

High-sensitivity Mercury Ion Detection System Using Unmodified Gold Nanorods
We report on a high-sensitivity Hg2+ detection method based on the localized surface plasmon resonance property of gold nanorods. The aspect ratio of gold nanorods gradually decreased to 1 and longitudinal absorption peak presented linear blue shift as Hg2+ concentration increases. (Source: Chinese Journal of Analytical Chemistry)
Source: Chinese Journal of Analytical Chemistry - September 5, 2018 Category: Chemistry Source Type: research

Progresses of Magnetic Relaxation Switch Sensor in Medical Diagnosis and Food Safety Analysis
Publication date: August 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 8Author(s): Gui-Ying GU, Xin WANG, Hua-Lan ZHOU, Bao-Lin LIUAbstractFast and accurate detection of complex samples in either medical diagnosis or food safety analysis are of great significance for the safeguarding of human health. Magnetic relaxation switches (MRS) technique based on superparamagnetic nanoparticles (SMP) is the combination of nano-biotechnology, nuclear magnetic resonance, chemistry and immunoassay. MRS assay has the characteristics of high sensitivity and specificity, as well as nondestructive and time-saving, a...
Source: Chinese Journal of Analytical Chemistry - July 27, 2018 Category: Chemistry Source Type: research

Dual-mode Detection of Dopamine Based on Enhanced Fluorescent and Colorimetric Signals of Fe3+-H2O2-o-Phenylenediamine System
Publication date: August 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 8Author(s): Chuan-Xia CHEN, Peng-Juan NI, Yuan-Yuan JIANG, Zhen-Lu ZHAO, Yi-Zhong LUAbstractA fluorescent and colorimetric dual-mode “light-on” assay for the detection of dopamine (DA) was developed based on Fe3+-H2O2-OPD system. In general, Fe3+ could catalyze the H2O2-mediated oxidation of colorless and nonfluorescent o-phenylenediamine (OPD), and the resultant 2,3-diaminophenazine (DAP) exhibits a visible yellow color and yellow fluorescence. However, the reaction rate is extremely slow. By comparison, the introduction of ...
Source: Chinese Journal of Analytical Chemistry - July 27, 2018 Category: Chemistry Source Type: research

Preparation of Poly(vinyl alcohol) Microspheres Based on Droplet Microfluidic Technology
Publication date: August 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 8Author(s): Xian-Wei HAN, Hong-Wu ZHANG, Hong-Yan LUO, Xiao-Lin ZHENG, Zhong YANG, Ning HU, Yan-Jian LIAO, Jun YANGAbstractA new method for preparing poly (vinyl alcohol) (PVA) microspheres was developed by using droplet microfluidic technology. In the microfluidic chip, a large number of uniform, monodispersed PVA droplets were prepared quickly and continuously by using droplet formation technology, and the droplet preparation speed reached 7 per second. The size of the PVA droplets could be controlled by changing the injection ...
Source: Chinese Journal of Analytical Chemistry - July 27, 2018 Category: Chemistry Source Type: research

Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Tylosin in Milk and Water Samples
Publication date: August 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 8Author(s): Chan-Yuan YAO, Jin-Yi YANG, Zhen-Lin XU, Hong WANG, Hong-Tao LEI, Yuan-Ming SUN, Yuan-Xin TIAN, Yu-Dong SHENAbstractA specific polyclonal antibody was prepared based on a new hapten with stable structure. Based on this, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for determination of tylosin residues in food and environmental samples. The experimental conditions were optimized as follows: the coating antigen and antibody were respectively diluted by 6000 times and 2000 times, an...
Source: Chinese Journal of Analytical Chemistry - July 27, 2018 Category: Chemistry Source Type: research