Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells

Publication date: September 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 9Author(s): Yan-Hong SUN, Jia WEI, Zhen-Xin WANG, Xian-Ying MENGAbstractHerein, two kinds of ssDNA-GNPs (termed as PolyG1-GNPs and PolyG2-GNPs, respectively) were synthesized by modification of two different G-rich single-strand oligonucleotides (PolyG1 and PolyG2) onto 13-nm citrate-protected gold nanoparticles (GNPs) surface through formation of the sulfur-gold covalent bond, respectively. The as-prepared PolyG1-GNPs and PolyG2-GNPs had reasonable colloidal stability in complex dispersing matrixes. The effect of ssDNA sequence on the interaction between ssDNA-GNPs and cells was systematically investigated by UV-visible absorption spectroscopy, cellular transmission electronic microscopy and inductively coupled plasma-mass spectrometry. The experimental results showed that both of the as-prepared PolyG1-GNPs and PolyG2-GNPs had low cytotoxicity and exhibited energy related endocytosis. In addition, the cellular internalization amount and dispersibility of ssDNA-GNPs were strongly affected by the sequences of immobilized ssDNAs. PolyG2 with G-quadruplex (G4) secondary structure could significantly increase the cellular internalization amount and intracellular stability of PolyG2-GNPs.Graphical abstractTwo ssDNA-GNPs (PolyG1-GNPs and PolyG2-GNPs) were synthesized by modification of two different of G-rich single strand oligonucleotides (PolyG1 and PolyG2) onto gold nanoparticle (G...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research