Chinese Journal of Analytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Development of a High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Lipophilic Toxins in Marine Shellfishes and Edible Safety Evaluation
In this study, several common lipophilic shellfish toxins (LSTs) in marine shellfishes were simultaneously detected by liquid chromatography-tandem mass spectrometry, and the safety risk of commercial marine shellfishes was evaluated based on the materiome of LSTs. Under the optimal conditions, the developed method displayed satisfactory recovery values (63.2%–88.8%), precision (relative standard deviations ≤ 14.5%), and sensitivity (limit of detection in the range of 0.54–2.69 ng g−1) for all analytes. Among the 105 commercially available shellfish samples, 42.86% of the samples had at least one kind of toxins. Th...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Protein Detection Through Single Molecule Nanopore
Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Yi LIU, Xu-Feng YAO, Hai-Yan WANGAbstractA single nanopore represents a versatile single-molecule probe that can be employed to reveal several important features of proteins, such as physical structure, backbone flexibility, mechanical stability, their folding state, binding affinity to other interacting ligands and enzymatic activity. In this review, we summarize the development and current research related to the field of protein detection by nanopore, as well as a few examples of the pioneer work on protein detection...
Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research

Stimuli-Triggered Strand Displacement-Based Multifunctional Gene Detection Platform Controlled By A Multi-Input DNA Logic Gate
Publication date: May 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 5 Author(s): Shuang WU, Pai PENG, Hui-Hui WANG, Tao LI Highly sensitive detection of various cancer related genes is of great significance in a number of biomedical applications. Here we describe a logic-controlled multifunctional platform that is capable of detecting two kinds of gene sequences with a 2-aminopurine (2-AP) as a quencher-free fluorescent probe, the fluorescence of which dramatically increases when it loops out the DNA helices. This detection platform is assembled from the split ATP aptamer, G-quadruplex, and th...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Ultra-small CuS Nanoparticles as Peroxidase Mimetics for Sensitive and Colorimetric Detection of Uric Acid in Human Serum
Publication date: May 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 5 Author(s): Xue WANG, Cheng-Ling TANG, Jia-Jun LIU, Hong-Zhi ZHANG, Jian WANG As the main final products of the purine metabolism in human body, uric acid (UA) usually presents in serum and urine. Thus, the level of UA in biology is closely related to human health. In this work, the ultra-small CuS nanoparticles (NPs) were demonstrated to possess intrinsic peroxidase-like activity towards 3,3',5,5'-tetramethylbenzidine (TMB) substrate in the presence of H2O2, which yielded the blue oxidized TMB (oxTMB) with strong absorption...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Preparation and in Vitro Anti-Laryngeal Cancer Evaluation of Protopanaxadiol-Loaded Hollow Gold Nanoparticles
Publication date: May 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 5 Author(s): He-Jie LI, Zhe LIU, Xiao-Ran DENG, Jun LIN, Ping-An MA, Bo TENG Protopanaxadiol (PPD) has inhibitory effects on a variety of tumor cells, but the poor water solubility and low availability limit its clinical application. By using polyethylene glycol (PEG)-modified hollow gold nanoparticles (HAuNs-PEG) as the transport carriers, we prepared here the PPD-loaded HAuNs-PEG (HAuNs-PEG-PPD). And then we conducted a number of experiments to investigate the in vitro anti-laryngeal cancer effect (Hep-2 cell as a model) o...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Separation and Identification of Microplastics in Digestive System of Bivalves
Publication date: May 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 5 Author(s): Jin-Feng DING, Jing-Xi LI, Cheng-Jun SUN, Chang-Fei HE, Feng-Hua JIANG, Feng-Lei GAO, Li ZHENG A pretreatment method was established for separating microplastics from digestive system of bivalve sample. Qualitative and quantitative analysis of microplastics was carried out by micro-Fourier transformed infrared (μ-FT-IR) spectroscope and Stereo microscope. The method was applied to analyze the microplastics in the digestive system in Chlamys farreri and Mytilus galloprovincialis. The results showed that the dig...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Development of Microchip Electrophoresis and Its Applications in Ion Detection
Publication date: May 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 5 Author(s): Ming-Peng YANG, Zhe HUANG, Yang XIE, Hui YOU Microchip electrophoresis is emerging as a highly promising method due to its portability and fast analysis with a minimum number of analytes. In this review, recent developments in microchip electrophoresis were described with discussions on the structures, materials, processing technologies, surface modification, experimental methods, and applications in ion detection. The existing issues and prospects of future studies were discussed as well. Graphical abstract (Sour...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Protein Detection Through Single Molecule Nanopore
Publication date: June 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 6 Author(s): Yi LIU, Xu-Feng YAO, Hai-Yan WANG Abstract A single nanopore represents a versatile single-molecule probe that can be employed to reveal several important features of proteins, such as physical structure, backbone flexibility, mechanical stability, their folding state, binding affinity to other interacting ligands and enzymatic activity. In this review, we summarize the development and current research related to the field of protein detection by nanopore, as well as a few examples of the pioneer work on protein d...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Development of a High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Lipophilic Toxins in Marine Shellfishes and Edible Safety Evaluation
In this study, several common lipophilic shellfish toxins (LSTs) in marine shellfishes were simultaneously detected by liquid chromatography-tandem mass spectrometry, and the safety risk of commercial marine shellfishes was evaluated based on the materiome of LSTs. Under the optimal conditions, the developed method displayed satisfactory recovery values (63.2%–88.8%), precision (relative standard deviations ≤ 14.5%), and sensitivity (limit of detection in the range of 0.54–2.69 ng g−1) for all analytes. Among the 105 commercially available shellfish samples, 42.86% of the samples had at least one kind of toxins. Th...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Research Methods for Structural Analysis of Lactic Acid Bacteria Induced Exopolysaccharides
In this report, we summarized the current progress of key methodologies involved in the structural analysis of LAB-generated EPSs, including their isolation, purification, primary structure and advances in structural research. A comprehensive discussion regarding the application of chemical analysis, instrument analysis and computer aided technology in the structure analysis of LAB-generated EPSs was provided. Further, the future development of the research on the structure of LAB-generated EPSs was also presented. Graphical abstract (Source: Chinese Journal of Analytical Chemistry)
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Real-time Event Recognition and Analysis System for Nanopore Study
Publication date: June 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 6 Author(s): Hui-Feng WANG, Fei HUANG, Zhen GU, Zheng-Li HU, Yi-Lun YING, Bing-Yong YAN, Yi-Tao LONG Abstract To achieve fast and accurate analysis of weak current signal of nanopore-based single molecule detection, an online data process based on adaptive threshold algorithm with data buffering technique and finite impulse response filtering was designed. A software system based on the data process was developed for online recognition and analysis of nanopore events during nanopore experiment. To testify the performance o...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Applications of Nanopore Sensing in Detection of Toxic Molecules
Publication date: June 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 6 Author(s): Shuo ZHOU, Peng TANG, Yun-Jiao WANG, Liang WANG, De-Qiang WANG Abstract As a novel analytical method, nanopore sensing is widely applied in many fields such as nucleic acids sequencing, protein/peptides analysis, detection of metal ions and biomacromolecules including virus, bacteria, etc. With the growing public concerns on dietary safety and public security, there has been a greater demand on the detection of toxic molecules. With high sensitivity and selectivity, nanopore sensing is considered as a more power...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Progresses of Single Molecular Fluorescence Resonance Energy Transfer in Studying Biomacromolecule Dynamic Process
Publication date: June 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 6 Author(s): Le-Le SUN, Ying-Ying SU, Yan-Jing GAO, Wei Li, Hui LYU, Bin LI, Di LI Abstract Biomacromolecules participate in various kinds of vital processes. Observing and analyzing their structural dynamic and the dynamic processes of intermolecular interaction at molecular level are important for understanding the action mechanism. Since its advent, single molecular fluorescence resonance energy transfer (SM-FRET) has demonstrated its great potential in studying conformational change and interaction process of biomacrom...
Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research

Determination of Benzotriazole and Its Derivatives in Aqueous Sample with Air-assisted Liquid-Liquid Microextraction Followed by High-performance Liquid Chromatography
Publication date: April 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 4 Author(s): Jing LU, Meng-Meng WANG, Qiang WANG, Hai-Pu LI, Zhao-Guang YANG Benzotriazole and its derivatives are persistent contaminants and ubiquitous in aquatic environments. Here, a simple, reliable method based on air-assisted liquid-liquid microextraction coupled with high-performance liquid chromatography with a UV detector for determining 1H-benzotriazole, 5-methyl-1H-benzotriazole and 5-chloro-1H-benzotriazole in aqueous samples was developed. In this procedure, the analytes quickly entered into the extraction sol...
Source: Chinese Journal of Analytical Chemistry - April 10, 2018 Category: Chemistry Source Type: research

Components Analysis of Biochar Based on Near Infrared Spectroscopy Technology
This study aims to establish a rapid quantitative analysis method for biochar based on near infrared spectroscopy (NIRS) technology. Near infrared spectra of 163 samples in the 10000–3800 cm–1 (1000–2632 nm) range were collected, and the contents of fixed carbon (FC), volatile matter (VM) and ash of samples were also analyzed. A partial least square (PLS) model for FC, VM and Ash was established after the model spectral ranges were optimized, the optimal factors were determined, and the raw spectra were pretreated by multiple scatter correction and second derivative (MSC + SD) method. Finally, the prediction performa...
Source: Chinese Journal of Analytical Chemistry - April 10, 2018 Category: Chemistry Source Type: research