Progresses of Single Molecular Fluorescence Resonance Energy Transfer in Studying Biomacromolecule Dynamic Process

Publication date: June 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 6 Author(s): Le-Le SUN, Ying-Ying SU, Yan-Jing GAO, Wei Li, Hui LYU, Bin LI, Di LI Abstract Biomacromolecules participate in various kinds of vital processes. Observing and analyzing their structural dynamic and the dynamic processes of intermolecular interaction at molecular level are important for understanding the action mechanism. Since its advent, single molecular fluorescence resonance energy transfer (SM-FRET) has demonstrated its great potential in studying conformational change and interaction process of biomacromolecules, and a series of new mechanisms have been revealed. This review summarized recent progresses of SM-FRET in studying protein structural dynamic, nucleic acid structural dynamic, protein-protein and protein-nucleic acid interactions. Graphical abstract

Source: Chinese Journal of Analytical Chemistry - June 12, 2018 Category: Chemistry Source Type: research