Determination of Benzotriazole and Its Derivatives in Aqueous Sample with Air-assisted Liquid-Liquid Microextraction Followed by High-performance Liquid Chromatography

Publication date: April 2018 Source:Chinese Journal of Analytical Chemistry, Volume 46, Issue 4 Author(s): Jing LU, Meng-Meng WANG, Qiang WANG, Hai-Pu LI, Zhao-Guang YANG Benzotriazole and its derivatives are persistent contaminants and ubiquitous in aquatic environments. Here, a simple, reliable method based on air-assisted liquid-liquid microextraction coupled with high-performance liquid chromatography with a UV detector for determining 1H-benzotriazole, 5-methyl-1H-benzotriazole and 5-chloro-1H-benzotriazole in aqueous samples was developed. In this procedure, the analytes quickly entered into the extraction solvent via the bolus flow formed during the movement of the plunger without disperser solvent. 1-Hexanol was used as the extractant, thus avoiding chlorinated solvents, and a narrow-neck tube with round bottom was self-made to facilitate collection of the low-density extract phase and centrifugation at high speed. Those main parameters, including sample volume, type and volume of extraction solvent, number of suction-injection cycle, salt addition, and the pH were optimized. Under the optimized conditions, the pretreatment could be completed within two minutes with a good linearity (r > 0.9996). Satisfactory intra-day (3.0%–4.3%, n = 5) and inter-day (5.9%–7.8%, n = 5) precisions were observed. Further, the method was applied in analysis of real samples including tap water, lake water, river water, and municipal waste water with good recoveries (73%...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research