Protein Detection Through Single Molecule Nanopore

Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Yi LIU, Xu-Feng YAO, Hai-Yan WANGAbstractA single nanopore represents a versatile single-molecule probe that can be employed to reveal several important features of proteins, such as physical structure, backbone flexibility, mechanical stability, their folding state, binding affinity to other interacting ligands and enzymatic activity. In this review, we summarize the development and current research related to the field of protein detection by nanopore, as well as a few examples of the pioneer work on protein detection. We first discuss the principle of electrical detection with nanopores and how this technique provides information from current traces. Then the development from peptide detection with biological nanopore to protein detection through solid-state nanopore is described. Finally, we prospect the measurement of protein shape and construction using nanopore technology for the applications in life research area.Graphical abstractNanopore-based single molecule detection method provides analytical capabilities through a nanoscale pore with broad bio-applications, as well as represent single-molecule probes for examining the flexibility and folding state of single proteins. Hence, we summarized nanopore provide new generation research tools in protein for complex recognition and expected further developments on designing pores.
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research