Preparation and in Vitro Anti-Laryngeal Cancer Evaluation of Protopanaxadiol-Loaded Hollow Gold Nanoparticles

Publication date: May 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 5Author(s): He-Jie LI, Zhe LIU, Xiao-Ran DENG, Jun LIN, Ping-An MA, Bo TENGAbstractProtopanaxadiol (PPD) has inhibitory effects on a variety of tumor cells, but the poor water solubility and low availability limit its clinical application. By using polyethylene glycol (PEG)-modified hollow gold nanoparticles (HAuNs-PEG) as the transport carriers, we prepared here the PPD-loaded HAuNs-PEG (HAuNs-PEG-PPD). And then we conducted a number of experiments to investigate the in vitro anti-laryngeal cancer effect (Hep-2 cell as a model) of HAuNs-PEG-PPD, such as using high performance liquid chromatography (HPLC) to detect the sustained release effect, MTT assay to detect the inhibitory effect on the proliferation of Hep-2 cells, and flow cytometry to determine the apoptosis of Hep-2 cells. The results showed that HAuNs-PEG-PPD had an obvious sustained release effect. Compared with those in the control group, HAuNs-PEG group and PPD group, the survival rate of Hep-2 cells decreased and the apoptosis rate increased significantly (p < 0.01) in the HAuNs-PEG-PPD group. The HAuNs-PEG- PPD could significantly enhance the anti-laryngeal cancer effect of PPD and promote the apoptosis of tumor cells. Thus, the HAuNs is a promising drug delivery system of PPD in clinical treatment of laryngeal cancer.Graphical abstractAs a new type of nanoparticle drug carrier, hollow gold nanoparticle has the adva...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research