Progresses of Single Molecular Fluorescence Resonance Energy Transfer in Studying Biomacromolecule Dynamic Process

Publication date: June 2018Source: Chinese Journal of Analytical Chemistry, Volume 46, Issue 6Author(s): Le-Le SUN, Ying-Ying SU, Yan-Jing GAO, Wei Li, Hui LYU, Bin LI, Di LIAbstractBiomacromolecules participate in various kinds of vital processes. Observing and analyzing their structural dynamic and the dynamic processes of intermolecular interaction at molecular level are important for understanding the action mechanism. Since its advent, single molecular fluorescence resonance energy transfer (SM-FRET) has demonstrated its great potential in studying conformational change and interaction process of biomacromolecules, and a series of new mechanisms have been revealed. This review summarized recent progresses of SM-FRET in studying protein structural dynamic, nucleic acid structural dynamic, protein-protein and protein-nucleic acid interactions.Graphical abstractSingle molecule fluorescence resonance energy transfer can obtain the fluorescence intensity trajectories of fluorescent donors and receptors on a single biomolecule, and the corresponding Eapp trajectories through apparent fluorescence resonance energy efficiency (Eapp) calculation, thereby real-time monitoring the conformational dynamics of functional biomolecule, which has great potential in studying the action mechanisms of biomolecular.

Source: Chinese Journal of Analytical Chemistry - July 5, 2018 Category: Chemistry Source Type: research