Plasmodium falciparum UvrD activities are downregulated by DNA-interacting compounds and its dsRNA inhibits malaria parasite growth
Conclusions:
As PfUvrD is absent in human hence on the basis of this study we propose PfUvrD as suitable drug target to control malaria. Some of the PfUvrD inhibitors identified in the present study can be utilized to further design novel and specific inhibitor molecules. (Source: BMC Biochemistry)
Source: BMC Biochemistry - April 3, 2014 Category: Biochemistry Authors: Mohammed TariqueFarha TabassumMoaz AhmadRenu Tuteja Source Type: research
Expression, purification and characterization of soluble red rooster laforin as a fusion protein in Escherichia coli
Conclusions:
Gg-laforin is more soluble and stable than human laforin in vitro, and possesses similar activity as a glucan phosphatase. Therefore, it can be used to model human laforin in structure-function studies. We have established a protocol for purifying recombinant Gg-laforin in sufficient quantity for crystallographic and other biophysical analyses, in order to better understand the function of laforin and define the molecular mechanisms of Lafora disease. (Source: BMC Biochemistry)
Source: BMC Biochemistry - April 2, 2014 Category: Biochemistry Authors: M BrewerSatrio HusodoVikas DukhandeMary JohnsonMatthew Gentry Source Type: research
Functional expression and activity of the recombinant antifungal defensin PvD1r from Phaseolus vulgaris L. (common bean) seeds
Conclusion:
Our results indicate that PvD1r retains full biological activity after recombinant production, enterokinase cleavage and purification. Additionally, our results from the antimicrobial assay, the microscopic analysis and the PvD1r-FITC labeling assays corroborate each other and lead us to suggest that the target of PvD1 in C. albicans cells is the sphingolipid glucosylceramide. (Source: BMC Biochemistry)
Source: BMC Biochemistry - April 1, 2014 Category: Biochemistry Authors: Érica de O MelloIzabela dos SantosAndré de O CarvalhoLuísa de SouzaGonçalo de Souza-FilhoViviane do NascimentoOlga MachadoUmberto ZottichValdirene Gomes Source Type: research
Determination of carbohydrate-deficient transferrin in a Han Chinese population
Conclusions:
These results demonstrate that CDT is a useful marker to detect heavy daily alcohol consumption. We proposed and evaluated the first CDT cut-off for the Han Chinese population, and we showed that the CDT capillary zone electrophoresis system is a reliable analytic method. (Source: BMC Biochemistry)
Source: BMC Biochemistry - February 27, 2014 Category: Biochemistry Authors: Binbin SongJing ZhuJiong WuChunyan ZhangBeili WangBaishen PanWei Guo Source Type: research
Mammalian target of Rapamycin inhibition and mycobacterial survival are uncoupled in murine macrophages
Conclusions:
This data, coupled with previous work from our laboratory, further indicates that autophagy induction by mTOR inhibition is an artificial means to increase mycobacterial killing and masks more relevant endogenous autophagic biochemistry that needs to be understood. (Source: BMC Biochemistry)
Source: BMC Biochemistry - February 14, 2014 Category: Biochemistry Authors: Alfred ZulloKristen Jurcic SmithSunhee Lee Source Type: research
Multiple autophosphorylations significantly enhance the endoribonuclease activity of human inositol requiring enzyme 1alpha
Conclusions:
Taken together these experiments support the hypothesis that phosphorylation is important in modulating IRE1alpha RNase activity which is achieved by increasing the propensity of IRE1alpha to dimerise. This work supports the development of IRE1alpha kinase inhibitors for use in the treatment of secretory cancers. (Source: BMC Biochemistry)
Source: BMC Biochemistry - February 13, 2014 Category: Biochemistry Authors: Daniel ItzhakMichael BrightPeter McAndrewAmin MirzaYvette NewbattJade StroverMarcella WidyaAndrew ThompsonGareth MorganIan CollinsFaith Davies Source Type: research
Differential roles of tryptophan residues in conformational stability of Porphyromonas gingivalis HmuY hemophore
Conclusions:
Here we demonstrate differential roles of tryptophan residues in the protein conformational stability. We also propose different conformations of apo- and holoHmuY caused by tertiary changes which allow heme binding to the protein. (Source: BMC Biochemistry)
Source: BMC Biochemistry - February 10, 2014 Category: Biochemistry Authors: Marcin BieleckiHalina WójtowiczTeresa Olczak Source Type: research
Biarsenical ligands bind to endogenous G-protein ¿-subunits and enable allosteric sensing of nucleotide binding
Conclusions:
Biarsenical ligands interact allosterically with endogenous G-protein alpha-subunits in a nucleotide-sensitive manner, so the presence or absence of guanine nucleotides has an effect on the fluorescence anisotropy, intensity and lifetime of F2FlAsH-G-protein complexes. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 17, 2013 Category: Biochemistry Authors: Lauri TõntsonSergei KopanchukAgo Rinken Source Type: research
Biarsenical ligands bind to endogenous G-protein alpha-subunits and enable allosteric sensing of nucleotide binding
Conclusions:
Biarsenical ligands interact allosterically with endogenous G-protein alpha-subunits in a nucleotide-sensitive manner, so the presence or absence of guanine nucleotides has an effect on the fluorescence anisotropy, intensity and lifetime of F2FlAsH-G-protein complexes. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 17, 2013 Category: Biochemistry Authors: Lauri TõntsonSergei KopanchukAgo Rinken Source Type: research
Soybean peroxidase-mediated degradation of an azo dye– a detailed mechanistic study
Conclusions:
Detailed analysis of metabolites, based on LC/MS results, showed that the enzyme-based degradation of the CP6R dye proceeded in two different reaction pathways- via symmetric azo bond cleavage as well as asymmetric azo bond breakage in the dye molecule. In addition, various critical transformative and oxidative steps such as deamination, desulfonation, keto-oxidation are explained on an electronic level. Furthermore, LC/MS/MS analyses confirmed that the end products in both pathways were small chain aliphatic carboxylic acids. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 5, 2013 Category: Biochemistry Authors: Liaquat AliRowdha AlgaithiHosam HabibUsama SoukaMuhammad RaufS Ashraf Source Type: research
Soybean peroxidase-mediated degradation of an azo dye¿ a detailed mechanistic study
Conclusions:
Detailed analysis of metabolites, based on LC/MS results, showed that the enzyme-based degradation of the CP6R dye proceeded in two different reaction pathways- via symmetric azo bond cleavage as well as asymmetric azo bond breakage in the dye molecule. In addition, various critical transformative and oxidative steps such as deamination, desulfonation, keto-oxidation are explained on an electronic level. Furthermore, LC/MS/MS analyses confirmed that the end products in both pathways were small chain aliphatic carboxylic acids. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 5, 2013 Category: Biochemistry Authors: Liaquat AliRowdha AlgaithiHosam HabibUsama SoukaMuhammad RaufS Ashraf Source Type: research
Soybean peroxidase-mediated degradation of an azo dye-- a detailed mechanistic study
Conclusions:
Detailed analysis of metabolites, based on LC/MS results, showed that the enzyme-based degradation of the CP6R dye proceeded in two different reaction pathways- via symmetric azo bond cleavage as well as asymmetric azo bond breakage in the dye molecule. In addition, various critical transformative and oxidative steps such as deamination, desulfonation, keto-oxidation are explained on an electronic level. Furthermore, LC/MS/MS analyses confirmed that the end products in both pathways were small chain aliphatic carboxylic acids. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 5, 2013 Category: Biochemistry Authors: Liaquat AliRowdha AlgaithiHosam HabibUsama SoukaMuhammad RaufS Ashraf Source Type: research
Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells
Conclusions:
The method described here represents a validated, simple and rapid bio-molecular assay to evaluate soluble HTT levels in blood cells as useful tool in disease and pharmacodynamic marker identification for observational and clinical trials. (Source: BMC Biochemistry)
Source: BMC Biochemistry - November 25, 2013 Category: Biochemistry Authors: Luisa MassaiLara PetriccaLetizia MagnoniLuca RovetiniSalman HaiderRalph AndreSarah TabriziSigurd SüssmuthBernhard LandwehrmeyerAndrea CaricasoleGiuseppe PollioSimonetta Bernocco Source Type: research
AGG/CCT interruptions affect nucleosome formation and positioning of healthy-length CGG/CCG triplet repeats
Conclusions:
This work defines the ability of AGG/CCT interruptions to modulate the behavior of the repeat tract in the packaged gene and contributes to our understanding of the role that AGG/CCT interruptions play in suppressing expansion and maintaining the correct functional organization of the FMR1 gene, highlighting a protective role played by the interruptions in genomic packaging. (Source: BMC Biochemistry)
Source: BMC Biochemistry - November 22, 2013 Category: Biochemistry Authors: Catherine VolleSarah Delaney Source Type: research
Characterization of a chemostable serine alkaline protease from Periplaneta americana
Conclusion:
Several important properties of a serine protease from P. Americana were revealed. Moreover, insects can serve as excellent and alternative source of industrially important proteases with unique properties, which can be utilized as additives in detergents, stain removers and other bio-formulations. Properties of the P. americana protease accounted in the present investigation can be exploited further in various industrial processes. As an industrial prospective, identification of enzymes with varying essential properties from different insect species might be good approach and bioresource. (Source: BMC Biochemistry)
Source: BMC Biochemistry - November 14, 2013 Category: Biochemistry Authors: Prashant SanatanPurushottam LomateAshok GiriVandana Hivrale Source Type: research
