Biarsenical ligands bind to endogenous G-protein alpha-subunits and enable allosteric sensing of nucleotide binding

Conclusions:
Biarsenical ligands interact allosterically with endogenous G-protein alpha-subunits in a nucleotide-sensitive manner, so the presence or absence of guanine nucleotides has an effect on the fluorescence anisotropy, intensity and lifetime of F2FlAsH-G-protein complexes.

Source: BMC Biochemistry - December 17, 2013 Category: Biochemistry Authors: Lauri TõntsonSergei KopanchukAgo Rinken Source Type: research