Soybean peroxidase-mediated degradation of an azo dye¿ a detailed mechanistic study

Conclusions:
Detailed analysis of metabolites, based on LC/MS results, showed that the enzyme-based degradation of the CP6R dye proceeded in two different reaction pathways- via symmetric azo bond cleavage as well as asymmetric azo bond breakage in the dye molecule. In addition, various critical transformative and oxidative steps such as deamination, desulfonation, keto-oxidation are explained on an electronic level. Furthermore, LC/MS/MS analyses confirmed that the end products in both pathways were small chain aliphatic carboxylic acids.

Source: BMC Biochemistry - December 5, 2013 Category: Biochemistry Authors: Liaquat AliRowdha AlgaithiHosam HabibUsama SoukaMuhammad RaufS Ashraf Source Type: research