Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays
The reduction of tetrazolium salts by NAD(P)H to formazan product has been widely used to determine the metabolic activity of cells, and as an indicator of cell viability. However, the application of a WST-8 b... (Source: BMC Biochemistry)
Source: BMC Biochemistry - April 8, 2019 Category: Biochemistry Authors: Kamonwan Chamchoy, Danaya Pakotiprapha, Pornpan Pumirat, Ubolsree Leartsakulpanich and Usa Boonyuen Tags: Methodology article Source Type: research
Association of TM6SF2 rs58542926 gene polymorphism with the risk of non-alcoholic fatty liver disease and colorectal adenoma in Chinese Han population
Genetic factors affect the risk of non-alcoholic fatty liver disease (NAFLD) and colorectal adenoma (CRA) importantly. Transmembrane protein 6 superfamily member 2 (TM6SF2) rs58542926 is a significant genetic ... (Source: BMC Biochemistry)
Source: BMC Biochemistry - February 6, 2019 Category: Biochemistry Authors: Yuan Li, Shousheng Liu, Yuqiang Gao, Huan Ma, Shuhui Zhan, Yan Yang, Yongning Xin and Shiying Xuan Tags: Research article Source Type: research
The active role of the transcription factor Sp1 in NFATc2-mediated gene regulation in pancreatic cancer
Adenocarcinoma of the pancreas is one of the most aggressive tumor diseases affecting the human body. The oncogenic potential of pancreatic cancer is mainly characterized by extremely rapid growth triggered by... (Source: BMC Biochemistry)
Source: BMC Biochemistry - January 29, 2019 Category: Biochemistry Authors: Manuela Malsy, Bernhard Graf and Katrin Almstedt Tags: Research article Source Type: research
Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteins
Many bacteria and certain eukaryotes utilize multi-step His-to-Asp phosphorelays for adaptive responses to their extracellular environments. Histidine phosphotransfer (HPt) proteins function as key components ... (Source: BMC Biochemistry)
Source: BMC Biochemistry - January 21, 2019 Category: Biochemistry Authors: Emily N. Kennedy, Skyler D. Hebdon, Smita K. Menon, Clay A. Foster, Daniel M. Copeland, Qingping Xu, Fabiola Janiak-Spens and Ann H. West Tags: Research article Source Type: research
Up-regulation of DcR3 in microbial toxins-stimulated HUVECs involves NF- κB signalling
Sepsis is a severe condition characterised by the body ’s systemic inflammatory response to infection. The specific sepsis-related biomarkers should be used in clinical diagnosis, therapeutic response monitorin... (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 27, 2018 Category: Biochemistry Authors: Yanqiang Hou, Dongyu Liang, Yang Liu, Hongwei Chen and Xiaoli Lou Tags: Research article Source Type: research
Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
Studying proteins and enzymes involved in important biological processes in the Aedes aegypti mosquito is limited by the quantity that can be directly isolated from the mosquito. Adding to this difficulty, digest... (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 18, 2018 Category: Biochemistry Authors: James T. Nguyen, Jonathan Fong, Daniel Fong, Timothy Fong, Rachael M. Lucero, Jamie M. Gallimore, Olive E. Burata, Kamille Parungao and Alberto A. Rasc ón Jr Tags: Methodology article Source Type: research
Identification of the major diacylglycerol acyltransferase mRNA in mouse adipocytes and macrophages
Triacylglycerols (TAGs) are the major form of energy storage in eukaryotes. Diacylglycerol acyltransferases (DGATs) catalyze the final and rate-limiting step of TAG biosynthesis. Mammalian DGATs are classified... (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 14, 2018 Category: Biochemistry Authors: Heping Cao Tags: Research article Source Type: research
Purification and characterization of cysteine protease from miswak Salvadora persica
In this study, protease was ... (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 3, 2018 Category: Biochemistry Authors: Wesam H. Abdulaal Tags: Research article Source Type: research
Effect of IAPP on the proteome of cultured Rin-5F cells
Islet amyloid polypeptide (IAPP) or amylin deposits can be found in the islets of type 2 diabetes patients. The peptide is suggested to be involved in the etiology of the disease through formation of amyloid d... (Source: BMC Biochemistry)
Source: BMC Biochemistry - November 12, 2018 Category: Biochemistry Authors: Samaneh Miraee-Nedjad, Paul F. G. Sims, Jean-Marc Schwartz and Andrew J. Doig Tags: Research article Source Type: research
Structure and function of a lignostilbene- α,β-dioxygenase orthologue from Pseudomonas brassicacearum
Stilbene cleaving oxygenases (SCOs), also known as lignostilbene- α,β-dioxygenases (LSDs) mediate the oxidative cleavage of the olefinic double bonds of lignin-derived intermediate phenolic stilbenes, yielding ... (Source: BMC Biochemistry)
Source: BMC Biochemistry - August 16, 2018 Category: Biochemistry Authors: Peter C. Loewen, Jacek Switala, James P. Wells, Fang Huang, Anthony T. Zara, John S. Allingham and Michele C. Loewen Tags: Research article Source Type: research
Simple spectrophotometric assay for measuring catalase activity in biological tissues
The details of a precise, accurate, and sensitive spectrophotometric method for measuring catalase activity are presented here. The assay was established for biological samples and depends on the rapid formati... (Source: BMC Biochemistry)
Source: BMC Biochemistry - August 3, 2018 Category: Biochemistry Authors: Mahmoud Hussein Hadwan Tags: Methodology article Source Type: research
Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma
Endonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosi... (Source: BMC Biochemistry)
Source: BMC Biochemistry - July 31, 2018 Category: Biochemistry Authors: Renquan Lu, Yingchao Wang, Xiaofeng Xu, Suhong Xie, Yanchun Wang, Ailing Zhong, Hui Zheng, Yiwen Yu, Xiang Gao and Lin Guo Tags: Research article Source Type: research
Isolation of quercetin and mandelic acid from Aesculus indica fruit and their biological activities
In this study Aesculus indica fruit was subjected to isolation of phytochemicals. Two antioxidants quercetin and Mandelic acid were isolated in pure state. The free radical scavenging and acetyl choline esterase ... (Source: BMC Biochemistry)
Source: BMC Biochemistry - June 26, 2018 Category: Biochemistry Authors: Muhammad Zahoor, Sadaf Shafiq, Habib Ullah, Abdul Sadiq and Farhat Ullah Tags: Research article Source Type: research
Purification and characterization of α-amylase from Trichoderma pseudokoningii
In this study the α-amylase was purified ... (Source: BMC Biochemistry)
Source: BMC Biochemistry - June 14, 2018 Category: Biochemistry Authors: Wesam H. Abdulaal Tags: Research article Source Type: research
Identification and characterization of smallest pore-forming protein in the cell wall of pathogenic Corynebacterium urealyticum DSM 7109
Corynebacterium urealyticum, a pathogenic, multidrug resistant member of the mycolata, is known as causative agent of urinary tract infections although it is a bacterium of the skin flora. This pathogenic bacteri... (Source: BMC Biochemistry)
Source: BMC Biochemistry - May 9, 2018 Category: Biochemistry Authors: Narges Abdali, Farhan Younas, Samaneh Mafakheri, Karunakar R. Pothula, Ulrich Kleinekath öfer, Andreas Tauch and Roland Benz Tags: Research article Source Type: research
