Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins
Conclusion:
Taken together, this is the first evidence of the generation and characterization of an active, full-length, mutant DUSP5 protein which will facilitate future structure-function and drug development-based studies. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 18, 2014 Category: Biochemistry Authors: Jaladhi NayakAdam GastonguayMarat TalipovPadmanabhan VakeelElise SpanKelsey KalousRaman KuttyDavin JensenPhani PokkuluriDaniel SemRajendra RathoreRamani Ramchandran Source Type: research
The promoter of cell growth- and RNA protection-associated SND1 gene is activated by endoplasmic reticulum stress in human hepatoma cells
Conclusion:
Promoter activity of the cell growth- and RNA-protection associated SND1 gene is up-regulated by ER stress in human hepatoma cells. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 11, 2014 Category: Biochemistry Authors: Sandra ArmengolEnara ArretxeLeire EnzunzaSarai MulaBegoña OchoaYolanda ChicoMaría Martínez Source Type: research
ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates
Conclusions:
We discovered an ADP-ribosylargininyl activity of cholix toxin catalytic fragment from our studies in auto-ADP-ribosylation, which is mediated through diffusible intermediates. The lifetime of the hypothetical intermediate exceeds recorded and predicted lifetimes for the cognate oxocarbenium ion. Therefore, a diffusible strained form of NAD+ intermediate was proposed to react with arginine residues in a proximity dependent manner. (Source: BMC Biochemistry)
Source: BMC Biochemistry - December 11, 2014 Category: Biochemistry Authors: Vicky SungChia-Lun Tsai Source Type: research
Phosphorylation in intrinsically disordered regions regulates the activity of Neurogenin2
Conclusions:
Phosphorylation of Ngn2 occurs in both the N- and C-terminal regions, either side of the conserved basic Helix-Loop-Helix domain. While these phosphorylation events do not change the intrinsic stability of Ngn2, phosphorylation on multiple sites acts to limit its ability to drive neuronal differentiation in vivo. Phosphorylated regions of Ngn2 are predicted to be intrinsically disordered and cdk-dependent phosphorylation of these intrinsically disordered regions contributes to Ngn2 regulation. (Source: BMC Biochemistry)
Source: BMC Biochemistry - November 6, 2014 Category: Biochemistry Authors: Gary McDowellChristopher HindleyGuy LippensIsabelle LandrieuAnna Philpott Source Type: research
Alleviation effect of arbutin on oxidative stress generated through tyrosinase reaction with l-tyrosine and l-DOPA
Conclusion:
It is presumed that arbutin could alleviate oxidative stress derived from the melanogenic pathway in the skin in addition to its function as a whitening agent in cosmetics. (Source: BMC Biochemistry)
Source: BMC Biochemistry - October 9, 2014 Category: Biochemistry Authors: Mika TadaMasahiro KohnoYoshimi Niwano Source Type: research
Comparative sequence analysis and mutagenesis of Ethylene Forming Enzyme (EFE) 2-oxoglutarate/Fe(II)-dependent dioxygenase homologs
Conclusion:
We provide further insight into the structure and function of the ethylene forming (EFE) - subclass of 2-oxoglutarate/Fe(II) dependent dioxygenases. We conclude that residues in addition to the 10 identified positions implicated in ethylene production by sequence comparison, are important for determining ethylene formation. We also demonstrate the use of an alternative EFE gene. The data from this study will provide the basis for directed protein engineering to enhance the ethylene production capability and properties of EFE. (Source: BMC Biochemistry)
Source: BMC Biochemistry - October 2, 2014 Category: Biochemistry Authors: Nina JohanssonKarl PerssonChrister LarssonJoakim Norbeck Source Type: research
Importance of extended protease substrate recognition motifs in steering BNIP-2 cleavage by human and mouse granzymes B
Conclusions:
Despite their high sequence similarity, and previously explained by their distinct tetrapeptide specificities observed, the substrate repertoires of mouse and human granzymes B only partially overlap. Here, we show that the substrate sequence context beyond the P4-P1 positions can influence orthologous granzyme B cleavage efficiencies to an unmatched extent. More specifically, in BNIP-2, the identical and hGrB optimal IEAD tetrapeptide substrate motif is targeted highly efficiently by mGrB, while this tetrapeptide motif is refractory towards mGrB cleavage in Bid. (Source: BMC Biochemistry)
Source: BMC Biochemistry - September 10, 2014 Category: Biochemistry Authors: Petra Van DammeKim PlasmanGiel VandemoorteleVeronique JonckheereSebastian Maurer-StrohKris Gevaert Source Type: research
Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS
Conclusion:
The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting. (Source: BMC Biochemistry)
Source: BMC Biochemistry - August 9, 2014 Category: Biochemistry Authors: Tao YiLan-Lan FanHong-Li ChenGuo-Yuan ZhuHau-Man SuenYi-Na TangLin ZhuChu ChuZhong-Zhen ZhaoHu-Biao Chen Source Type: research
The use of transformed IMR90 cell model to identify the potential extra-telomeric effects of hTERT in cell migration and DNA damage response
Conclusions:
Collectively, our study revealed the extra-telomeric effects of hTERT in cell migration and DDR during neoplastic transformation. (Source: BMC Biochemistry)
Source: BMC Biochemistry - August 7, 2014 Category: Biochemistry Authors: Xu CaoChiou KongKanchi MathiYoon LimValere Cacheux-RataboulXueying Wang Source Type: research
Chemical glycosylation of cytochrome c improves physical and chemical protein stability
Conclusion:
The results demonstrate that chemical glycosylation is a potentially valuable method to increase Cyt c stability during formulation and storage and potentially during its application after administration. (Source: BMC Biochemistry)
Source: BMC Biochemistry - August 6, 2014 Category: Biochemistry Authors: Yamixa DelgadoMoraima Morales-CruzJosé Hernández-RománYashira MartínezKai Griebenow Source Type: research
Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds
Conclusions:
Urease was purified from germinating Pisum Sativum L. seeds. The purification fold, yield, and molecular weight were determined. The effects of pH, concentration of enzyme, temperature, concentration of substrate, and storage period on urease activity were examined. This may provide an insight on the various aspects of the property of the enzyme. The significance of extracting urease from different sources could play a good role in understanding the metabolism of urea in plants. (Source: BMC Biochemistry)
Source: BMC Biochemistry - July 28, 2014 Category: Biochemistry Authors: Mohamed EL-HefnawyMohamed SakranAli IsmailEman Aboelfetoh Source Type: research
Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs
Conclusions:
Due to its secretion via multiple secretion pathways GLUC can find multiple applications as a research tool to study classical and non-conventional secretion. As GLUC can also be released from a reporter construct by internal signal peptide-mediated secretion it can be incorporated in a novel bicistronic secretion system. (Source: BMC Biochemistry)
Source: BMC Biochemistry - July 9, 2014 Category: Biochemistry Authors: Christin LuftJamie FreemanDavid ElliottNadia Al-TamimiJanos Kriston-ViziJacob HeintzeIda LindenschmidtBrian SeedRobin Ketteler Source Type: research
The basic amino acids in the coiled-coil domain of CIN85 regulate its interaction with c-Cbl and phosphatidic acid during epidermal growth factor receptor (EGFR) endocytosis
Point mutation of basic amino acids in the coiled-coil domain of the adaptor protein CIN85 indicate the importance of these residues for the interaction of CIN85 with c-Cbl and phosphatidic acid. (Source: BMC Biochemistry)
Source: BMC Biochemistry - July 8, 2014 Category: Biochemistry Authors: Xiudan ZhengJing ZhangKan Liao Source Type: research
Expression and characterization of a ß-fructofuranosidase from the parasitic protist Trichomonas vaginalis
Conclusions:
T. vaginalis has the coding capacity to produce an active beta-fructofuranosidase capable of hydrolyzing di- and trisaccharides containing a terminal, non-reducing fructose residue. Since we cloned this enzyme from cDNA, we know that the gene in question is transcribed. Furthermore, we could detect beta-fructofuranosidase activity in T. vaginalis cell lysates. Therefore, the inability of the organism to utilize sucrose as a carbon source cannot be explained by an inability to degrade sucrose. (Source: BMC Biochemistry)
Source: BMC Biochemistry - June 28, 2014 Category: Biochemistry Authors: Michael DirkxMichael BoyerPrajakta PradhanAndrew BrittinghamWayne Wilson Source Type: research
Expression and characterization of a beta-fructofuranosidase from the parasitic protist Trichomonas vaginalis
Conclusions:
T. vaginalis has the coding capacity to produce an active beta-fructofuranosidase capable of hydrolyzing di- and trisaccharides containing a terminal, non-reducing fructose residue. Since we cloned this enzyme from cDNA, we know that the gene in question is transcribed. Furthermore, we could detect beta-fructofuranosidase activity in T. vaginalis cell lysates. Therefore, the inability of the organism to utilize sucrose as a carbon source cannot be explained by an inability to degrade sucrose. (Source: BMC Biochemistry)
Source: BMC Biochemistry - June 28, 2014 Category: Biochemistry Authors: Michael DirkxMichael BoyerPrajakta PradhanAndrew BrittinghamWayne Wilson Source Type: research
