Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds

Conclusions:
Urease was purified from germinating Pisum Sativum L. seeds. The purification fold, yield, and molecular weight were determined. The effects of pH, concentration of enzyme, temperature, concentration of substrate, and storage period on urease activity were examined. This may provide an insight on the various aspects of the property of the enzyme. The significance of extracting urease from different sources could play a good role in understanding the metabolism of urea in plants.

Source: BMC Biochemistry - July 28, 2014 Category: Biochemistry Authors: Mohamed EL-HefnawyMohamed SakranAli IsmailEman Aboelfetoh Source Type: research