ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates

Conclusions: We discovered an ADP-ribosylargininyl activity of cholix toxin catalytic fragment from our studies in auto-ADP-ribosylation, which is mediated through diffusible intermediates. The lifetime of the hypothetical intermediate exceeds recorded and predicted lifetimes for the cognate oxocarbenium ion. Therefore, a diffusible strained form of NAD+ intermediate was proposed to react with arginine residues in a proximity dependent manner.

http://www.biomedcentral.com/1471-2091/15/26

Source: BMC Biochemistry - December 11, 2014 Category: Biochemistry Authors: Vicky SungChia-Lun Tsai Source Type: research

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